Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 169 80.7 2736954 99.0% 2709584 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,602,017 (C)7→6 coding (200/2019 nt) hyfB → hydrogenase 4 component B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,602,0110C.98.8% 345.2 / ‑2.4 82coding (194/2019 nt)hyfBhydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (39/42);  total (40/42)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601873‑2602143
                                                                                                                                          |                                                                                                                                    
tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGcc                                                                                                                                     <  1:493444/140‑1 (MQ=255)
  gCTTCGCTGTTTTTACTCGGTCTGGTCAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGccc                                                                                                                                    <  1:104053/139‑1 (MQ=255)
  gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGccc                                                                                                                                    >  2:104053/1‑139 (MQ=255)
    ttCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGcccccc                                                                                                                                 >  1:454700/1‑140 (MQ=255)
     tCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGcccccct                                                                                                                                >  2:305459/1‑139 (MQ=255)
       gCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCttt                                                                                                                              >  2:1139949/1‑140 (MQ=255)
            ttttACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAAt                                                                                                                        >  1:348602/1‑140 (MQ=255)
              ttACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAAttt                                                                                                                      <  1:790725/140‑1 (MQ=255)
                aCTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGc                                                                                                                    <  1:305459/140‑1 (MQ=255)
                aCTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGc                                                                                                                    <  2:348602/140‑1 (MQ=255)
                     gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGAtt                                                                                                               >  2:214893/1‑140 (MQ=255)
                       cTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTa                                                                                                             >  1:225626/1‑140 (MQ=255)
                          gACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAAcc                                                                                                          >  1:1135691/1‑140 (MQ=255)
                              gACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                                                                                      >  1:1012839/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTa                                                                                                             <  2:16836/132‑1 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTa                                                                                                             >  1:16836/1‑132 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  1:552337/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  2:867694/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  2:1200765/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  2:769829/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  1:148045/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  2:409945/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  1:1255736/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     >  2:46011/1‑140 (MQ=255)
                               aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     <  2:737823/140‑1 (MQ=255)
                                 tGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   <  2:225626/140‑1 (MQ=255)
                                 tGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   <  1:526834/140‑1 (MQ=255)
                                    cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                       <  2:1084115/133‑1 (MQ=255)
                                    cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  1:1084115/1‑133 (MQ=255)
                                      aTTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGAt                                                                                              <  1:1057948/140‑1 (MQ=255)
                                      aTTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGAt                                                                                              <  2:1135691/140‑1 (MQ=255)
                                         aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGc                                                                                           >  2:716397/1‑140 (MQ=255)
                                         aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGc                                                                                           >  2:261041/1‑140 (MQ=255)
                                             tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   >  1:511105/1‑128 (MQ=255)
                                             tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   <  2:511105/128‑1 (MQ=255)
                                             tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                       >  1:1345169/1‑140 (MQ=255)
                                             tttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                       >  1:915177/1‑140 (MQ=255)
                                              ttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      <  2:818066/140‑1 (MQ=255)
                                              ttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      <  1:93682/140‑1 (MQ=255)
                                              ttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      <  1:1215345/140‑1 (MQ=255)
                                                    cATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCTGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTATCCCTGCGAATGGAAAGCCTCTCGGCAtt                                                                                <  2:813954/140‑1 (MQ=255)
                                                     aTCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAttt                                                                               <  2:207171/140‑1 (MQ=255)
                                                         caTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATgg                                                                           >  1:1134478/1‑140 (MQ=255)
                                                           tCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                         <  2:915177/140‑1 (MQ=255)
                                                                     gcggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtct                                                               <  1:261041/140‑1 (MQ=255)
                                                                      cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtctc                                                              >  1:1355899/1‑140 (MQ=255)
                                                                      cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtctc                                                              <  1:46011/140‑1 (MQ=255)
                                                                            gATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCt                                                        >  1:814143/1‑140 (MQ=255)
                                                                                ggCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                                    <  1:867694/140‑1 (MQ=255)
                                                                                     catccGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               <  2:1255736/140‑1 (MQ=255)
                                                                                     catcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               <  2:220959/140‑1 (MQ=255)
                                                                                     catcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               <  2:1355899/140‑1 (MQ=255)
                                                                                      atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCTTTGCTGGTGGTGGtt                                              >  2:859680/1‑140 (MQ=255)
                                                                                       tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               >  2:712597/1‑138 (MQ=255)
                                                                                       tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               <  1:712597/138‑1 (MQ=255)
                                                                                       tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGttt                                             >  1:1057981/1‑140 (MQ=255)
                                                                                         aGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  1:716397/140‑1 (MQ=255)
                                                                                         aGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  2:454700/140‑1 (MQ=255)
                                                                                         aGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  1:214893/140‑1 (MQ=255)
                                                                                         aGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  2:148045/140‑1 (MQ=255)
                                                                                           cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  2:193946/140‑1 (MQ=255)
                                                                                           cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         >  2:1313106/1‑140 (MQ=255)
                                                                                           cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         >  1:193946/1‑140 (MQ=255)
                                                                                              aaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctct                                      <  2:1345169/140‑1 (MQ=255)
                                                                                               aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  1:1139949/140‑1 (MQ=255)
                                                                                               aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  1:1200765/140‑1 (MQ=255)
                                                                                               aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  1:769829/140‑1 (MQ=255)
                                                                                                ttACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTctctct                                    >  1:900366/1‑140 (MQ=255)
                                                                                                   cGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           >  1:629842/1‑130 (MQ=255)
                                                                                                   cGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  2:629842/130‑1 (MQ=255)
                                                                                                     cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                               <  1:643297/140‑1 (MQ=255)
                                                                                                     cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                               <  2:552337/140‑1 (MQ=255)
                                                                                                      aaTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              <  1:1313106/140‑1 (MQ=255)
                                                                                                        ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtct                                        <  2:473365/128‑1 (MQ=255)
                                                                                                        ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtct                                        >  1:473365/1‑128 (MQ=255)
                                                                                                        ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTg                            >  1:123450/1‑140 (MQ=255)
                                                                                                                 ggTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATg                   <  2:814143/140‑1 (MQ=255)
                                                                                                                  gtgtAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATgc                  >  2:1118832/1‑140 (MQ=255)
                                                                                                                  gtgtAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATgc                  >  1:773247/1‑140 (MQ=255)
                                                                                                                     tAACTTTAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGcgcg               <  2:1134478/140‑1 (MQ=255)
                                                                                                                          ttAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          <  2:1057981/140‑1 (MQ=255)
                                                                                                                           tAGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACg         >  2:619719/1‑140 (MQ=255)
                                                                                                                            aGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGa        >  1:1063323/1‑140 (MQ=255)
                                                                                                                            aGTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGCGGCGGCTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGa        >  1:930739/1‑140 (MQ=255)
                                                                                                                             gTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAg       >  2:248329/1‑140 (MQ=255)
                                                                                                                             gTCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAg       >  2:1063754/1‑140 (MQ=255)
                                                                                                                              tCGCCCGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAgg      >  2:181033/1‑140 (MQ=255)
                                                                                                                                  ccGTTTTG‑CCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCaa  <  1:248329/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
TGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601873‑2602143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A