Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 169 80.7 2736954 99.0% 2709584 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,179,863 T→C S40S (AGT→AGC)  ygiB → DUF1190 domain‑containing protein YgiB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,179,8630TC98.8% 308.3 / NA 85S40S (AGT→AGC) ygiBDUF1190 domain‑containing protein YgiB
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (37/47);  total (37/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGT  >  NC_000913/3179726‑3179998
                                                                                                                                         |                                                                                                                                       
tGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGcga                                                                                                                                       >  2:928974/1‑140 (MQ=255)
tGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGcga                                                                                                                                       >  2:494339/1‑140 (MQ=255)
tGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGcga                                                                                                                                       >  1:73650/1‑140 (MQ=255)
 ggTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGcgat                                                                                                                                      <  1:336523/140‑1 (MQ=255)
  gTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGcgatg                                                                                                                                     >  1:623994/1‑140 (MQ=255)
  gTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGcgatg                                                                                                                                     <  1:253294/140‑1 (MQ=255)
  gTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACCGTTTTTATGCTGGCTGGCTGTGAAAAGAGcgatg                                                                                                                                     >  2:520311/1‑140 (MQ=255)
    cccGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACTCCATCTGACACCAGTCGCTCTTGCGGTTGCCACTG‑TTTTATGCTGGCTGGCTGTGAAAAGAGCGATGaaa                                                                                                                                  >  2:1012201/1‑140 (MQ=255)
     ccGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGcgatggaa                                                                                                                                  <  1:766987/140‑4 (MQ=255)
     ccGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGaaa                                                                                                                                  <  2:623994/140‑1 (MQ=255)
       ggAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACa                                                                                                                                <  1:972482/140‑1 (MQ=255)
           gACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCGCGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAgtgt                                                                                                                            <  1:304260/140‑1 (MQ=255)
           gACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTCCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAgtgt                                                                                                                            <  2:1203437/140‑1 (MQ=255)
            aCAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGtc                                                                                                                           >  1:581722/1‑140 (MQ=255)
             cAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGtct                                                                                                                          <  1:494339/140‑1 (MQ=255)
             cAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGtct                                                                                                                          <  2:792884/140‑1 (MQ=255)
             cAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGtct                                                                                                                          <  2:88440/140‑1 (MQ=255)
                aaaTGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGtctctc                                                                                                                       <  1:64093/140‑1 (MQ=255)
                          gACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAgt                                                                                                                              <  2:1233420/123‑1 (MQ=255)
                          gACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAgt                                                                                                                              >  1:1233420/1‑123 (MQ=255)
                                     tACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGt                                                                                                  >  1:76719/1‑140 (MQ=255)
                                      aCGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGtt                                                                                                 <  1:745637/140‑1 (MQ=255)
                                       cGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTc                                                                                                <  2:76719/140‑1 (MQ=255)
                                          cACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGc                                                                                             >  2:337617/1‑140 (MQ=255)
                                           aCGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCt                                                                                            >  1:975110/1‑140 (MQ=255)
                                            cGCATCGTTCCGCAAAAACTGGAGCGCACTCCATCTGACACCAGTCGCTCTTGCGGTTGCCACTG‑TTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGc                                                                                          <  1:1012201/140‑1 (MQ=255)
                                            cGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTg                                                                                           >  1:402476/1‑140 (MQ=255)
                                                   ttCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAAccc                                                                                    >  1:397903/1‑140 (MQ=255)
                                                    tCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGtt                                                                                                 <  2:102630/126‑1 (MQ=255)
                                                    tCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGtt                                                                                                 >  1:102630/1‑126 (MQ=255)
                                                    tCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCa                                                                                   >  2:38005/1‑140 (MQ=255)
                                                    tCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCa                                                                                   >  2:1306103/1‑140 (MQ=255)
                                                        cAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCa                                                                               >  2:632947/1‑140 (MQ=255)
                                                         aaaaaCTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCaa                                                                              <  1:94238/140‑1 (MQ=255)
                                                          aaaaCTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCaaa                                                                             <  2:1265767/140‑1 (MQ=255)
                                                             aCTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAgc                                                                          >  1:376731/1‑140 (MQ=255)
                                                                ggAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGcc                                                                       <  1:38005/140‑1 (MQ=255)
                                                                ggAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGcc                                                                       <  1:632947/140‑1 (MQ=255)
                                                                ggAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGcc                                                                       <  2:1141286/140‑1 (MQ=255)
                                                                ggAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGcc                                                                       <  2:397903/140‑1 (MQ=255)
                                                                   gcgcACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGaa                                                                    >  2:34410/1‑140 (MQ=255)
                                                                      cACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAgcg                                                                         >  2:678137/1‑132 (MQ=255)
                                                                      cACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAgcg                                                                         <  1:678137/132‑1 (MQ=255)
                                                                       aCGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTa                                                                <  2:402476/140‑1 (MQ=255)
                                                                       aCGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTa                                                                <  2:376731/140‑1 (MQ=255)
                                                                        cGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTac                                                               <  1:983610/140‑1 (MQ=255)
