Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,480	Δ1 bp	coding (815/951 nt)	talA →	transaldolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,478	0	C	.	100.0%	244.0 / NA	58	coding (813/951 nt)	talA	transaldolase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (31/27); total (31/27)

GCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACT  >  NC_000913/2579339‑2579612

gCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGc                                                                                                                                        <  2:266567/140‑1 (MQ=255)

gCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGc                                                                                                                                        <  2:502001/140‑1 (MQ=255)

     cGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGccagct                                                                                                                                   <  1:119099/140‑5 (MQ=255)

     cGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGccagct                                                                                                                                   <  2:287993/140‑5 (MQ=255)

               aaCAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCg                                                                                                                        >  1:950065/1‑140 (MQ=255)

               aaCAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCg                                                                                                                        >  2:581058/1‑140 (MQ=255)

               aaCAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCg                                                                                                                        <  2:648311/140‑1 (MQ=255)

                      ccTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGa                                                                                                                 <  1:964890/140‑1 (MQ=255)

                         cGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGtt                                                                                                              >  2:652169/1‑140 (MQ=255)

                          gCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTc                                                                                                             >  1:1137103/1‑140 (MQ=255)

                                   ggCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg                                                                                                    <  1:1015662/140‑1 (MQ=255)

                                   ggCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg                                                                                                    <  2:239053/140‑1 (MQ=255)

                                   ggCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg                                                                                                    <  1:939608/140‑1 (MQ=255)

                                   ggCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg                                                                                                    <  1:65861/140‑1 (MQ=255)

                                   ggCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAg                                                                                                    <  1:542684/140‑1 (MQ=255)

                                               ctgactATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGAt                                                                                        >  1:1198635/1‑140 (MQ=255)

                                                  actATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCg                                                                                     >  2:661375/1‑140 (MQ=255)

                                                  actATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCg                                                                                     <  2:1145510/140‑1 (MQ=255)

                                                  actATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCg                                                                                     >  2:703683/1‑140 (MQ=255)

                                                   ctATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGCTGGGAGCACAATCAGGATGCGa                                                                                    >  1:858083/1‑140 (MQ=255)

                                                     aTCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATg                                                                                  >  2:1040378/1‑140 (MQ=255)

                                                       cGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGc                                                                                >  2:472471/1‑140 (MQ=255)

                                                       cGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGc                                                                                <  1:661375/140‑1 (MQ=255)

                                                         cACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCgg                                                                              <  2:1198635/140‑1 (MQ=255)

                                                         cACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCgg                                                                              >  1:675016/1‑140 (MQ=255)

                                                               aTTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGaaa                                                                        <  1:189493/140‑1 (MQ=255)

                                                                 ttACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGaaaaa                                                                      <  1:652169/140‑1 (MQ=255)

                                                                      gAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTc                                                                 <  1:960742/140‑1 (MQ=255)

                                                                           aGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAg                                                            >  2:123240/1‑140 (MQ=255)

                                                                             cTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGa                                                                          <  1:70872/124‑1 (MQ=255)

                                                                             cTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGa                                                                          >  2:70872/1‑124 (MQ=255)

                                                                              tGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCa                                                         <  1:1173029/140‑1 (MQ=255)

                                                                              tGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCa                                                         <  2:858083/140‑1 (MQ=255)

                                                                              tGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCa                                                         >  1:237586/1‑140 (MQ=255)

                                                                                cAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCAtt                                                       <  1:629736/140‑1 (MQ=255)

                                                                                 aGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTc                                                      >  1:944702/1‑140 (MQ=255)

                                                                                      aaaGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTg                                                 >  1:137838/1‑140 (MQ=255)

                                                                                       aaGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGt                                                >  2:176326/1‑140 (MQ=255)

                                                                                             cGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCg                                          <  1:1040378/140‑1 (MQ=255)

                                                                                                 aGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGa                                      <  1:1081888/140‑1 (MQ=255)

                                                                                                       aCGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACg                                <  2:88162/140‑1 (MQ=255)

                                                                                                       aCGTAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACg                                <  2:675016/140‑1 (MQ=255)

                                                                                                          tAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCaa                             >  1:531786/1‑140 (MQ=255)

                                                                                                          tAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCa                              <  1:505142/139‑1 (MQ=255)

                                                                                                          tAAATTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCa                              >  2:505142/1‑139 (MQ=255)

                                                                                                             aTTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACt                          <  2:855726/140‑1 (MQ=255)

                                                                                                             aTTAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACt                          >  1:1115746/1‑140 (MQ=255)

                                                                                                               tAATCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTgg                        <  2:531786/140‑1 (MQ=255)

                                                                                                                aaTCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGa                       >  2:720106/1‑140 (MQ=255)

                                                                                                                 aTCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGaa                      >  2:51582/1‑140 (MQ=255)

                                                                                                                 aTCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGaa                      <  1:123240/140‑1 (MQ=255)

                                                                                                                 aTCCCACCTTCTCAGACGTTCCCA‑GTCTAGCTCGCATGAGCGAAGCGGGGTTCCGTTGGGAGCACAATCGGGATGCGTTGGCGGGAGATAGACTGGCTTGAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGaa                      >  2:720659/1‑140 (MQ=255)

                                                                                                                  tCCCACCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAg                     >  2:736551/1‑140 (MQ=255)

                                                                                                                      aCCTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtct                 >  1:500321/1‑140 (MQ=255)

                                                                                                                       ccTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtctt                >  2:1104449/1‑140 (MQ=255)

                                                                                                                       ccTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtctt                >  1:327212/1‑140 (MQ=255)

                                                                                                                       ccTTCTCAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGAtctt                >  2:459493/1‑140 (MQ=255)

                                                                                                                           ctcAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTg            >  1:270528/1‑140 (MQ=255)

                                                                                                                             cAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTgcc          >  2:896113/1‑140 (MQ=255)

                                                                                                                             cAGACGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCCTgtc          >  1:337051/1‑138 (MQ=255)

                                                                                                                                 cGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCa      <  1:703683/140‑1 (MQ=255)

                                                                                                                                 cGTTCCCACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGAACAACGCAAACTGGAAGATCTTCTTGCCGCCa      <  2:671965/140‑1 (MQ=255)

                                                                                                                                     cccACG‑CCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACt  >  1:139360/1‑140 (MQ=255)

GCTTCCGTCGCACCGAACAAATCCTCGCCTTAACCGGCTGCGATCGACTGACTATCGCACCGAATTTACTGAAGGAGCTGCAGGAAAAAGTTTCGCCAGTGGTACGTAAATTAATCCCACCTTCTCAGACGTTCCCACGCCCAGCTCCCATGAGCGAAGCGGAGTTCCGTTGGGAGCACAATCAGGATGCGATGGCGGTAGAAAAACTGTCTGAAGGCATTCGTCTGTTCGCCGTTGATCAACGCAAACTGGAAGATCTTCTTGCCGCCAAACT  >  NC_000913/2579339‑2579612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A