Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,911	C→T	A368A (GCG→GCA)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,911	0	C	T	100.0%	68.5 / NA	21	A368A (GCG→GCA)	aas	acyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (9/12); total (9/12)

CCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGC  >  NC_000913/2974781‑2975046

ccGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCtcttct                                                                                                                                >  1:65362/1‑140 (MQ=255)

       aaaGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGgct                                                                                                                         <  2:624646/140‑1 (MQ=255)

             aTCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGtt                                                                                                                   <  2:97046/140‑1 (MQ=255)

                 ttGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAAc                                                                                                               <  1:111077/140‑1 (MQ=255)

                 ttGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAAc                                                                                                               <  2:193322/140‑1 (MQ=255)

                       gtgAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGc                                                                                                         >  1:163159/1‑140 (MQ=255)

                        tgAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGcc                                                                                                        >  1:192849/1‑140 (MQ=255)

                                    tCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTAAGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATc                                                                                            >  1:16875/1‑140 (MQ=255)

                                           aaTCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAAt                                                                                     <  2:712914/140‑1 (MQ=255)

                                                gCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCg                                                                                <  2:108905/140‑1 (MQ=255)

                                                gCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTACCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCg                                                                                <  1:581540/140‑1 (MQ=255)

                                                  tCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGaa                                                                              <  1:303899/140‑1 (MQ=255)

                                                     aCATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAt                                                                           <  2:371387/140‑1 (MQ=255)

                                                                                       cgCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCtttt                                         >  2:450983/1‑140 (MQ=255)

                                                                                         ccTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaa                                       <  2:300001/140‑1 (MQ=255)

                                                                                                      cTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAg                          <  1:450983/140‑1 (MQ=255)

                                                                                                       ttCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGa                         <  2:784360/140‑1 (MQ=255)

                                                                                                               cGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAgcgc                 >  2:203841/1‑140 (MQ=255)

                                                                                                                 gAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCAc               >  1:216217/1‑140 (MQ=255)

                                                                                                                            gATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGtt    >  1:754526/1‑140 (MQ=255)

                                                                                                                              tCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGc  >  1:407337/1‑140 (MQ=255)

CCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGC  >  NC_000913/2974781‑2975046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A