Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,889	(C)_9→10	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,880	1	.	C	80.7%	171.2 / 35.7	62	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (6/6); new base C (19/31); total (25/37)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCA  >  NC_000913/2462743‑2463012

ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑cc                                                                                                                                     >  2:729144/1‑140 (MQ=255)

ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑cc                                                                                                                                     >  2:1144025/1‑140 (MQ=255)

ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑cc                                                                                                                                     <  1:675897/140‑1 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑cc‑ccccccc                                                                                                                             <  2:494235/140‑1 (MQ=255)

          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑cc‑cccccccctg                                                                                                                          >  1:249479/1‑137 (MQ=255)

              ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTa                                                                                                                       <  2:999498/140‑1 (MQ=255)

              ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTa                                                                                                                       <  2:460541/140‑1 (MQ=255)

                 gCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATtg                                                                                                                    <  2:540817/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATtgt                                                                                                                   >  1:23764/1‑140 (MQ=255)

                   aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTCCCC‑CCCCCCCTGGCTATtgt                                                                                                                   >  2:1023631/1‑140 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTgcg                                                                                                                >  2:51606/1‑140 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCCGCATTTCCAGGA‑TACTCCCC‑CCCCCCCTGGCTATtgtg                                                                                                                  <  1:1043000/140‑1 (MQ=255)

                     cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTgcg                                                                                                                <  1:1062423/140‑1 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCGGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTc                                                                                                             >  1:145362/1‑140 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTAGCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTc                                                                                                             >  2:503242/1‑140 (MQ=255)

                         cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCa                                                                                                            <  2:1203302/140‑1 (MQ=255)

                          ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATa                                                                                                          <  2:230775/140‑1 (MQ=255)

                                       tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                               >  1:779700/1‑139 (MQ=255)

                                       tAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                               <  2:779700/139‑1 (MQ=255)

                                           cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  1:1185930/140‑1 (MQ=255)

                                              tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          >  2:294790/1‑137 (MQ=255)

                                              tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  1:294790/137‑1 (MQ=255)

                                                tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  1:17251/140‑1 (MQ=255)

                                                 ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTc                                                                                    >  1:457051/1‑140 (MQ=255)

                                                 ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCt                                                                                   <  1:51606/140‑1 (MQ=255)

                                                     aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                >  1:670964/1‑140 (MQ=255)

                                                     aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                               >  2:280341/1‑140 (MQ=255)

                                                      gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTTCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGGGCGCTCATACACTCAAATTAAAAATAGGTTCtaaaa                                                                               >  2:542118/1‑139 (MQ=255)

                                                        cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataa                                                                             >  2:379461/1‑140 (MQ=255)

                                                           aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                          >  1:529381/1‑140 (MQ=255)

                                                           aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑‑C‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                        >  2:67485/1‑140 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaa                                                                                 >  2:1214880/1‑132 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaa                                                                                 <  1:1214880/132‑1 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                        >  1:803371/1‑140 (MQ=255)

                                                                 aGGGAAAGGCATATTTTCAGGCGTTCTGCTGGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                                                                    >  1:326138/1‑140 (MQ=255)

                                                                 aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                                                                    <  2:249479/140‑1 (MQ=255)

                                                                  gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                                                                   <  2:66299/140‑1 (MQ=255)

                                                                   ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttttt                                                                 >  2:979148/1‑140 (MQ=255)

                                                                   ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttttt                                                                 <  2:803371/140‑1 (MQ=255)

                                                                     aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑‑C‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:67485/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  2:529381/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:493441/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  2:457051/140‑1 (MQ=255)

                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTCCCC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:1023631/140‑1 (MQ=255)

                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                       <  1:979148/140‑1 (MQ=255)

                                                                              ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                       <  1:729144/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                  <  2:396687/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                  <  2:130541/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                 <  2:5862/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  1:1144025/140‑1 (MQ=255)

                                                                                     gCGTTCTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  1:503242/140‑1 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTTCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                             <  1:542118/140‑1 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCTTTAgg                                             >  1:70832/1‑140 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                 <  1:53227/136‑1 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                 >  2:53227/1‑136 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                             >  2:538822/1‑140 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                             <  2:23764/140‑1 (MQ=255)

                                                                                        ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                             <  2:423003/140‑1 (MQ=255)

                                                                                          cTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTTATTAGGTa                                           <  1:348762/140‑1 (MQ=255)

                                                                                              tCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                       <  1:336123/140‑1 (MQ=255)

                                                                                               cGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGTTAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata                                      >  1:659891/1‑140 (MQ=255)

                                                                                                            gagTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaaa                         <  2:98433/140‑1 (MQ=255)

                                                                                                             agTTTACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaat                        >  1:386161/1‑140 (MQ=255)

                                                                                                                ctACTTTTCTGCATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaa                     >  1:335732/2‑140 (MQ=255)

                                                                                                                 tACTTTTTTGCATTTCCAGGA‑TACT‑‑CCACCCCCCATGGCTGTTGTGCGTTCACACCCTCAAATTAAAGGTAGTTTCCAAAAAAAAGAGCGTTTTTTGAAAGACTATTTAATAAAAAAACATAAATTTCTAAAAaatcaat                    >  2:539448/1‑140 (MQ=255)

                                                                                                                      ttCTGCATTTCCAGGACGCCC‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACTCTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa               <  1:634841/140‑1 (MQ=255)

                                                                                                                           cATTTCCAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGaa          <  2:1145982/140‑1 (MQ=255)

                                                                                                                                ccAGGA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATaaaa     >  1:1167467/1‑140 (MQ=255)

                                                                                                                                   ggA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCa  <  2:508480/140‑1 (MQ=255)

                                                                                                                                   ggA‑TACTC‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCa  <  2:386161/140‑1 (MQ=255)

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGA‑TACT‑‑CC‑CCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCA  >  NC_000913/2462743‑2463012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A