Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 143 98.3 3330986 99.3% 3307669 138.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,793,268 A→C H97Q (CAT→CAG)  btuD ← vitamin B12 ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,793,2680AC100.0% 271.2 / NA 76H97Q (CAT→CAG) btuDvitamin B12 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (37/39);  total (37/39)

TGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTAC  >  NC_000913/1793131‑1793404
                                                                                                                                         |                                                                                                                                        
tGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGctgctg                                                                                                                                        >  1:1507350/1‑140 (MQ=255)
  cAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCa                                                                                                                                      <  1:248830/140‑1 (MQ=255)
  cAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCa                                                                                                                                      <  1:377867/140‑1 (MQ=255)
  cAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCa                                                                                                                                      >  2:273913/1‑140 (MQ=255)
     caccacCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAgtg                                                                                                                                   >  2:273302/1‑140 (MQ=255)
       ccaccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTc                                                                                                                                 >  2:232133/1‑140 (MQ=255)
       ccaccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATAGTGCTGCTGCAGTGTc                                                                                                                                 >  1:1076190/1‑140 (MQ=255)
        caccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCa                                                                                                                                >  1:728821/1‑140 (MQ=255)
          ccGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGctgctg                                                                                                                                        >  1:381847/1‑130 (MQ=255)
           cGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGt                                                                                                                             >  2:1415683/1‑140 (MQ=255)
            gcagcaAGACGTACGCGTTGCCATTCGCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTa                                                                                                                            <  1:84904/140‑1 (MQ=255)
                caAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGc                                                                                                                        >  2:1405317/1‑140 (MQ=255)
                  aGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGctgctg                                                                                                                                        >  1:29272/1‑122 (MQ=255)
                  aGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCa                                                                                                                      >  2:1317034/1‑140 (MQ=255)
                  aGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCa                                                                                                                      >  1:233901/1‑140 (MQ=255)
                     cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   >  1:1624144/1‑140 (MQ=255)
                     cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   <  1:264797/140‑1 (MQ=255)
                     cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   >  1:639093/1‑140 (MQ=255)
                     cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   <  2:639093/140‑1 (MQ=255)
                     cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   >  1:1421763/1‑140 (MQ=255)
                     cGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGAc                                                                                                                   <  2:728821/140‑1 (MQ=255)
                        aCGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCgg                                                                                                                <  1:732829/140‑1 (MQ=255)
                        aCGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCgg                                                                                                                >  1:1408907/1‑140 (MQ=255)
                          gcgTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATAGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCg                                                                                                              <  2:1076190/140‑1 (MQ=255)
                             ttGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTg                                                                                                           >  2:290152/1‑140 (MQ=255)
                             ttGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTg                                                                                                           >  2:1621624/1‑140 (MQ=255)
                              tGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGc                                                                                                          >  2:1315601/1‑140 (MQ=255)
                               gCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGCACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCa                                                                                                         >  1:1505656/1‑140 (MQ=255)
                                      aCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                                                                  <  1:273302/140‑1 (MQ=255)
                                      aCCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAAcggcg                                                                                                  <  2:1214413/140‑1 (MQ=255)
                                        cgccgGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACggcggc                                                                                                >  1:1542967/1‑140 (MQ=255)
                                        cgccgGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACggcggc                                                                                                >  1:1312078/1‑140 (MQ=255)
                                          ccgGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGTGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGt                                                                                              >  1:427276/1‑140 (MQ=255)
                                            ggAAAGTTGATTGGTGCTTCGTCCGAGTTTGTCATCAAGAGGCAGCGCCCCTGCGCCATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTct                                                                                            <  1:1180496/140‑1 (MQ=255)
                                               aaGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgct                                                                                         <  1:1405317/140‑1 (MQ=255)
                                                aGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                                                                        <  2:1507350/140‑1 (MQ=255)
                                                aGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTctgctg                                                                                        <  2:1288625/140‑1 (MQ=255)
                                                     atcggTGCTACGTCCGAGTTTGTCATCAAGCGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTtgtg                                                                                   <  2:1019095/137‑1 (MQ=255)
                                                      ttGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGa                                                                                  <  1:1415683/140‑1 (MQ=255)
