Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 228 65.7 2434974 91.7% 2232871 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,554,937 A→G G82G (GGT→GGC)  eutR ← putative AraC‑type transcriptional regulator EutR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,554,9370AG100.0% 149.9 / NA 43G82G (GGT→GGC) eutRputative AraC‑type transcriptional regulator EutR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

GTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTG  >  NC_000913/2554803‑2555073
                                                                                                                                      |                                                                                                                                        
gTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGgt                                                                                                                                     >  2:792616/1‑140 (MQ=255)
     accaGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTAtt                                                                                                                                <  1:792616/140‑1 (MQ=255)
     accaGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTAtt                                                                                                                                <  1:421734/140‑1 (MQ=255)
      ccaGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTc                                                                                                                               >  1:257206/1‑140 (MQ=255)
       caGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCa                                                                                                                              >  1:582104/1‑140 (MQ=255)
            cGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGaaa                                                                                                                         <  2:257206/140‑1 (MQ=255)
               gTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAAc                                                                                                                      <  2:119482/140‑1 (MQ=255)
                        tCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATatc                                                                                                             <  1:578131/140‑1 (MQ=255)
                         ccGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATatca                                                                                                            <  2:627428/140‑1 (MQ=255)
                                         ttGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATGTCATCGAGTAGAATTTCCg                                                                                            >  1:264015/1‑140 (MQ=255)
                                           gCGAACCGATAAATCCCTGTTCGCCGCGCGTCGCCGGATTACCATACCAGAACAAGTTCGGCCGGACAAAGCACGATTTACACAGCGCCAGGCCGGTGTACTTACGATAAACCTGAATATCAACGAGTATAATTTCCGTa                                                                                          >  2:695887/1‑140 (MQ=255)
                                           gCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTa                                                                                          >  2:853753/1‑140 (MQ=255)
                                           gCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTa                                                                                          >  2:241220/1‑140 (MQ=255)
                                               aCCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAAtt                                                                                      <  2:1170816/140‑1 (MQ=255)
                                                  gATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGGGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCAc                                                                                   >  1:684056/1‑140 (MQ=255)
                                                    tAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCa                                                                                 >  2:504410/1‑140 (MQ=255)
                                                           cTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACt                                                                          <  1:1150382/140‑1 (MQ=255)
                                                             gCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTg                                                                        >  1:1138506/1‑140 (MQ=255)
                                                              cTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAAtt                                                                                      >  1:211123/1‑125 (MQ=255)
                                                              cTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAAtt                                                                                      <  2:211123/125‑1 (MQ=255)
                                                              cTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGc                                                                       >  1:533686/1‑140 (MQ=255)
                                                                cACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGccc                                                                     <  1:1073927/140‑1 (MQ=255)
                                                                  ccGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGGATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCgg                                                                   <  2:1131468/140‑1 (MQ=255)
                                                                   cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCaa                                                                                   <  2:72255/123‑2 (MQ=255)
                                                                   cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCAc                                                                                   >  1:72255/1‑123 (MQ=255)
                                                                   cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGcc                                                                      <  2:684051/136‑1 (MQ=255)
                                                                   cgcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGcc                                                                      >  1:684051/1‑136 (MQ=255)
                                                                    gcgcgTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGAt                                                                 >  2:758120/1‑140 (MQ=255)
                                                                        gTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAg                                                             >  2:171072/1‑140 (MQ=255)
                                                                                aaTGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGAt                                                                 <  1:994729/128‑1 (MQ=255)
                                                                                aaTGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGAt                                                                 >  2:994729/1‑128 (MQ=255)
                                                                                       aaCCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATAATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATctg                                              <  1:1205877/140‑1 (MQ=255)
                                                                                           aGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCtgctgc                                          <  1:171072/140‑1 (MQ=255)
                                                                                               cGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAgg                                      <  1:241220/140‑1 (MQ=255)
                                                                                                   ttCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGt                                  >  1:926362/1‑140 (MQ=255)
                                                                                                   ttCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGt                                  <  2:1138506/140‑1 (MQ=255)
                                                                                                    tCGGCCAGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTa                                 <  2:328134/140‑1 (MQ=255)
                                                                                                          aGACCAGGCACGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATGTCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGtt                           <  2:264015/140‑1 (MQ=255)
                                                                                                                    cGACTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGtt                 <  2:645391/140‑1 (MQ=255)
                                                                                                                      aCTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCa               >  1:1064295/1‑140 (MQ=255)
                                                                                                                       cTGACGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCAt              >  1:1028207/1‑140 (MQ=255)
                                                                                                                          aCGCAGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGa           >  2:217037/1‑140 (MQ=255)
                                                                                                                              aGCGCCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATc       >  1:471812/1‑140 (MQ=255)
                                                                                                                                cgcCAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCg     >  1:1101994/1‑140 (MQ=255)
                                                                                                                                   cAGGCCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTtg  <  1:758120/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
GTGCCACCAGGGCGGGTGGCGATTTCCGCGCTTCCCAGACATTGCGAACCGATAAATCCCTGCTCACCGCGCGTCGCCGGAATGCCAAACCAGAACGAGTTCGGCCAGACCAGGCACGACTGACGCAGCGCCAGACCGGTGTATTCACGAAAAACCTGAATATCATCGAGTAGAATTTCCGTAAATTCACCATGAAACTTGCCCGGATGCAGCTGATCGTAAATCTGCTGCCAGGCGGTAATCGTTAAAGCATGTTCATAGACATCCGTTG  >  NC_000913/2554803‑2555073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A