Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	169	80.7	2736954	99.0%	2709584	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,443,211	T→C	V483A (GTC→GCC)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,443,211	0	T	C	98.1%	355.5 / ‑3.0	103	V483A (GTC→GCC)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (49/52); total (50/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 105 total aligned reads displayed.

AAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGAT  >  NC_000913/2443075‑2443344

aaGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAg                                                                                                                                    >  2:561026/1‑140 (MQ=255)

aaGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAg                                                                                                                                    >  2:820924/1‑140 (MQ=255)

aaGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAg                                                                                                                                    >  2:759577/1‑140 (MQ=255)

 aGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGATTCTCCCTGTGTATTCGGTTGCCGGGCAGGCCAGc                                                                                                                                   <  2:308025/140‑1 (MQ=255)

  ggATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGcc                                                                                                                                  <  2:842625/140‑1 (MQ=255)

    aTGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCat                                                                                                                                >  1:747325/1‑140 (MQ=255)

    aTGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCat                                                                                                                                >  1:1198647/1‑140 (MQ=255)

       aCTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATAtt                                                                                                                             >  2:1830/1‑140 (MQ=255)

       aCTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATAtt                                                                                                                             >  1:663968/1‑140 (MQ=255)

       aCTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATAtt                                                                                                                             >  2:620976/1‑140 (MQ=255)

       aCTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATAtt                                                                                                                             >  2:1128903/1‑140 (MQ=255)

       aCTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATAtt                                                                                                                             <  1:527580/140‑1 (MQ=255)

        cTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTc                                                                                                                            >  2:1242077/1‑140 (MQ=255)

           ttggttgTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGa                                                                                                                         <  1:561026/140‑1 (MQ=255)

           ttggttgTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGa                                                                                                                         >  1:619263/1‑140 (MQ=255)

             ggttgTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACa                                                                                                                       >  2:756166/1‑140 (MQ=255)

                tgttgAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACg                                                                                                                    <  1:1274638/140‑1 (MQ=255)

                  ttgAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGcc                                                                                                                  >  2:298731/1‑140 (MQ=255)

                      aTTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGaa                                                                                                              <  1:756166/140‑1 (MQ=255)

                       ttttGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAAt                                                                                                             >  1:111897/1‑140 (MQ=255)

                           gCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGc                                                                                                         <  1:620976/140‑1 (MQ=255)

                           gCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGc                                                                                                         <  1:1830/140‑1 (MQ=255)

                               gagaTCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCagag                                                                                                     <  1:298807/140‑1 (MQ=255)

                               gagaTCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCagag                                                                                                     <  2:619263/140‑1 (MQ=255)

                                agaTCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAACCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGc                                                                                                    >  2:76930/1‑140 (MQ=255)

                                 gaTCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCt                                                                                                   <  1:811465/140‑1 (MQ=255)

                                           caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtg                                                                                         <  2:143852/140‑1 (MQ=255)

                                           caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtg                                                                                         <  1:629865/140‑1 (MQ=255)

                                            aaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtgc                                                                                        >  1:1286729/1‑140 (MQ=255)

                                              cacacaGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtgctg                                                                                      >  1:566316/1‑140 (MQ=255)

                                                cacaGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtgctgtg                                                                                    <  1:341302/140‑1 (MQ=255)

                                                 acaGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtgctgtgc                                                                                   >  1:282989/1‑140 (MQ=255)

                                                 acaGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtgctgtgc                                                                                   >  2:1345954/1‑140 (MQ=255)

                                                   aGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTa                                                                                 <  2:747325/140‑1 (MQ=255)

                                                    gCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTAt                                                                                >  1:881264/1‑140 (MQ=255)

                                                     cGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATg                                                                               >  2:14268/1‑140 (MQ=255)

                                                     cGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATg                                                                               >  1:98798/1‑140 (MQ=255)

                                                     cGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATg                                                                               >  2:1098792/1‑140 (MQ=255)

                                                      gTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGa                                                                              >  2:565219/1‑140 (MQ=255)

                                                        aGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCGGGTGCTGTGCTATGACg                                                                            >  2:755204/1‑140 (MQ=255)

                                                        aGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtg                                                                                         >  2:414398/1‑127 (MQ=255)

                                                        aGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGgtg                                                                                         <  1:414398/127‑1 (MQ=255)

                                                        aGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACg                                                                            >  2:284683/1‑140 (MQ=255)

                                                        aGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTAGCAGAGCTGAAGCAGGTGCTGTGCTATGACg                                                                            >  1:1282142/1‑140 (MQ=255)

                                                        aGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGCGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACg                                                                            >  1:949597/1‑140 (MQ=255)

                                                               cTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTAtct                                                                     >  1:667129/1‑140 (MQ=255)

