Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 116 49.4 1663006 99.2% 1649701 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,924,381 T→C T71A (ACG→GCG)  yebZ ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,924,3810TC100.0% 69.8 / NA 20T71A (ACG→GCG) yebZputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

CATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGTTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGCACCGTA  >  NC_000913/1924247‑1924516
                                                                                                                                      |                                                                                                                                       
cATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCa                                                                                                                                    <  2:205446/140‑1 (MQ=255)
     tCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAAc                                                                                                                               <  2:392385/140‑1 (MQ=255)
          ggTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGcc                                                                                                                          <  1:292055/140‑1 (MQ=255)
                   aGGGCGACGATCAACGTTACAAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGc                                                                                                                 <  1:167085/140‑1 (MQ=255)
                                        aGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGAt                                                                                            <  1:181710/140‑1 (MQ=255)
                                           gCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCaa                                                                                         >  2:811044/1‑140 (MQ=255)
                                                           aTAACCAGATGCCACCAAACTGCGTTTGC‑‑AACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTg                                                                       <  2:800541/140‑1 (MQ=255)
                                                                   aTGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCtgttg                                                                 >  1:234701/1‑140 (MQ=255)
                                                                       caccaAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTaaa                                                             >  1:279802/1‑140 (MQ=255)
                                                                        accaAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAAc                                                            >  2:416965/1‑140 (MQ=255)
                                                                         ccaAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACg                                                           >  2:293676/1‑140 (MQ=255)
                                                                          caAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGc                                                          >  1:400927/1‑140 (MQ=255)
                                                                            aaCTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAg                                                        >  1:458168/1‑140 (MQ=255)
                                                                                               ccTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAg                                     >  1:481629/1‑140 (MQ=255)
                                                                                                       aTGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAAt                             >  2:124616/1‑140 (MQ=255)
                                                                                                            tGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCa                        <  2:458168/140‑1 (MQ=255)
                                                                                                                 aaaaTACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAgggg                   <  1:293676/140‑1 (MQ=255)
                                                                                                                          cTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCATCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGccagccag          <  2:710250/140‑1 (MQ=255)
                                                                                                                            gTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGc        <  1:274692/140‑1 (MQ=255)
                                                                                                                                  cccGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGCACCGTa  >  1:794302/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGTTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGCACCGTA  >  NC_000913/1924247‑1924516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A