Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 169 80.7 2736954 99.0% 2709584 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,351,405 T→C T324A (ACC→GCC)  glpT ← sn‑glycerol 3‑phosphate:phosphate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,351,4050TC99.1% 387.4 / ‑5.4 108T324A (ACC→GCC) glpTsn‑glycerol 3‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (58/49);  total (59/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 108 total aligned reads displayed.

CCGATCAGCATCACAGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAACCTCTTTCA  >  NC_000913/2351269‑2351536
                                                                                                                                        |                                                                                                                                   
ccGATCAGCATCACAGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAATAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGc                                                                                                                                  >  1:103503/1‑140 (MQ=255)
   aTCAGCATCACAGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGcccc                                                                                                                               <  1:258662/140‑1 (MQ=255)
   aTCAGCATCACAGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGcccc                                                                                                                               >  2:371249/1‑140 (MQ=255)
      aGCATCACAGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACg                                                                                                                            <  1:361838/140‑1 (MQ=255)
        cATCACAGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATAACGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGt                                                                                                                          >  1:999797/1‑140 (MQ=255)
           cacaGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGc                                                                                                                       <  2:749495/140‑1 (MQ=255)
              aGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCAc                                                                                                                    >  2:153809/1‑140 (MQ=255)
                gACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACgg                                                                                                                  <  1:637362/140‑1 (MQ=255)
                 aCCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGa                                                                                                                 <  1:1177225/140‑1 (MQ=255)
                 aCCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGa                                                                                                                 <  1:1261622/140‑1 (MQ=255)
                  ccGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACgg                                                                                                                  >  2:638942/1‑138 (MQ=255)
                  ccGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACgg                                                                                                                  <  1:638942/138‑1 (MQ=255)
                  ccGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGaa                                                                                                                >  1:338977/1‑140 (MQ=255)
                     tAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGAc                                                                                                             >  1:650327/1‑140 (MQ=255)
                     tAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGAc                                                                                                             >  1:191589/1‑140 (MQ=255)
                     tAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGAc                                                                                                             >  1:535106/1‑140 (MQ=255)
                     tAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGAc                                                                                                             >  1:372962/1‑140 (MQ=255)
                     tAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCC‑GTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACt                                                                                                            >  1:265239/1‑140 (MQ=255)
                        aTCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGCCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACttt                                                                                                          >  1:551802/1‑140 (MQ=255)
                            ggAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATcc                                                                                                      >  1:797992/1‑140 (MQ=255)
                            ggAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATcc                                                                                                      >  1:1170718/1‑140 (MQ=255)
                            ggAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATcc                                                                                                      >  2:590220/1‑140 (MQ=255)
                             gAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCg                                                                                                     <  2:191589/140‑1 (MQ=255)
                             gAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCg                                                                                                     >  1:374331/1‑140 (MQ=255)
                                gCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACa                                                                                                  <  2:374331/140‑1 (MQ=255)
                                  cGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATc                                                                                                >  2:876745/1‑140 (MQ=255)
                                   gATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATcc                                                                                               <  1:533393/140‑1 (MQ=255)
                                    aTAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCa                                                                                              >  1:791354/1‑140 (MQ=255)
                                     tAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAg                                                                                             <  1:1062317/140‑1 (MQ=255)
                                      aaCAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGc                                                                                            <  2:535106/140‑1 (MQ=255)
                                              tACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAg                                                                                             >  2:1323125/1‑131 (MQ=255)
                                               aCAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCAcagca                                                                                   <  1:1160609/140‑1 (MQ=255)
                                                cAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATcc                                                                                               >  2:337632/1‑127 (MQ=255)
                                                cAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCAcagcag                                                                                  >  2:472764/1‑140 (MQ=255)
                                                 aaaTCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCaga                                                                                 <  2:1180195/140‑1 (MQ=255)
                                                   aTCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt                                                                               >  2:772799/1‑140 (MQ=255)
                                                   aTCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt                                                                               >  2:1211481/1‑140 (MQ=255)
                                                   aTCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt                                                                               <  2:1170718/140‑1 (MQ=255)
                                                   aTCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAATAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGt                                                                               <  2:103503/140‑1 (MQ=255)
                                                     cATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGc                                                                             >  1:10968/1‑140 (MQ=255)
                                                      atatCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGcc                                                                            <  2:791354/140‑1 (MQ=255)
                                                       tatCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGccc                                                                           >  1:289194/1‑140 (MQ=255)
                                                           gACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGaa                                                                       <  1:779743/140‑1 (MQ=255)
                                                            aCGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAAt                                                                      >  1:1335105/1‑140 (MQ=255)
                                                             cGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCTACATCCAGCCGCACAGCAGAGTGCCCGGAATa                                                                     >  1:343408/1‑140 (MQ=255)