                                                               cTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTAtct                                                                     >  2:880436/1‑140 (MQ=255)

                                                                  gCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCAc                                                                  >  1:257132/1‑140 (MQ=255)

                                                                   cGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACg                                                                 >  1:625353/1‑140 (MQ=255)

                                                                   cGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACg                                                                 <  2:1063238/140‑1 (MQ=255)

                                                                      aCGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCca                                                              >  1:779921/1‑140 (MQ=255)

                                                                      aCGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCca                                                              >  2:6685/1‑140 (MQ=255)

                                                                       cGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCcac                                                             >  2:1178525/1‑140 (MQ=255)

                                                                           cATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACaaaa                                                         <  1:1242077/140‑1 (MQ=255)

                                                                            aTCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAAt                                                        >  1:984575/1‑140 (MQ=255)

                                                                            aTCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAAt                                                        >  2:967026/1‑140 (MQ=255)

                                                                               aaaTCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCg                                                     <  1:6685/140‑1 (MQ=255)

                                                                               aaaTCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCg                                                     <  2:111897/140‑1 (MQ=255)

                                                                                aaTCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCgg                                                    <  1:820924/140‑1 (MQ=255)

                                                                                 aTCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGc                                                   <  2:984575/140‑1 (MQ=255)

                                                                                 aTCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGc                                                   <  1:1128903/140‑1 (MQ=255)

                                                                                  tCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCg                                                  >  1:363693/1‑140 (MQ=255)

                                                                                     gCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGa                                                 <  2:768570/138‑1 (MQ=255)

                                                                                     gCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGa                                                 >  1:768570/1‑138 (MQ=255)

                                                                                     gCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAAt                                               <  1:1259979/140‑1 (MQ=255)

                                                                                     gCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAAt                                               <  1:1178525/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  2:779921/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  2:1286729/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  1:1022223/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  1:1345954/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  2:667129/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  1:759577/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  2:363693/140‑1 (MQ=255)

                                                                                        gATTCAGCCAAACGTCGACTCTCCAGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  1:516794/140‑1 (MQ=255)

                                                                                         aTTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAc                                           <  1:14268/140‑1 (MQ=255)

                                                                                         aTTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAc                                           <  1:1098792/140‑1 (MQ=255)

                                                                                          ttCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAca                                          <  2:1230341/140‑1 (MQ=255)

                                                                                          ttCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGATATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAca                                          <  2:663968/140‑1 (MQ=255)

                                                                                           tCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            >  2:436274/1‑137 (MQ=255)

                                                                                           tCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                            <  1:436274/137‑1 (MQ=255)

                                                                                           tCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAcat                                         >  1:296042/1‑140 (MQ=255)

                                                                                             aGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAcatca                                       <  2:881264/140‑1 (MQ=255)

                                                                                             aGCCAAACGTCGACTCTCCCGGCGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAcatca                                       <  2:949597/140‑1 (MQ=255)

                                                                                               ccAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCAtt                                     <  2:625353/140‑1 (MQ=255)

                                                                                                   aCGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTAt                                 <  1:836575/140‑1 (MQ=255)

                                                                                                    cGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTAtt                                <  2:282989/140‑1 (MQ=255)

                                                                                                    cGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTAtt                                >  2:1085474/1‑140 (MQ=255)

                                                                                                      tCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTgg                              <  1:1085474/140‑1 (MQ=255)

                                                                                                       cGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGt                             <  2:1282142/140‑1 (MQ=255)

                                                                                                         aCTCTCCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGc                           >  2:1032134/1‑140 (MQ=255)

                                                                                                           tctcCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGt                                   >  2:1154906/1‑130 (MQ=255)

                                                                                                           tctcCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGt                                   <  1:1154906/130‑1 (MQ=255)

                                                                                                             tcCCGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGt                       >  1:1273666/1‑140 (MQ=255)

                                                                                                               ccGGTGTATTCGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTa                     >  2:410821/1‑140 (MQ=255)

                                                                                                                       ttCGGTGGCCGGGCAGGCCAGCCATATTCCGCCAACGCCGGAATTGGCGGAGCTGAAGCAGGTGCTGGGCTAGGTGGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATcgcg             <  2:199236/140‑1 (MQ=255)

                                                                                                                         cGGTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGc           <  2:257132/140‑1 (MQ=255)

                                                                                                                           gTTGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAg         <  1:1032134/140‑1 (MQ=255)

                                                                                                                            ttGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGc        >  1:162018/1‑140 (MQ=255)

                                                                                                                             tGCCGGGCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCg       >  1:807512/1‑140 (MQ=255)

                                                                                                                                  ggCAGGCCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGAt  <  1:124142/140‑1 (MQ=255)

AAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGAT  >  NC_000913/2443075‑2443344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A