Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,846,333	C→T	D210N (GAT→AAT)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,846,333	0	C	T	100.0%	160.8 / NA	45	D210N (GAT→AAT)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/24); total (21/24)

GCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCT  >  NC_000913/1846196‑1846466

gcgcCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATtgc                                                                                                                                     >  2:719616/1‑140 (MQ=255)

  gcCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTgcgg                                                                                                                                   >  2:624707/1‑140 (MQ=255)

  gcCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTgcgg                                                                                                                                   >  2:450910/1‑140 (MQ=255)

   cccTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTgcggc                                                                                                                                  >  1:431840/1‑140 (MQ=255)

     cttcttCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGgcgc                                                                                                                                >  1:671176/1‑140 (MQ=255)

          tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  2:562365/140‑1 (MQ=255)

          tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  2:155798/140‑1 (MQ=255)

          tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  1:750337/140‑1 (MQ=255)

          tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  1:624707/140‑1 (MQ=255)

               cTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGcc                                                                                                                      <  2:829510/140‑1 (MQ=255)

                  gTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCaa                                                                                                                   >  2:640041/1‑140 (MQ=255)

                   tACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAg                                                                                                                  >  2:488060/1‑140 (MQ=255)

                        ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  2:559422/1‑140 (MQ=255)

                        ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  1:552620/1‑140 (MQ=255)

                        ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  2:677271/1‑140 (MQ=255)

                        ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  1:133980/1‑140 (MQ=255)

                             acGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTc                                                                                                        >  1:324285/1‑140 (MQ=255)

                                cTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGc                                                                                                     >  2:784298/1‑140 (MQ=255)

                                  tCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCAc                                                                                                   >  1:12684/1‑140 (MQ=255)

                                       cGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTc                                                                                              <  2:12684/140‑1 (MQ=255)

                                        ggTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTcc                                                                                             <  1:70406/140‑1 (MQ=255)

                                           tGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCAc                                                                                          >  1:430463/1‑140 (MQ=255)

                                            gCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACg                                                                                         <  1:577174/140‑1 (MQ=255)

                                             ccAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACgg                                                                                        <  1:719616/140‑1 (MQ=255)

                                                     cGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACg                                                                                <  1:394424/140‑1 (MQ=255)

                                                     cGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACg                                                                                <  1:281830/140‑1 (MQ=255)

                                                     cGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACg                                                                                <  1:230030/140‑1 (MQ=255)

                                                            ccGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCCCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGAt                                                                         <  2:186676/140‑1 (MQ=255)

                                                                          gtgtCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGa                                                           <  2:543565/140‑1 (MQ=255)

                                                                           tgtCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGAc                                                          <  1:450910/140‑1 (MQ=255)

                                                                             tCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCg                                                        >  1:439605/1‑140 (MQ=255)

                                                                                cGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCgaga                                                     <  1:559422/140‑1 (MQ=255)

                                                                                 gATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    <  2:302932/140‑1 (MQ=255)

                                                                                 gATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    <  1:488060/140‑1 (MQ=255)

                                                                                  atatGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAAt                                                   <  2:431840/140‑1 (MQ=255)

                                                                                  atatGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAAt                                                   <  2:133980/140‑1 (MQ=255)

                                                                                     tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    <  1:19554/136‑1 (MQ=255)

                                                                                     tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    >  2:19554/1‑136 (MQ=255)

                                                                                     tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGt                                                >  1:678067/1‑140 (MQ=255)

                                                                                     tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGt                                                <  1:640041/140‑1 (MQ=255)

                                                                                                ttCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGtat                                              >  2:474971/1‑131 (MQ=255)

                                                                                                ttCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGtat                                              <  1:474971/131‑1 (MQ=255)

                                                                                                 tCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGt                                    <  1:677271/140‑1 (MQ=255)

                                                                                                  cAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTc                                   >  1:564259/1‑140 (MQ=255)

                                                                                                            tttCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGc                         <  2:439605/140‑1 (MQ=255)

                                                                                                            tttCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGc                         >  2:307363/1‑140 (MQ=255)

                                                                                                                 ccACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTAc                    >  2:302540/1‑140 (MQ=255)

                                                                                                                                   ctTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCt  >  2:380525/1‑140 (MQ=255)

GCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCT  >  NC_000913/1846196‑1846466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A