Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R1
|
116 |
49.4 |
1663006 |
99.2% |
1649701 |
139.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,762,260 |
G→A |
V26V (GTG→GTA) |
rhsA → |
rhs element protein RhsA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,762,260 | 0 | G | A | 100.0%
| 22.7
/ NA
| 11 | V26V (GTG→GTA) | rhsA | rhs element protein RhsA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/6); total (5/6) |
TGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCC > NC_000913/3762130‑3762325
|
tGTAGGGTTTAGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTTTTTAGGGGTCAGCCGGGGGACGTATTGGt > 2:394489/1‑140 (MQ=14)
tAGGGTTTAGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTTAGGGGTCAGCCGGGGGACGTATTGGTGc > 2:284548/1‑140 (MQ=14)
tttCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCa < 2:89694/140‑1 (MQ=255)
tttCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGGACGTATTGGTGCCCCCa > 2:267850/1‑140 (MQ=255)
tttCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGCCGGGGGACGTATTGGTGcccccc > 2:317087/1‑139 (MQ=255)
ccTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGCCGGGGGACGTATTGGTGCCCCCACCCGc > 1:3324/1‑140 (MQ=14)
gTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTg > 2:46205/1‑140 (MQ=255)
tCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTgg > 1:598319/1‑140 (MQ=255)
aCCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGc < 2:598319/140‑1 (MQ=255)
ccGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGccc < 1:558785/140‑1 (MQ=255)
aTAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGGTCAGCCGGGGGACGTATTGGTGCCCCCCCCGGCGTGGCCTGTTCGGTGTGGCCcgg > 1:572479/1‑140 (MQ=14)
gATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACg < 1:808247/140‑1 (MQ=255)
tGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTc < 1:711531/140‑1 (MQ=21)
agaAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCg < 2:300868/140‑1 (MQ=18)
aGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCTGGGCTCAGCCGGGGCACGCATTGGGGCCCCCCCCGGCGTGGCCCGTTCGGTGTGCCCCCGCGGAGGGGCCGCCGGcccccc > 2:652222/1‑136 (MQ=11)
|
TGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCC > NC_000913/3762130‑3762325
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
