Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	169	80.7	2736954	99.0%	2709584	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,283	G→A	Q194* (CAG→TAG)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,283	0	G	A	98.4%	197.9 / ‑3.7	64	Q194* (CAG→TAG)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (31/31); minor base C (1/0); total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

GTGACACCCGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGC  >  NC_000913/2973162‑2973416

gTGACACCCGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGcgccgccgc                                                                                                                     <  1:3331/140‑1 (MQ=255)

gTGACACCCGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGcgccgccgc                                                                                                                     >  2:579060/1‑140 (MQ=255)

        cGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTgg                                                                                                             <  1:701719/140‑1 (MQ=255)

        cGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTgg                                                                                                             <  2:670139/140‑1 (MQ=255)

        cGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTgg                                                                                                             <  2:221992/140‑1 (MQ=255)

        cGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTgg                                                                                                             <  1:127756/140‑1 (MQ=255)

         gCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTggg                                                                                                            <  1:579060/140‑1 (MQ=255)

         gCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTCCGCCATAGCCAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTggg                                                                                                            <  2:1220968/140‑1 (MQ=255)

            ccccAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAAt                                                                                                         <  2:333378/140‑1 (MQ=255)

               cAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGttt                                                                                                               <  2:970681/131‑1 (MQ=255)

               cAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGttt                                                                                                               >  1:970681/1‑131 (MQ=255)

                aGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAg                                                                                                     <  2:328755/140‑1 (MQ=255)

                 gAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCATGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTg                                                                                                              <  2:1219683/130‑1 (MQ=255)

                 gAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTg                                                                                                              >  1:1219683/1‑130 (MQ=255)

                  aaTAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGat                                                                                                   >  1:1208401/1‑140 (MQ=255)

                    tAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGttt                                                                                                               <  2:656717/126‑1 (MQ=255)

                    tAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGGGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGttt                                                                                                               >  1:656717/1‑126 (MQ=255)

                         tGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCa                                                                                            >  2:310099/1‑140 (MQ=255)

                           gTGCCCACCAGCGAAAAACGCGTTTCACCATTGCACCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAAc                                                                                          <  2:1101052/140‑1 (MQ=255)

                            tGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACg                                                                                         >  2:1208739/1‑140 (MQ=255)

                            tGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACg                                                                                         >  1:252534/1‑140 (MQ=255)

                            tGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACg                                                                                         >  2:1342216/1‑140 (MQ=255)

                              cccACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGTGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTTCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGAc                                                                                       <  2:1143492/140‑1 (MQ=255)

                                caccaGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCg                                                                                     >  1:1141517/1‑140 (MQ=255)

                                   caGCGTAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  2:1208401/140‑1 (MQ=255)

                                   caGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  1:1342216/140‑1 (MQ=255)

                                   caGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  2:135963/140‑1 (MQ=255)

                                   caGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  1:1208739/140‑1 (MQ=255)

                                   caGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  1:32607/140‑1 (MQ=255)

                                   caGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  1:310099/140‑1 (MQ=255)

                                   caGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  1:1322497/140‑1 (MQ=255)

                                   caGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcac                                                                                  <  1:1363184/140‑1 (MQ=255)

                                     gCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGCCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGcacca                                                                                <  1:565667/140‑1 (MQ=255)

                                             cgcgTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGc                                                                        <  2:588193/140‑1 (MQ=255)

                                                 tttCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGt                                                                    >  2:76467/1‑140 (MQ=255)

                                                       cATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCAt                                                              <  2:1141517/140‑1 (MQ=255)

                                                            cgcCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTCACCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGc                                                         >  2:1218821/1‑140 (MQ=255)

                                                            cgcCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGc                                                         >  1:1287788/1‑140 (MQ=255)

                                                              ccATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCa                                                       >  1:1034134/1‑140 (MQ=255)

                                                                       ggTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGcgacga                                              <  1:375678/140‑1 (MQ=255)

                                                                       ggTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGcgacga                                              >  1:1147853/1‑140 (MQ=255)

                                                                        gTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAg                                             >  1:1164945/1‑140 (MQ=255)

                                                                         tGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAgg                                            >  2:1327631/1‑140 (MQ=255)

                                                                           cAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGAc                                          >  2:339635/1‑140 (MQ=255)

                                                                           cAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGAc                                          >  2:777895/1‑140 (MQ=255)

                                                                                 ttCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCa                                    >  1:461376/1‑140 (MQ=255)

                                                                                  tCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAg                                   <  2:252534/140‑1 (MQ=255)

                                                                                   cAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGt                                  >  1:776790/1‑140 (MQ=255)

                                                                                   cAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGt                                  >  1:711846/1‑140 (MQ=255)

                                                                                     ggAAACTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTca                                >  2:1031233/1‑140 (MQ=255)

                                                                                       aaaCTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagc                              <  1:1327631/140‑1 (MQ=255)

                                                                                         aCTGCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagcca                            >  1:431708/1‑140 (MQ=255)

                                                                                            gCGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGca                         >  2:159194/1‑140 (MQ=255)

                                                                                             cGGGTCATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGcac                        >  2:235310/1‑140 (MQ=255)

                                                                                                  cATGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCAcaccac                   >  1:332842/1‑140 (MQ=255)

                                                                                                   aTGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc                  <  2:1287788/140‑1 (MQ=255)

                                                                                                   aTGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc                  <  1:159194/140‑1 (MQ=255)

                                                                                                    tGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag                           >  2:1030726/1‑130 (MQ=255)

                                                                                                    tGTTGATGAGATTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag                           <  1:1030726/130‑1 (MQ=255)

                                                                                                             gaTTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGaa        <  1:235310/140‑1 (MQ=255)

                                                                                                              aTTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc                  <  2:387679/129‑1 (MQ=255)

                                                                                                              aTTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc                  >  1:387679/1‑129 (MQ=255)

                                                                                                              aTTCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAAc       >  2:1261067/1‑140 (MQ=255)

                                                                                                               ttCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAAcc      >  2:193441/1‑140 (MQ=255)

                                                                                                                tCCAGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCg     >  2:938267/1‑140 (MQ=255)

                                                                                                                   aGGACTACCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGc  >  2:780647/1‑140 (MQ=255)

GTGACACCCGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGC  >  NC_000913/2973162‑2973416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A