Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,419	G→A	Q127* (CAA→TAA)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,419	0	G	A	97.9%	153.9 / ‑4.3	48	Q127* (CAA→TAA)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (27/20); total (27/21)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

AAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTC  >  NC_000913/2329283‑2329547

aaGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTAccc                                                                                                                               >  1:273077/1‑140 (MQ=255)

     ttCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCcaca                                                                                                                          >  2:407406/1‑140 (MQ=255)

       ccTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCcacaca                                                                                                                        >  2:157541/1‑140 (MQ=255)

       ccTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCaacaca                                                                                                                        >  1:68338/1‑140 (MQ=255)

            gCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTgc                                                                                                                   <  2:273077/140‑1 (MQ=255)

                tGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCa                                                                                                               >  2:1078640/1‑140 (MQ=255)

                tGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCa                                                                                                               <  1:531338/140‑1 (MQ=255)

                       cgcgGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCa                                                                                                        >  1:536546/1‑140 (MQ=255)

                           ggTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGcc                                                                                                                <  2:517663/128‑1 (MQ=255)

                           ggTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGcc                                                                                                                >  1:517663/1‑128 (MQ=255)

                                cGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTgcg                                                                                               <  1:864315/140‑1 (MQ=255)

                                cGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTgcg                                                                                               <  1:696498/140‑1 (MQ=255)

                                 gTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTgcgg                                                                                              >  2:282585/1‑140 (MQ=255)

                                     cTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCa                                                                                          <  2:719876/140‑1 (MQ=255)

                                      tAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCaa                                                                                         >  2:1187663/1‑140 (MQ=255)

                                            aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga                                                                                   <  2:299980/140‑1 (MQ=255)

                                            aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga                                                                                   <  1:608726/140‑1 (MQ=255)

                                            aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga                                                                                   <  1:1187663/140‑1 (MQ=255)

                                            aTCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATaga                                                                                   <  2:536546/140‑1 (MQ=255)

                                             tCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTg                                                                                                 >  1:430283/1‑125 (MQ=255)

                                             tCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTg                                                                                                 <  2:430283/125‑1 (MQ=255)

                                             tCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATagag                                                                                  >  2:328647/1‑140 (MQ=255)

                                                  cagcGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCcac                                                                             <  2:570823/140‑1 (MQ=255)

                                                       aTTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGaa                                                                        >  2:1937/1‑140 (MQ=255)

                                                            aCCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTAc                                                                   <  1:1189853/140‑1 (MQ=255)

                                                                  aCCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAgcgc                                                             >  2:332571/1‑140 (MQ=255)

                                                                      aGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGTTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTa                                                         >  1:645101/1‑140 (MQ=255)

                                                                       ggTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTAt                                                        >  1:1183473/1‑140 (MQ=255)

                                                                            cTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATcgcgc                                                   >  2:314847/1‑140 (MQ=255)

                                                                                  tCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATg                                             >  2:456762/1‑140 (MQ=255)

                                                                                    aGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGct                                           >  2:903613/1‑140 (MQ=255)

                                                                                     gCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGctc                                          >  1:945930/1‑140 (MQ=255)

                                                                                     gCGTAGTCAGAGACGCTAACCACGCAGCTGCCTGACGCTGTTGCCAGCATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGctc                                          >  2:509700/1‑140 (MQ=255)

                                                                                        tAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTcc                                       <  2:1183473/140‑1 (MQ=255)

                                                                                         aGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGct                                           <  2:1161114/135‑1 (MQ=255)

                                                                                         aGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGct                                           >  1:1161114/1‑135 (MQ=255)

                                                                                         aGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTccc                                      <  1:314847/140‑1 (MQ=255)

                                                                                                 aCGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGa                              >  2:133614/1‑140 (MQ=255)

                                                                                                  cGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac                             >  1:288834/1‑140 (MQ=255)

                                                                                                  cGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac                             <  1:1110518/140‑1 (MQ=255)

                                                                                                     tAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGacaac                          >  1:114608/1‑140 (MQ=255)

                                                                                                      aaCCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGacaaca                         >  2:20228/1‑140 (MQ=255)

                                                                                                       aCCACGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCAACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGAcaacaa                        <  2:68338/140‑1 (MQ=255)

                                                                                                           cGCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATaaa                    >  2:880088/1‑140 (MQ=255)

                                                                                                            gCAGGTGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAAc                   <  1:407406/140‑1 (MQ=255)

                                                                                                                 tGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac                             >  1:933920/1‑125 (MQ=255)

                                                                                                                 tGCCTGACGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGac                             <  2:933920/125‑1 (MQ=255)

                                                                                                                        cGCTGTTGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGa       >  2:707829/1‑140 (MQ=255)

                                                                                                                             ttGCCAGTATTACCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTc  <  2:936120/140‑1 (MQ=255)

AAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGGCGCAAAATAGAGCCCACAGGAACGTACCAGCGCGGTATCGCGCGTTATGCTCTCCCCAGCCTGACAACAATAAACCTCTTCCGGGGAGTTTC  >  NC_000913/2329283‑2329547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A