                                                                         gCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAgcg                                                                         >  2:221541/1‑129 (MQ=255)
                                                                         gCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAgcg                                                                         <  1:221541/129‑1 (MQ=255)
                                                                          ccATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTacca                                                             <  2:896544/140‑1 (MQ=255)
                                                                          ccATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTacca                                                             <  1:738446/140‑1 (MQ=255)
                                                                            aTCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTaccacc                                                           <  2:506867/140‑1 (MQ=255)
                                                                            aTCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTaccacc                                                           <  2:1100541/140‑1 (MQ=255)
                                                                                 acacCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGGCTGTTCAGCTGCAAACCCAGGCAAAAGCGcc                                                                       <  1:950112/123‑1 (MQ=255)
                                                                                 acacCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGcc                                                                       >  2:950112/1‑123 (MQ=255)
                                                                                 acacCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTacca                                                             <  1:78299/133‑1 (MQ=255)
                                                                                 acacCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTacca                                                             >  2:78299/1‑133 (MQ=255)
                                                                                   acCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTacca                                                             >  1:364567/1‑131 (MQ=255)
                                                                                   acCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTacca                                                             <  2:364567/131‑1 (MQ=255)
                                                                                     cAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTacaac                                                  >  1:1232833/1‑140 (MQ=255)
                                                                                     cAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTacaac                                                  >  2:699747/1‑140 (MQ=255)
                                                                                     cAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTacaac                                                  >  2:481978/1‑140 (MQ=255)
                                                                                      aGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTacaaca                                                 <  2:73650/140‑1 (MQ=255)
                                                                                      aGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTacaaca                                                 <  1:720029/140‑1 (MQ=255)
                                                                                       gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAATAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTAcaacaa                                                >  1:169554/1‑140 (MQ=255)
                                                                                       gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTaccacc                                                           <  1:773386/129‑1 (MQ=255)
                                                                                       gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTaccacc                                                           >  2:773386/1‑129 (MQ=255)
                                                                                       gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTAcaacaa                                                >  2:772189/1‑140 (MQ=255)
                                                                                       gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTAcaacaa                                                >  1:560234/1‑140 (MQ=255)
                                                                                       gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTAcaacaa                                                >  2:1163741/1‑140 (MQ=255)
                                                                                       gTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGGACCACCGCGTAcaacaa                                                >  2:1215243/1‑140 (MQ=255)
                                                                                        tCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAAt                                               <  1:284752/140‑1 (MQ=255)
                                                                                        tCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAAt                                               <  2:186538/140‑1 (MQ=255)
                                                                                                gcgGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCTCCGAATGTACCACCGCGTACAACAATGCGCTGaa                                       >  2:90195/1‑140 (MQ=255)
                                                                                                  ggTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAaag                                     >  2:1339024/1‑140 (MQ=255)
                                                                                                  ggTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAaag                                     <  1:1163741/140‑1 (MQ=255)
                                                                                                    ttGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAaagaa                                   <  1:34410/140‑1 (MQ=255)
                                                                                                      gCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGc                                 >  1:1290400/1‑140 (MQ=255)
                                                                                                         aCTGTTTTTATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGa                              <  1:1339024/140‑1 (MQ=255)
                                                                                                             tttttATGCTGGCTGGCTGTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGt                          >  2:1354445/1‑140 (MQ=255)
                                                                                                                     ctggctggctgTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTa                         >  1:1289869/1‑133 (MQ=255)
                                                                                                                     ctggctggctgTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTa                         <  2:1289869/133‑1 (MQ=255)
                                                                                                                       ggctggctgTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGa                >  2:1211443/1‑140 (MQ=255)
                                                                                                                       ggctggctgTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGa                >  1:1107886/1‑140 (MQ=255)
                                                                                                                       ggctggctgTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGa                >  2:244791/1‑140 (MQ=255)
                                                                                                                       ggctggctgTGAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGa                >  2:811128/1‑140 (MQ=255)
                                                                                                                             ctgTGAAAATAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACg          <  2:169554/140‑1 (MQ=255)
                                                                                                                              tgtgAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGc         <  2:607523/140‑1 (MQ=255)
                                                                                                                              tgtgAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGc         <  1:1215243/140‑1 (MQ=255)
                                                                                                                                tgAAAAGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCa       <  1:520311/140‑1 (MQ=255)
                                                                                                                                  aaaaGAGCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCAcc     <  2:560234/140‑1 (MQ=255)
                                                                                                                                     agagCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGt  <  2:581722/140‑1 (MQ=255)
                                                                                                                                     agagCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGt  <  1:811128/140‑1 (MQ=255)
                                                                                                                                      gagCGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCa       <  2:28896/134‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TGGTCCCGGAAGACAAAAATGAAACGGACAAAATCCATACGCCACGCATCGTTCCGCAAAAACTGGAGCGCACGCCATCTGACACCAGTCGCTCTCGCGGTTGCCACTGTTTTTATGCTGGCTGGCTGTGAAAAGAGTGATGAAACAGTGTCTCTCTATCAAAATGCTGACGACTGTTCAGCTGCAAACCCAGGCAAAAGCGCCGAATGTACCACCGCGTACAACAATGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGT  >  NC_000913/3179726‑3179998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A