Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,867,700	T→C	T352A (ACC→GCC)	nlpD ←	murein hydrolase activator NlpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,867,700	0	T	C	100.0%	176.2 / NA	52	T352A (ACC→GCC)	nlpD	murein hydrolase activator NlpD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/32); total (20/32)

TGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAG  >  NC_000913/2867569‑2867835

tGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCa                                                                                                                                 >  2:295377/1‑140 (MQ=255)

 gATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCAt                                                                                                                                >  1:396500/1‑140 (MQ=255)

 gATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCAt                                                                                                                                >  2:193572/1‑140 (MQ=255)

  aTCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATg                                                                                                                               <  2:757707/140‑1 (MQ=255)

     ccTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGt                                                                                                                             >  1:781359/1‑139 (MQ=255)

     ccTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGt                                                                                                                             <  2:781359/139‑1 (MQ=255)

       ttGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGc                                                                                                                          >  2:336222/1‑140 (MQ=255)

        tGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACNGGNTCCGGCNCTACCCATGGTCGCt                                                                                                                         <  1:582643/140‑1 (MQ=255)

          aCGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTAt                                                                                                                       >  1:695070/1‑140 (MQ=255)

             gAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTAtttt                                                                                                                    >  2:380358/1‑140 (MQ=255)

              aaCATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTAttttt                                                                                                                   >  2:162385/1‑140 (MQ=255)

                   tCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTACCGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGccc                                                                                                              <  2:728289/140‑1 (MQ=255)

                   tCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGccc                                                                                                              <  2:497206/140‑1 (MQ=255)

                   tCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGccc                                                                                                              <  2:396500/140‑1 (MQ=255)

                   tCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGccc                                                                                                              <  1:623652/140‑1 (MQ=255)

                   tCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGccc                                                                                                              <  2:828808/140‑1 (MQ=255)

                   tCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGcc                                                                                                               >  1:499227/1‑139 (MQ=255)

                   tCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGcc                                                                                                               <  2:499227/139‑1 (MQ=255)

                       gCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGcc                                                                                                          <  2:332212/140‑1 (MQ=255)

                        cAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCGGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCt                                                                                                         >  1:728032/1‑140 (MQ=255)

                        cAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCt                                                                                                         >  2:459700/1‑140 (MQ=255)

                           aaGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTaa                                                                                                      >  2:794075/1‑140 (MQ=255)

                                  gTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCttg                                                                                               <  1:465644/140‑1 (MQ=255)

                                   ttCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCttgt                                                                                              >  1:806127/1‑140 (MQ=255)

                                   ttCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCttgt                                                                                              <  2:259389/140‑1 (MQ=255)

                                    tCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCttgtt                                                                                             <  2:485032/140‑1 (MQ=255)

                                       gccgATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTgttgtt                                                                                          >  1:625512/1‑140 (MQ=255)

                                       gccgATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTgttgtt                                                                                          >  2:363310/1‑140 (MQ=255)

                                             ttATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGa                                                                                    <  2:625512/140‑1 (MQ=255)

                                             ttATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGa                                                                                    <  1:363310/140‑1 (MQ=255)

                                              tATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGAc                                                                                   <  1:336222/140‑1 (MQ=255)

                                                   cTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGTTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCa                                                                              <  2:206482/140‑1 (MQ=255)

                                                   cTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCa                                                                              <  2:806127/140‑1 (MQ=255)

                                                      cGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATtg                                                                           >  1:710671/1‑140 (MQ=255)

                                                              aaCGCGGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                   <  2:728032/140‑1 (MQ=255)

                                                              aaCGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                   <  1:459700/140‑1 (MQ=255)

                                                                cGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATg                                                                 >  2:538584/1‑140 (MQ=255)

                                                                     cGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt                                                            <  1:295377/140‑1 (MQ=255)

                                                                     cGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt                                                            <  1:818504/140‑1 (MQ=255)

                                                                            aCGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACt                                                     <  2:710671/140‑1 (MQ=255)

                                                                             cGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTc                                                    <  2:695070/140‑1 (MQ=255)

                                                                             cGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTc                                                    <  1:193572/140‑1 (MQ=255)

                                                                             cGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTc                                                    <  2:373927/140‑1 (MQ=255)

                                                                                 tttCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt                                                <  1:538584/140‑1 (MQ=255)

                                                                                   tCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa                                              >  2:69263/1‑140 (MQ=255)

                                                                                    ccccTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAat                                             <  1:794075/140‑1 (MQ=255)

                                                                                     cccTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatc                                            <  1:313303/140‑1 (MQ=255)

                                                                                           tAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCAtt                                      <  1:828343/140‑1 (MQ=255)

                                                                                                     cAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatg                            <  2:166401/140‑1 (MQ=255)

                                                                                                     cAAAATGCAAGCGTGTTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatg                            >  2:812585/1‑140 (MQ=255)

                                                                                                                  tgtTGAACTGGTTCCGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTAc               <  1:162385/140‑1 (MQ=255)

                                                                                                                               ccGGCGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg  >  1:822109/1‑140 (MQ=255)

TGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAG  >  NC_000913/2867569‑2867835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A