                                                             cGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGa                                                                        <  2:1032386/137‑1 (MQ=255)
                                                             cGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGa                                                                        >  1:1032386/1‑137 (MQ=255)
                                                             cGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATa                                                                     <  2:551802/140‑1 (MQ=255)
                                                               gTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCAcagc                                                                                    <  1:314764/123‑1 (MQ=255)
                                                               gTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCAcagc                                                                                    >  2:314764/1‑123 (MQ=255)
                                                               gTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCgg                                                                         <  1:225025/134‑1 (MQ=255)
                                                               gTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCgg                                                                         >  2:225025/1‑134 (MQ=255)
                                                               gTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCg                                                                          <  2:811330/133‑1 (MQ=255)
                                                               gTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCg                                                                          >  1:811330/1‑133 (MQ=255)
                                                               gTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATAcc                                                                   >  2:775501/1‑140 (MQ=255)
                                                                 tGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTg                                                                 <  2:797992/140‑1 (MQ=255)
                                                                   ggTTTCCTGCCGGGTTCATCC‑GTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCat                                                              <  2:265239/140‑1 (MQ=255)
                                                                   ggTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCa                                                               <  1:876745/140‑1 (MQ=255)
                                                                       aCCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATAtt                                                           <  2:755157/140‑1 (MQ=255)
                                                                        ccTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTc                                                          >  2:522898/1‑140 (MQ=255)
                                                                           gCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATa                                                       <  1:213115/140‑1 (MQ=255)
                                                                                 ttCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGaa                                                 >  1:528952/1‑140 (MQ=255)
                                                                                    aTCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTa                                              >  1:358897/1‑140 (MQ=255)
                                                                                     tCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAg                                             >  2:659924/1‑140 (MQ=255)
                                                                                      ccAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAgg                                            >  2:1029211/1‑140 (MQ=255)
                                                                                          tAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCa                                        >  2:256918/1‑140 (MQ=255)
                                                                                            aaCGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCagg                                      <  1:1211481/140‑1 (MQ=255)
                                                                                                    tCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGGGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTa                              >  1:741239/1‑140 (MQ=255)
                                                                                                    tCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTa                              >  1:55622/1‑140 (MQ=255)
                                                                                                           ggTCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAgcg                       >  2:1314270/1‑140 (MQ=255)
                                                                                                             tCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGcgcg                     >  1:177464/1‑140 (MQ=255)
                                                                                                             tCACCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGcgcg                     <  1:1315953/140‑1 (MQ=255)
                                                                                                               aCCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGaa                   <  1:775501/140‑1 (MQ=255)
                                                                                                               aCCAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGaa                   >  2:211249/1‑140 (MQ=255)
                                                                                                                ccAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGaaa                  <  1:256918/140‑1 (MQ=255)
                                                                                                                ccAGTGTCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGaaa                  <  1:522898/140‑1 (MQ=255)
                                                                                                                    tgtCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCt              <  2:358897/140‑1 (MQ=255)
                                                                                                                    tgtCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCt              >  1:1228053/1‑140 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACNGTTGCCACGGAANACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTa            >  2:831298/1‑140 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGa                    >  2:1357178/1‑132 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGa                    <  1:1357178/132‑1 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTa            >  2:709842/1‑140 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTa            >  2:680223/1‑140 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTa            >  2:1325641/1‑140 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTa            >  2:1054617/1‑140 (MQ=255)
                                                                                                                      tCATAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTa            >  2:122863/1‑140 (MQ=255)
                                                                                                                        aTAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAAc          <  2:55622/140‑1 (MQ=255)
                                                                                                                         tAAAGAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAAcc         <  1:153809/140‑1 (MQ=255)
                                                                                                                             gAAAACGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAACCTCtt     <  1:709842/140‑1 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCt              <  2:1134363/130‑1 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCt              >  1:1134363/1‑130 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAAcc         <  2:1057674/135‑1 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAAcc         >  1:1057674/1‑135 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAAc          >  1:506500/1‑134 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAAc          <  2:506500/134‑1 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAACCTCttt    >  2:55177/1‑140 (MQ=255)
                                                                                                                              aaaaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAACCTCttt    >  2:216300/1‑140 (MQ=255)
                                                                                                                                aaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTaa           <  2:645569/131‑1 (MQ=255)
                                                                                                                                aaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTaa           >  1:645569/1‑131 (MQ=255)
                                                                                                                                aaCGCCGGCTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAACCTCTTTCa  >  1:772937/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                   
CCGATCAGCATCACAGGACCGTAGATCAGGAAGCCGATAACAATCATACAAATCATATCGACGGTTGGGTTACCTGCCGGGTTCATCCAGTAAACGATAGTCGCGATGGTCACCAGTGTCATAAAGAAAACGCCGGTTGCCCCACGGTTGCCACGGAAGACTTTATCCGACATCCAGCCGCACAGCAGAGTGCCCGGAATACCTGCATATTCATAAAGGAAGTAGGCCCAGGAGGATTTATCTAGCGCGAAATGCTTAACCTCTTTCA  >  NC_000913/2351269‑2351536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A