Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,723,167	C→T	G349G (GGC→GGT)	ydhK →	putative transporter YdhK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,723,167	0	C	T	100.0%	142.6 / NA	41	G349G (GGC→GGT)	ydhK	putative transporter YdhK
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/22); total (19/22)

GTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTA  >  NC_000913/1723033‑1723303

gTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGc                                                                                                                                     <  2:98968/140‑1 (MQ=255)

    ccGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGt                                                                                                                                 >  1:243169/1‑140 (MQ=255)

      gACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCa                                                                                                                               >  1:682668/1‑140 (MQ=255)

      gACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCa                                                                                                                               <  1:210076/140‑1 (MQ=255)

                  gTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGc                                                                                                                   <  2:682668/140‑1 (MQ=255)

                   tCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGcc                                                                                                                  >  1:182245/1‑140 (MQ=255)

                         tCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCt                                                                                                            >  1:58034/1‑140 (MQ=255)

                           tGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTAt                                                                                                          <  1:520495/140‑1 (MQ=255)

                                   aCTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCg                                                                                                  <  2:736084/140‑1 (MQ=255)

                                     tACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCggg                                                                                                <  2:112893/140‑1 (MQ=255)

                                     tACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCggg                                                                                                <  1:648240/140‑1 (MQ=255)

                                          tATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCg                                                                                           <  2:805072/140‑1 (MQ=255)

                                          tATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCg                                                                                           <  2:332784/140‑1 (MQ=255)

                                                 gccgccTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACAttt                                                                                    >  2:13667/1‑140 (MQ=255)

                                                       tttATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACg                                                                              <  1:136828/140‑1 (MQ=255)

                                                               cAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTtgatgat                                                                      >  1:485328/1‑140 (MQ=255)

                                                                 aaGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTtgatgatga                                                                    <  2:58034/140‑1 (MQ=255)

                                                                    tagtCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTg                                                                 >  1:34510/1‑140 (MQ=255)

                                                                      gtCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGgc                                                               >  1:257087/1‑140 (MQ=255)

                                                                      gtCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGgc                                                               <  2:243169/140‑1 (MQ=255)

                                                                         aGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCa                                                            <  1:462054/140‑1 (MQ=255)

                                                                         aGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCa                                                            >  2:577924/1‑140 (MQ=255)

                                                                         aGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACCCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCa                                                            >  1:507341/1‑140 (MQ=255)

                                                                           gAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATg                                                          <  1:577924/140‑1 (MQ=255)

                                                                                      tCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCtt                                               >  1:78467/1‑140 (MQ=255)

                                                                                               cGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATggg                                      <  2:78467/140‑1 (MQ=255)

                                                                                                         gatCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGc                            <  1:13667/140‑1 (MQ=255)

                                                                                                           tCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCa                          <  2:182245/140‑1 (MQ=255)

                                                                                                           tCATACCAGACCCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCa                          <  2:507341/140‑1 (MQ=255)

                                                                                                            cATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                         <  1:68852/140‑1 (MQ=255)

                                                                                                            cATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                         <  1:457/140‑1 (MQ=255)

                                                                                                            cATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                         >  2:674105/1‑140 (MQ=255)

                                                                                                                ccAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCa                     >  2:313793/1‑140 (MQ=255)

                                                                                                                ccAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCa                     >  1:538052/1‑140 (MQ=255)

                                                                                                                    aCTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTaa                 >  1:504322/1‑140 (MQ=255)

                                                                                                                    aCTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTaa                 >  1:440093/1‑140 (MQ=255)

                                                                                                                      tCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACg               >  2:310665/1‑140 (MQ=255)

                                                                                                                      tCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACg               <  1:674105/140‑1 (MQ=255)

                                                                                                                         cACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTg            >  2:342498/1‑140 (MQ=255)

                                                                                                                           cGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgc          >  1:608372/1‑140 (MQ=255)

                                                                                                                                   cGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTa  <  2:34510/140‑1 (MQ=255)

GTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTA  >  NC_000913/1723033‑1723303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A