Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,180,822	G→A	W134* (TGG→TGA)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,822	0	G	A	100.0%	155.6 / NA	45	W134* (TGG→TGA)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/17); total (28/17)

CATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACCTGCTGT  >  NC_000913/3180686‑3180957

cATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGGTCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCATCGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctgactg                                                                                                                                      <  2:510304/140‑1 (MQ=255)

 aTGGCTGACGCACCAGCCATCGTTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctgactgg                                                                                                                                     <  1:586675/140‑1 (MQ=255)

      tGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGAt                                                                                                                                <  2:612599/140‑1 (MQ=255)

        aCGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCa                                                                                                                              >  1:66452/1‑140 (MQ=255)

         cgtaCCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAg                                                                                                                             >  1:542444/4‑140 (MQ=255)

              cAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTaa                                                                                                                        <  1:691494/140‑1 (MQ=255)

                                        tCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCg                                                                                              <  2:745264/140‑1 (MQ=255)

                                        tCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCg                                                                                              <  2:260892/140‑1 (MQ=255)

                                          tGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGAc                                                                                            <  2:542444/140‑1 (MQ=255)

                                            gCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCa                                                                                          <  1:477/140‑1 (MQ=255)

                                             cGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTg                                                                                                           >  2:796350/1‑122 (MQ=255)

                                             cGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTg                                                                                                           <  1:796350/122‑1 (MQ=255)

                                              gTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGt                                                                                        >  2:399250/1‑140 (MQ=255)

                                                      gCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGt                                                                                >  1:61655/1‑140 (MQ=255)

                                                        aCTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCt                                                                              <  2:696711/140‑1 (MQ=255)

                                                            gTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCaa                                                                          >  1:65658/1‑140 (MQ=255)

                                                            gTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCaa                                                                          >  1:6177/1‑140 (MQ=255)

                                                                    aaaCTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACt                                                                  >  2:686735/1‑140 (MQ=255)

                                                                      aCTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTga                                                                >  2:782095/1‑140 (MQ=255)

                                                                      aCTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTga                                                                >  1:737747/1‑140 (MQ=255)

                                                                             gAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGatcgatcg                                                         <  2:367336/140‑1 (MQ=255)

                                                                               aaaTAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGatcgatcg                                                         >  1:444777/1‑138 (MQ=255)

                                                                               aaaTAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGatcgatcg                                                         <  2:444777/138‑1 (MQ=255)

                                                                                  tAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCg                                                    >  2:605872/1‑140 (MQ=255)

                                                                                    aCGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGtt                                                  >  1:250221/1‑140 (MQ=255)

                                                                                      gCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGa                                                <  1:609832/140‑1 (MQ=255)

                                                                                       ccGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAg                                               <  2:250221/140‑1 (MQ=255)

                                                                                          aTACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTg                                            >  1:263234/1‑140 (MQ=255)

                                                                                                     tCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGc                                              <  2:126809/127‑1 (MQ=255)

                                                                                                     tCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGc                                              >  1:126809/1‑127 (MQ=255)

                                                                                                       aCTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATg                               >  2:447746/1‑140 (MQ=255)

                                                                                                                ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      >  2:151462/1‑140 (MQ=255)

                                                                                                                ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      >  2:575995/1‑140 (MQ=255)

                                                                                                                ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      <  2:6177/140‑1 (MQ=255)

                                                                                                                ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      >  1:726173/1‑140 (MQ=255)

                                                                                                                ggcggcGTTCTTTCAGGGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGAAGCTACCAGTTTAACAGTCTGCAAGAAAAACTGATCTATCGCTTCGTTGACCTGCGTGAAAAGTATCGCTTCCAGt                      >  2:590128/1‑140 (MQ=255)

                                                                                                                 gcggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGtt                     >  2:124727/1‑140 (MQ=255)

                                                                                                                  cggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtg                    <  2:65658/140‑1 (MQ=255)

                                                                                                                  cggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtg                    <  2:726173/140‑1 (MQ=255)

                                                                                                                   ggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTTACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGAACGCTTCGGTGAGCAGCGTGAACAGTATGGCTTCCAGTtgc                   >  2:733093/1‑140 (MQ=255)

                                                                                                                   ggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgc                   >  1:18469/1‑140 (MQ=255)

                                                                                                                   ggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGACTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgc                   >  2:453922/1‑140 (MQ=255)

                                                                                                                     cgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgctg                 >  2:661415/1‑140 (MQ=255)

                                                                                                                     cgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgctg                 >  1:685796/1‑140 (MQ=255)

                                                                                                                            tCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCa          >  2:86460/1‑140 (MQ=255)

                                                                                                                                   aTCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACctgctg   <  2:685796/140‑1 (MQ=255)

                                                                                                                                    tCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACCTGCTGt  >  1:406236/1‑140 (MQ=255)

CATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACCTGCTGT  >  NC_000913/3180686‑3180957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A