Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R1
|
116 |
49.4 |
1663006 |
99.2% |
1649701 |
139.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,728,110 |
T→C |
D421G (GAT→GGT) |
xylB ← |
xylulokinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,728,110 | 0 | T | C | 80.7%
| 84.5
/ 15.7
| 31 | coding (1262/1455 nt) | xylB | xylulokinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (12/13); minor base . (3/3); total (15/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 |
CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATG > NC_000913/3727974‑3728244
|
cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAcccc < 1:422846/140‑1 (MQ=255)
aTAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACcccccc < 1:191165/140‑1 (MQ=255)
cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTa < 2:404541/140‑1 (MQ=255)
cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTCGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTa < 1:69792/140‑1 (MQ=255)
gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACg > 1:133525/1‑140 (MQ=255)
aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAAt > 1:526855/1‑140 (MQ=255)
cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACCGTAATCGAGCT‑GCTGACCGCTGatat > 1:649805/1‑140 (MQ=255)
cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑CCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATc > 1:828456/1‑140 (MQ=255)
cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCCTACGGTAATCGAGCT‑GCTGACCGCTGATATCCg > 1:818368/1‑140 (MQ=255)
ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCTTACGGAAATCGACCT‑CCTGACCCCTGATATCCGCCa > 1:376900/1‑140 (MQ=255)
ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑CCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAg > 1:266738/1‑140 (MQ=255)
aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACg < 1:784768/140‑1 (MQ=255)
aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCCAGCT‑GCTGACCGCCGATCTCTGCCCGCATCTGCCg > 2:658373/1‑140 (MQ=255)
aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGGACGGTAATCGAACT‑GCTGACCGCCGCTATCCGCCAGCATCTTAcc > 2:306591/1‑139 (MQ=255)
cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑CCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa > 2:144570/1‑140 (MQ=255)
ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa > 1:771468/1‑140 (MQ=255)
caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt < 1:512150/140‑1 (MQ=255)
acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa < 1:230125/140‑1 (MQ=255)
gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccc > 1:612859/1‑139 (MQ=255)
gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc > 1:88734/1‑140 (MQ=255)
aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc < 2:82050/140‑1 (MQ=255)
aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccccccc > 2:499113/1‑140 (MQ=255)
ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑CCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt < 2:828456/140‑1 (MQ=255)
tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCCCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCa < 2:133525/140‑1 (MQ=255)
gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑CCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:144570/140‑1 (MQ=255)
aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg < 1:658373/140‑1 (MQ=255)
tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg < 2:215781/140‑1 (MQ=255)
ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑CCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa < 2:266738/140‑1 (MQ=255)
aGCCTTGCTGCGCCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt > 2:810152/1‑140 (MQ=255)
gctgcGCCCAGTGCTGGCCCCACACCCCCCCCCAAAAAGAAGCCCAGCTCTCTTA‑TACTCATATGCCCCTGCCTCCGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCgaa > 2:561144/1‑138 (MQ=255)
cgcCCAGTGCTGGCCCCACCCCCCCCCCCATACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa < 1:76199/140‑1 (MQ=255)
gcCCAGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt < 1:527084/140‑1 (MQ=255)
aGTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc > 2:512085/1‑140 (MQ=255)
gTGCTGGCCCCACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg < 2:612859/140‑1 (MQ=255)
ccACACCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATg < 2:88734/140‑1 (MQ=255)
|
CTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCT‑GCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATG > NC_000913/3727974‑3728244
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
