Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,095,988	T→C	F49L (TTC→CTC)	hisF →	imidazole glycerol phosphate synthase subunit HisF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,095,988	0	T	C	100.0%	192.3 / NA	54	F49L (TTC→CTC)	hisF	imidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/23); total (31/23)

AAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCT  >  NC_000913/2095853‑2096121

aaaCGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTa                                                                                                                                   <  1:32674/140‑1 (MQ=255)

    gCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGat                                                                                                                               <  1:751950/140‑1 (MQ=255)

       tAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATc                                                                                                                            >  2:579853/1‑140 (MQ=255)

         aTCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCAc                                                                                                                          <  1:78549/140‑1 (MQ=255)

             cATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCt                                                                                                                      >  2:180530/1‑140 (MQ=255)

              aTGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCtt                                                                                                                     <  1:78573/140‑1 (MQ=255)

               tGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTc                                                                                                                    >  2:809773/1‑140 (MQ=255)

                gTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTcc                                                                                                                   >  2:152998/1‑140 (MQ=255)

                    cGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCg                                                                                                               >  1:454137/1‑140 (MQ=255)

                    cGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCg                                                                                                               >  1:1160226/1‑140 (MQ=255)

                     gACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGa                                                                                                              >  2:234168/1‑140 (MQ=255)

                        gTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATgg                                                                                                           <  1:579853/140‑1 (MQ=255)

                          tCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGcc                                                                                                         <  1:144870/140‑1 (MQ=255)

                          tCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGACAAAACGCCACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGcc                                                                                                         >  1:251329/1‑140 (MQ=255)

                                tGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTgg                                                                                                   <  1:784456/140‑1 (MQ=255)

                                tGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTgg                                                                                                   <  2:307525/140‑1 (MQ=255)

                                    cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCgtg                                                                                                      <  1:129352/133‑1 (MQ=255)

                                    cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCgtg                                                                                                      >  2:129352/1‑133 (MQ=255)

                                    cAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGa                                                                                               >  2:252483/1‑140 (MQ=255)

                                     aGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGAt                                                                                              <  1:185901/140‑1 (MQ=255)

                                     aGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGAt                                                                                              <  1:131516/140‑1 (MQ=255)

                                      ggtggtGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATa                                                                                             >  1:1182619/1‑140 (MQ=255)

                                         ggtgAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATaaaa                                                                                          >  2:92131/1‑140 (MQ=255)

                                             aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTg                                                                                      >  1:263196/1‑140 (MQ=255)

                                                 gCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTa                                                                                  >  1:9970/1‑140 (MQ=255)

                                                  cGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAt                                                                                 >  1:633686/1‑140 (MQ=255)

                                                    tACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAtct                                                                               >  1:553089/1‑140 (MQ=255)

                                                    tACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAtct                                                                               >  2:386265/1‑140 (MQ=255)

                                                    tACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTAtct                                                                               >  1:24979/1‑140 (MQ=255)

                                                      cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTACAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTcg                                                                             <  1:526854/140‑1 (MQ=255)

                                                        gTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTcgcg                                                                           <  1:386265/140‑1 (MQ=255)

                                                        gTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTcgcg                                                                           <  2:454137/140‑1 (MQ=255)

                                                           tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTgg                                                                        <  2:633686/140‑1 (MQ=255)

                                                           tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTgg                                                                        >  1:1162552/1‑140 (MQ=255)

                                                              cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCgg                                                                     <  1:1082791/140‑1 (MQ=255)

                                                              cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCgg                                                                     <  1:234168/140‑1 (MQ=255)

                                                                aCCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGaa                                                                   <  2:24979/140‑1 (MQ=255)

                                                                 ccATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAg                                                                  >  2:622961/1‑140 (MQ=255)

                                                                        aTCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGa                                                           >  1:580653/1‑140 (MQ=255)

                                                                           aTTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACAt                                                        >  1:470901/1‑140 (MQ=255)

                                                                              ggCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTcc                                                     >  2:332352/1‑140 (MQ=255)

                                                                                             cTGGCAAAATGCTACGCTGAAGAAAGCGCTGACGAAATGGTGCTATACGATATGACCGCTTCCAGCGATAGCAGTGAGGTAGATAAAAACTCGGTATCTCGCGTGGCCGAAGGGAGCGATATTCCGTTTTGTGTGGCgag                                      >  2:1207822/1‑138 (MQ=255)

                                                                                             cTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGTggg                                      <  2:263196/140‑1 (MQ=255)

                                                                                             cTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCggg                                      >  2:955974/1‑140 (MQ=255)

                                                                                              tGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGt                                     <  2:982550/140‑1 (MQ=255)

                                                                                              tGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGt                                     <  1:206071/140‑1 (MQ=255)

                                                                                               ggCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTg                                    >  1:593850/1‑140 (MQ=255)

                                                                                                    aaCGCTACGCTGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGAtt                               <  2:1182619/140‑1 (MQ=255)

                                                                                                     acgctacgctGAAGAAGGCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTTGGGTTa                              >  1:226016/1‑140 (MQ=255)

                                                                                                                     ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTGGGAGATGc              >  2:719119/1‑140 (MQ=255)

                                                                                                                     ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGGCTCTGGAAGATGc              >  2:546575/1‑140 (MQ=255)

                                                                                                                     ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGATTAAGTCTCTGGAAGATGc              >  1:365874/1‑140 (MQ=255)

                                                                                                                     ggCGCTGACGAACTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGAGGCGGGTGGGATTAAGTCTCTGGAAGATGc              >  1:1019140/1‑140 (MQ=255)

                                                                                                                                 cTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAATCTCTGGAAGATGCCGCGAAAAATCt  >  2:961548/1‑140 (MQ=255)

                                                                                                                                 cTGGTGCTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCt  <  1:180530/140‑1 (MQ=255)

AAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCT  >  NC_000913/2095853‑2096121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A