Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,436,339	G→A	P104L (CCC→CTC)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,339	0	G	A	100.0%	165.5 / NA	48	P104L (CCC→CTC)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/19); total (29/19)

AACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCG  >  NC_000913/2436202‑2436473

aaCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGt                                                                                                                                      >  1:488495/1‑140 (MQ=255)

 aCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGtt                                                                                                                                     >  1:879309/1‑140 (MQ=255)

 aCCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGtt                                                                                                                                     >  1:266921/1‑140 (MQ=255)

   cGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCg                                                                                                                                   >  1:689637/1‑140 (MQ=255)

     cccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCgag                                                                                                                                 <  2:488495/140‑1 (MQ=255)

     cccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCgag                                                                                                                                 >  2:382569/1‑140 (MQ=255)

     cccTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCgag                                                                                                                                 >  2:200140/1‑140 (MQ=255)

       cTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCg                                                                                                                                   >  1:95395/1‑136 (MQ=255)

       cTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCg                                                                                                                                   <  2:95395/136‑1 (MQ=255)

        tGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGc                                                                                                                              >  1:663577/1‑140 (MQ=255)

                tAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATc                                                                                                                      <  2:15818/140‑1 (MQ=255)

                tAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATc                                                                                                                      <  1:200140/140‑1 (MQ=255)

                   cGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCAc                                                                                                                   <  2:590363/140‑1 (MQ=255)

                    ggTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCAct                                                                                                                  >  1:175070/1‑140 (MQ=255)

                        tCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCActgctg                                                                                                              >  2:121882/1‑140 (MQ=255)

                        tCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCActgctg                                                                                                              >  2:488136/1‑140 (MQ=255)

                         cAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGt                                                                                                             >  1:1028832/1‑140 (MQ=255)

                            tGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGa                                                                                                          >  1:266729/1‑140 (MQ=255)

                                gccAGCAGCTGGCT‑GTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCAcc                                                                                                     >  2:32017/1‑140 (MQ=255)

                                   agcagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAg                                                                                                   <  2:1028832/140‑1 (MQ=255)

                                    gcagcTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAgg                                                                                                  <  2:266729/140‑1 (MQ=255)

                                       gctggctgGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCaa                                                                                               <  1:179858/140‑1 (MQ=255)

                                                    aCTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCg                                                                                  <  1:488136/140‑1 (MQ=255)

                                                         cTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGcttct                                                                             >  1:912901/1‑140 (MQ=255)

                                                         cTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGcttct                                                                             <  2:879309/140‑1 (MQ=255)

                                                             tACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCaa                                                                         >  1:653828/1‑140 (MQ=255)

                                                                ggCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAAccc                                                                      >  1:1128400/1‑140 (MQ=255)

                                                                  cGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAg                                                                    >  2:764628/1‑140 (MQ=255)

                                                                   gATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAgg                                                                   <  2:663577/140‑1 (MQ=255)

                                                                    aTGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGc                                                                  >  2:354382/1‑140 (MQ=255)

                                                                           tccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCAGCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTa                                                           <  1:455225/140‑1 (MQ=255)

                                                                           tccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTa                                                           >  1:644599/1‑140 (MQ=255)

                                                                           tccggttccggtAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTa                                                           <  2:912901/140‑1 (MQ=255)

                                                                                            gTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGAc                                          >  2:219034/1‑140 (MQ=255)

                                                                                                      aCGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGcc                                <  1:32017/140‑1 (MQ=255)

                                                                                                       cGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCa                               >  2:799194/1‑140 (MQ=255)

                                                                                                       cGGGTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCa                               >  1:345517/1‑140 (MQ=255)

                                                                                                        gggTTTACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAg                              <  1:382569/140‑1 (MQ=255)

                                                                                                           tttACTTCCGGCACCACCAGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGTCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTg                           >  2:567202/1‑140 (MQ=255)

                                                                                                                         caccaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGGGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGt             >  1:1048343/1‑140 (MQ=255)

                                                                                                                          accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAg              >  2:1038046/1‑138 (MQ=255)

                                                                                                                          accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAg              <  1:1038046/138‑1 (MQ=255)

                                                                                                                          accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTc            >  2:344244/1‑140 (MQ=255)

                                                                                                                          accaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTc            >  2:825307/1‑140 (MQ=255)

                                                                                                                           ccaGCGGTACGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCg           <  2:266921/140‑1 (MQ=255)

                                                                                                                                    cGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg  <  1:825307/140‑1 (MQ=255)

                                                                                                                                    cGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg  >  1:669248/1‑140 (MQ=255)

                                                                                                                                    cGTCGAGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCg  <  1:219034/140‑1 (MQ=255)

AACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGGTACGGCGATGACATTCCGGTTCCGGTAATCTGTCAGTACAAACGGGTTTACTTCCGGCACCACCAGCGGTACGTCGGGTTCGAGAGCAAACAATCCACTGCTGTCGATCACCAGGCAACCTGAGTTGGTCGCTTCTTCAACCCAGGCAGCGGTAGCTTCTTTGCCTGCGACAAAAAATGCCAGCTGCGCCTGCGTCCAGTCGAATTCAGCG  >  NC_000913/2436202‑2436473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A