Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	143	98.3	3330986	99.3%	3307669	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,601,156	T→C	T311A (ACG→GCG)	ftsX ←	cell division protein FtsX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,601,156	0	T	C	100.0%	122.3 / NA	35	T311A (ACG→GCG)	ftsX	cell division protein FtsX
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/4); total (31/4)

GCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAAT  >  NC_000913/3601021‑3601289

gCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCg                                                                                                                                    <  2:393584/140‑1 (MQ=255)

gCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCg                                                                                                                                    >  1:241476/1‑140 (MQ=255)

gCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCg                                                                                                                                    >  2:1455217/1‑140 (MQ=255)

  ttttATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGaa                                                                                                                                  >  2:301075/1‑140 (MQ=255)

         attCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCtgt                                                                                                                          <  1:886152/140‑1 (MQ=255)

          ttCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCtgtg                                                                                                                         <  2:814709/140‑1 (MQ=255)

            cAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGcc                                                                                                                       <  1:301075/140‑1 (MQ=255)

            cAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGcc                                                                                                                       >  1:1662992/1‑140 (MQ=255)

                    aaGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGc                                                                                                               >  2:224605/1‑140 (MQ=255)

                         gCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAAccg                                                                                                          >  1:1346504/1‑140 (MQ=255)

                          cGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAAccgc                                                                                                         >  1:356361/1‑140 (MQ=255)

                                       cggggccAACCCACGCTCCCCCCCACCCAATCATCGAGCATACCAGCAGAAATAGCAGACATTCATCAAATAATAAGCCATTGATATAAAACTCCCCCCAAAAAC‑CCTGCCCCCCTTCCGCAACCGCCGATGACAATCgc                                                                                            <  2:1329378/134‑1 (MQ=255)

                                          tGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCgcagc                                                                                         >  2:330253/1‑140 (MQ=255)

                                            gCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCAc                                                                                       >  1:1329378/1‑140 (MQ=255)

                                                               gccaaaTCACCGAGCAAACCAGAAAAAAGAGCAGGCATTCATAAAATAATAAGCTATTAATAACAAACTCCCCCCAAAAGC‑CCTGCCCCCCTCCCAAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAAtt                                                                    <  2:871381/136‑1 (MQ=255)

                                                                           aGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAGA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGAcaacaac                                                        >  1:871381/1‑139 (MQ=255)

                                                                              aTACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcg                                                     >  1:652911/1‑140 (MQ=255)

                                                                               tACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcgc                                                    >  1:1048514/1‑140 (MQ=255)

                                                                               tACCAGAAAAAAGAGCAGTCATTCAGCAAATAATAATCAATTAATAT‑AAACCTCCCCCCAAAAACCCTGCCCCCCCCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcgc                                                    <  2:465662/140‑1 (MQ=255)

                                                                                  cagcagCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAg                                                 >  1:1330653/1‑140 (MQ=255)

                                                                                   agcagcAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGa                                                >  1:535798/1‑140 (MQ=255)

                                                                                      agcaATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGaaaa                                             >  1:844735/1‑140 (MQ=255)

                                                                                          aTAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATccc                                         >  1:177597/1‑140 (MQ=255)

                                                                                          aTAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATccc                                         >  1:1632403/1‑140 (MQ=255)

                                                                                               aGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCAcagcag                                    >  1:1523582/1‑140 (MQ=255)

                                                                                                ggCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGt                                   >  1:718194/1‑140 (MQ=255)

                                                                                                 gCATTCATCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTTACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTg                                  >  2:1545229/1‑140 (MQ=255)

                                                                                                      cATCGAATAATAAGCTATTAATATAAACCTCCGCCCCGAAAC‑CCTGTCCCCCTCCCACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcca                             <  1:696562/140‑1 (MQ=255)

                                                                                                      cATCAAATGATAACCTTTTAATATCAAACTCCGCCCCAAAAC‑CCTGCCCCCCTCCCACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcca                             <  2:835267/140‑1 (MQ=255)

                                                                                                       aTCGAATGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGccac                            >  1:835267/1‑140 (MQ=255)

                                                                                                           aaTGATAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATa                        >  1:1065989/1‑140 (MQ=255)

                                                                                                               aTAAGCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAgg                    >  1:803300/1‑140 (MQ=255)

                                                                                                                   gCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACg                >  1:805908/1‑140 (MQ=255)

                                                                                                                   gCCATTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACg                >  2:450296/1‑140 (MQ=255)

                                                                                                                      aTTGATATCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAActgtc             >  1:1363696/1‑136 (MQ=255)

                                                                                                                             tCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGAt      >  2:215105/1‑140 (MQ=255)

                                                                                                                             tCAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGAt      >  2:532800/1‑140 (MQ=255)

                                                                                                                              cAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATg     >  2:1435717/1‑140 (MQ=255)

                                                                                                                              cAAACTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATg     >  1:1186221/1‑140 (MQ=255)

                                                                                                                                 aCTTCGCTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGaac  >  1:1360318/1‑139 (MQ=255)

GCTTTTATTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAAT  >  NC_000913/3601021‑3601289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A