                                                          tGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGa                                                                              >  1:1158746/1‑140 (MQ=255)
                                                           gCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGAt                                                                             <  2:1154510/140‑1 (MQ=255)
                                                               cGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgc                                                                         >  1:836830/1‑140 (MQ=255)
                                                                gTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGgcg                                                                        <  2:836830/140‑1 (MQ=255)
                                                                    gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                                                                     <  1:1062540/123‑1 (MQ=255)
                                                                    gAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCtgttg                                                                                     >  2:1062540/1‑123 (MQ=255)
                                                                         tGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               >  2:1013850/1‑140 (MQ=255)
                                                                         tGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGCACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCGGACCGGCGTTGCAAACGGCGGCGTCCGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               <  2:1505656/140‑1 (MQ=255)
                                                                           tcatcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAATGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgcgc                                                             >  2:1512889/1‑140 (MQ=255)
                                                                            catcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGcgcg                                                            <  1:1512889/140‑1 (MQ=255)
                                                                              tcaAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAg                                                          <  2:1542967/140‑1 (MQ=255)
                                                                                      cAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTttgttg                                                  <  2:1155405/140‑1 (MQ=255)
                                                                                       aGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGc                                                 >  2:763951/1‑140 (MQ=255)
                                                                                       aGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGc                                                 >  1:777934/1‑140 (MQ=255)
                                                                                         cgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               >  2:325275/1‑124 (MQ=255)
                                                                                         cgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAgc                                                               <  1:325275/124‑1 (MQ=255)
                                                                                         cgcCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCgg                                               >  1:23786/1‑140 (MQ=255)
                                                                                           ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGAc                                             <  2:233901/140‑1 (MQ=255)
                                                                                               tGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATg                                         <  2:1408907/140‑1 (MQ=255)
                                                                                                           cAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGtt                             <  2:922041/140‑1 (MQ=255)
                                                                                                           cAGTAGTTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGtt                             <  2:1238970/140‑1 (MQ=255)
                                                                                                              tagtTCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGcc                          >  1:995400/1‑140 (MQ=255)
                                                                                                                 ttCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcg                       >  2:947365/1‑140 (MQ=255)
                                                                                                                 ttCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcg                       <  1:947365/140‑1 (MQ=255)
                                                                                                                 ttCGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcg                       <  2:1312078/140‑1 (MQ=255)
                                                                                                                   cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:495915/1‑140 (MQ=255)
                                                                                                                   cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:487018/1‑140 (MQ=255)
                                                                                                                   cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:543375/1‑140 (MQ=255)
                                                                                                                   cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  2:833380/1‑140 (MQ=255)
                                                                                                                   cGGTACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCcgcg                     >  1:1221780/1‑140 (MQ=255)
                                                                                                                      tACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAAc                  <  1:290152/140‑1 (MQ=255)
                                                                                                                      tACGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAAc                  <  2:543375/140‑1 (MQ=255)
                                                                                                                       aCGCGTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTCTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACt                 <  2:1158746/140‑1 (MQ=255)
                                                                                                                        cgcgTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTg                >  2:113899/1‑140 (MQ=255)
                                                                                                                          cgTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGaa              <  1:763951/140‑1 (MQ=255)
                                                                                                                          cgTTTTATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGaa              <  1:1013850/140‑1 (MQ=255)
                                                                                                                            ttttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATg            <  2:1221780/140‑1 (MQ=255)
                                                                                                                            ttttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATg            <  2:495915/140‑1 (MQ=255)
                                                                                                                              ttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCt          <  2:1114774/140‑1 (MQ=255)
                                                                                                                              ttATCGTGCTGCTGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCt          <  2:1421763/140‑1 (MQ=255)
                                                                                                                                     gctgctGCAGTGTCAGGT‑GTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTAc  >  2:350005/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTAC  >  NC_000913/1793131‑1793404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A