Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,720,255	T→C	V127A (GTA→GCA)	slyB →	outer membrane lipoprotein SlyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,255	0	T	C	100.0%	101.8 / NA	29	V127A (GTA→GCA)	slyB	outer membrane lipoprotein SlyB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/17); total (12/17)

ACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTT  >  NC_000913/1720119‑1720390

aCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGcaca                                                                                                                                      <  1:824151/140‑1 (MQ=255)

         tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAgg                                                                                                                             >  2:438360/1‑140 (MQ=255)

         tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAgg                                                                                                                             <  2:278912/140‑1 (MQ=255)

                     gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACaa                                                                                                                               >  2:421644/1‑126 (MQ=255)

                     gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACaa                                                                                                                               <  1:421644/126‑1 (MQ=255)

                             cgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCgg                                                                                                         >  1:78786/1‑140 (MQ=255)

                              gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCggg                                                                                                        <  1:438360/140‑1 (MQ=255)

                              gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCggg                                                                                                        >  2:44501/1‑140 (MQ=255)

                                      aGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtg                                                                                                <  1:217632/140‑1 (MQ=255)

                                       ggTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtgt                                                                                               >  1:718160/1‑140 (MQ=255)

                                         tGGCGTAGCTGGTCAGGGTGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCg                                                                                             <  2:784393/140‑1 (MQ=255)

                                           gCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                                                                                           <  2:449891/140‑1 (MQ=255)

                                           gCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                                                                                           <  1:44501/140‑1 (MQ=255)

                                           gCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                                                                                           <  2:78786/140‑1 (MQ=255)

                                            cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtg                                                                                                <  1:301453/134‑1 (MQ=255)

                                            cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtg                                                                                                >  2:301453/1‑134 (MQ=255)

                                            cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTAc                                                                                          >  1:539419/1‑140 (MQ=255)

                                                   ggTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAg                                                                                   >  2:706517/1‑140 (MQ=255)

                                                    gTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc                                                                                  >  1:800287/1‑140 (MQ=255)

                                                      cAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCaa                                                                                <  2:539419/140‑1 (MQ=255)

                                                                               aaaCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcg                                                       <  2:800287/140‑1 (MQ=255)

                                                                                aaCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgc                                                      <  1:706517/140‑1 (MQ=255)

                                                                                 aCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgcg                                                     <  2:628517/140‑1 (MQ=255)

                                                                                            cGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGtt                                          >  2:540928/1‑140 (MQ=255)

                                                                                                   gAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCgtgt                                   <  2:261027/140‑1 (MQ=255)

                                                                                                       ttCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTc                               <  2:21034/140‑1 (MQ=255)

                                                                                                                    tGGTAATACCCTCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCTCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACca                  <  2:718160/140‑1 (MQ=255)

                                                                                                                        aaTACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACg              <  1:298929/140‑1 (MQ=255)

                                                                                                                                catGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTc      >  1:62838/1‑140 (MQ=255)

                                                                                                                                 atGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGACAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCa     >  1:327014/1‑140 (MQ=255)

                                                                                                                                    gtggcACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCAttt  <  1:297279/140‑1 (MQ=255)

ACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTT  >  NC_000913/1720119‑1720390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A