Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,849,454	T→C	G206G (GGT→GGC)	sppA →	protease IV, a signal peptide peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,849,454	0	T	C	100.0%	133.2 / NA	37	G206G (GGT→GGC)	sppA	protease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/19); total (18/19)

CCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCC  >  NC_000913/1849324‑1849584

ccAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATaaa                                                                                                                           >  2:362314/1‑140 (MQ=255)

ccAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATaaa                                                                                                                           >  2:709168/1‑140 (MQ=255)

  aGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATc                                                                                                                         <  2:227124/140‑1 (MQ=255)

                      gCTGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTc                                                                                                     <  1:738611/140‑1 (MQ=255)

                      gCTGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTc                                                                                                     <  1:709168/140‑1 (MQ=255)

                        tGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGt                                                                                                   <  1:524446/140‑1 (MQ=255)

                        tGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGt                                                                                                   <  1:127012/140‑1 (MQ=255)

                          tctcCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGtga                                                                                                 <  1:362314/140‑1 (MQ=255)

                          tctcCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGtga                                                                                                 <  1:750217/140‑1 (MQ=255)

                                               cTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCcg                                                                            <  2:130273/140‑1 (MQ=255)

                                                 gCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCcgcg                                                                          >  2:782857/1‑140 (MQ=255)

                                                   aCGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGaa                                                                        >  2:220178/1‑140 (MQ=255)

                                                       cTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCgg                                                                                   >  2:82968/1‑125 (MQ=255)

                                                       cTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCgg                                                                                   <  1:82968/125‑1 (MQ=255)

                                                       cTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCgg                                                                                   <  1:28468/125‑1 (MQ=255)

                                                       cTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCgg                                                                                   >  2:28468/1‑125 (MQ=255)

                                                       cTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTg                                                                    >  2:27827/1‑140 (MQ=255)

                                                         ttGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGAc                                                                  <  1:353641/140‑1 (MQ=255)

                                                                   cGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGa                                                        >  2:181343/1‑140 (MQ=255)

                                                                       cTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTgg                                                    <  1:220178/140‑1 (MQ=255)

                                                                        tGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGt                                                   >  1:701034/1‑140 (MQ=255)

                                                                               cAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCtgt                                            >  2:515000/1‑140 (MQ=255)

                                                                                aaaTCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCtgtg                                           >  1:164267/1‑140 (MQ=255)

                                                                                    cGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTg                                                     <  2:258525/126‑1 (MQ=255)

                                                                                    cGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTg                                                     >  1:258525/1‑126 (MQ=255)

                                                                                    cGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCaa                                       >  2:571669/1‑140 (MQ=255)

                                                                                    cGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCaa                                       >  2:695651/1‑140 (MQ=255)

                                                                                     gTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTAATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCaaa                                      <  1:245931/140‑1 (MQ=255)

                                                                                      ttGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCaaaa                                     <  2:96266/140‑1 (MQ=255)

                                                                                       tGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAAc                                    <  1:425095/140‑1 (MQ=255)

                                                                                       tGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAAc                                    <  1:228517/140‑1 (MQ=255)

                                                                                                aGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAAt                           <  2:701034/140‑1 (MQ=255)

                                                                                                 gCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATa                          >  1:760157/1‑140 (MQ=255)

                                                                                                 gCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATa                          >  1:302056/1‑140 (MQ=255)

                                                                                                 gCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATa                          >  2:652252/1‑140 (MQ=255)

                                                                                                                    gtgtTCCGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCa       <  2:417473/140‑1 (MQ=255)

                                                                                                                         ccGCGTGGGCACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATcc  >  1:528310/1‑140 (MQ=255)

CCAGTTTCGCCAATAAAATTTGGCTGTCTCCGCAAGGCGTGGTTGATCTGCACGGCTTTGCCACCAACGGTCTGTACTACAAATCGTTGCTGGATAAGCTGAAAGTTTCCACCCATGTGTTCCGCGTGGGTACGTATAAATCTGCCGTTGAACCGTTTATTCGTGATGATATGTCACCGGCAGCCCGCGAAGCTGACAGCCGCTGGATTGGTGAGCTGTGGCAAAACTATCTGAATACTGTTGCCGCTAACCGGCAGATCC  >  NC_000913/1849324‑1849584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A