Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,307,508	T→C	T370A (ACG→GCG)	yojI ←	ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,307,508	0	T	C	100.0%	135.2 / NA	37	T370A (ACG→GCG)	yojI	ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/20); total (17/20)

CGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAG  >  NC_000913/2307371‑2307643

cGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCca                                                                                                                                       >  1:729063/1‑140 (MQ=255)

 ggCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCcaa                                                                                                                                      >  2:184399/1‑140 (MQ=255)

        gcagTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATc                                                                                                                               >  2:780665/1‑140 (MQ=255)

            ttGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCa                                                                                                                           >  2:95252/1‑140 (MQ=255)

                     cAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGAtt                                                                                                                  <  2:584528/140‑1 (MQ=255)

                      aGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGAttt                                                                                                                 >  2:73397/1‑140 (MQ=255)

                                          tGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                                                                                             <  1:184399/140‑1 (MQ=255)

                                          tGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                                                                                             >  2:812203/1‑140 (MQ=255)

                                          tGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                                                                                             >  2:347212/1‑140 (MQ=255)

                                           gCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgc                                                                                                >  2:413476/1‑136 (MQ=255)

                                           gCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgc                                                                                                <  1:413476/136‑1 (MQ=255)

                                                 aaCAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGaaa                                                                                      >  2:560861/1‑140 (MQ=255)

                                                       ttGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAAcagcag                                                                                <  1:780665/140‑1 (MQ=255)

                                                             tAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGcc                                                                          <  2:162904/140‑1 (MQ=255)

                                                             tAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGcc                                                                          <  1:4211/140‑1 (MQ=255)

                                                                tCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACg                                                                       >  2:169798/1‑140 (MQ=255)

                                                                     cGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGa                                                                  <  1:229634/140‑1 (MQ=255)

                                                                       gttgttCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATg                                                                >  1:579152/1‑140 (MQ=255)

                                                                       gttgttCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATg                                                                >  1:165611/1‑140 (MQ=255)

                                                                        ttgttCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATgg                                                               <  1:560861/140‑1 (MQ=255)

                                                                            tCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTgag                                                           >  1:106223/1‑140 (MQ=255)

                                                                                 gcTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTaa                                                      >  2:117002/1‑140 (MQ=255)

                                                                                      cAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGa                                                 <  1:117002/140‑1 (MQ=255)

                                                                                                   cAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcg                                    >  2:415519/1‑140 (MQ=255)

                                                                                                     gCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCTAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcgcg                                  <  1:812203/140‑1 (MQ=255)

                                                                                                     gCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcgcg                                  <  2:16593/140‑1 (MQ=255)

                                                                                                     gCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcgcg                                  <  1:415519/140‑1 (MQ=255)

                                                                                                       aaGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGtt                                <  2:165611/140‑1 (MQ=255)

                                                                                                        aGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTa                               <  2:234737/140‑1 (MQ=255)

                                                                                                          aTTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTCGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATc                             <  1:169798/140‑1 (MQ=255)

                                                                                                                        tgGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCATCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACg               <  2:729063/140‑1 (MQ=255)

                                                                                                                        tgGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACg               <  2:106223/140‑1 (MQ=255)

                                                                                                                          gCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTc             <  1:739309/140‑1 (MQ=255)

                                                                                                                             gATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACg          >  2:438753/1‑140 (MQ=255)

                                                                                                                                  aaGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACg     <  1:703939/140‑1 (MQ=255)

                                                                                                                                  aaGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACg     <  1:438753/140‑1 (MQ=255)

                                                                                                                                     cccGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAg  >  1:615126/1‑140 (MQ=255)

CGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAG  >  NC_000913/2307371‑2307643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A