Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,086,480	A→G	S195P (TCT→CCT)	plaP ←	putrescine:H(+) symporter PlaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,086,480	0	A	G	100.0%	135.5 / NA	37	S195P (TCT→CCT)	plaP	putrescine:H(+) symporter PlaP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/20); total (17/20)

AGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATT  >  NC_000913/2086344‑2086614

aGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGccaggcca                                                                                                                                     <  1:327/140‑1 (MQ=255)

aGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGccaggcca                                                                                                                                     <  1:798433/140‑1 (MQ=255)

        cGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGAc                                                                                                                             <  2:609490/140‑1 (MQ=255)

          cACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGa                                                                                                                           >  1:330402/1‑140 (MQ=255)

                ggATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTc                                                                                                                     <  2:726748/140‑1 (MQ=255)

                ggATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTc                                                                                                                     <  1:419318/140‑1 (MQ=255)

                       gcgcTCTTCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCg                                                                                                              >  1:1654/1‑140 (MQ=255)

                                   tttGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGcctt                                                                                                  <  1:457269/140‑1 (MQ=255)

                                     tGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGGGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGccttca                                                                                                >  1:445538/1‑140 (MQ=255)

                                         ttcttcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGccttcacctt                                                                                            >  2:727568/1‑140 (MQ=255)

                                            ttcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCaa                                                                                         >  1:181780/1‑140 (MQ=255)

                                             tcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCaaa                                                                                        <  1:185888/140‑1 (MQ=255)

                                                 aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:705421/140‑1 (MQ=255)

                                                 aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    >  2:433741/1‑140 (MQ=255)

                                                 aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:642474/140‑1 (MQ=255)

                                                 aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:309656/140‑1 (MQ=255)

                                                     gTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCAt                                                                                >  1:628344/1‑140 (MQ=255)

                                                        gCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATaaa                                                                             <  2:1654/140‑1 (MQ=255)

                                                              gCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:143585/127‑1 (MQ=255)

                                                              gCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    >  2:143585/1‑127 (MQ=255)

                                                                cGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATg                                                                     >  1:447324/1‑140 (MQ=255)

                                                                  tCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGcc                                                                   <  1:433741/140‑1 (MQ=255)

                                                                            tAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCAcc                                                         <  1:543881/140‑1 (MQ=255)

                                                                               aGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACcgcg                                                      <  1:607063/140‑1 (MQ=255)

                                                                                ggAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGa                                                     <  1:25948/140‑1 (MQ=255)

                                                                                ggAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGa                                                     <  1:727568/140‑1 (MQ=255)

                                                                                 gagaAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGAt                                                    <  2:330402/140‑1 (MQ=255)

                                                                                  agaAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGACTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATc                                                   >  1:659066/1‑140 (MQ=255)

                                                                                    aaGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAg                                                 <  1:416121/140‑1 (MQ=255)

                                                                                            gATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGACTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGcggtgatt                                         <  2:659066/140‑8 (MQ=255)

                                                                                              ttGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGAAGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGc                                       <  2:234873/140‑1 (MQ=255)

                                                                                                 tCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAac                                    >  2:390316/1‑140 (MQ=255)

                                                                                                 tCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAac                                    >  2:705197/1‑140 (MQ=255)

                                                                                                   gCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAacac                                  <  1:25491/140‑1 (MQ=255)

                                                                                                             tcatcGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACa                        <  2:445538/140‑1 (MQ=255)

                                                                                                                   ggATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTg                  >  2:653703/1‑140 (MQ=255)

                                                                                                                           tGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGc          >  2:373391/1‑140 (MQ=255)

                                                                                                                              gcATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTAc       >  2:694387/1‑140 (MQ=255)

                                                                                                                               cATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACg      >  1:21833/1‑140 (MQ=255)

                                                                                                                                  cGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGAt   >  2:415804/1‑140 (MQ=255)

                                                                                                                                   gccaggccagAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGAtt  <  2:181780/140‑1 (MQ=255)

                                                                                                                                   gccaggccagAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGAtt  >  2:76415/1‑140 (MQ=255)

AGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATT  >  NC_000913/2086344‑2086614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A