Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 228 65.7 2434974 91.7% 2232871 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,854,583 T→C G82G (GGT→GGC)  fhlA → DNA‑binding transcriptional activator FhlA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,854,5830TC100.0% 196.0 / NA 55G82G (GGT→GGC) fhlADNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

CTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG  >  NC_000913/2854459‑2854718
                                                                                                                            |                                                                                                                                       
cTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCAtt                                                                                                                          >  2:350515/1‑140 (MQ=255)
     cTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGt                                                                                                                      >  2:267142/1‑139 (MQ=255)
     cTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGt                                                                                                                      <  1:267142/139‑1 (MQ=255)
        gCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTcgcg                                                                                                                  <  2:474242/140‑1 (MQ=255)
          cGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGcc                                                                                                                <  2:501323/140‑1 (MQ=255)
             cAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTg                                                                                                             >  2:444159/1‑140 (MQ=255)
                cgcgGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCCCACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATa                                                                                                          <  2:310836/140‑1 (MQ=255)
                  cgGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACg                                                                                                        <  2:425368/140‑1 (MQ=255)
                       aTTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCa                                                                                                   <  1:350515/140‑1 (MQ=255)
                               gtgGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGtt                                                                                           >  2:1141282/1‑140 (MQ=255)
                                  gCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACg                                                                                        >  2:513072/1‑140 (MQ=255)
                                  gCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACg                                                                                        >  2:63537/1‑140 (MQ=255)
                                   cAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACga                                                                                       <  1:959530/140‑1 (MQ=255)
                                     aGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACgaag                                                                                     >  1:313116/1‑140 (MQ=255)
                                           gACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAAtttt                                                                               >  2:392825/1‑140 (MQ=255)
                                                 aCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTtgtg                                                                            <  1:93228/137‑1 (MQ=255)
                                                 aCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTtgtg                                                                            >  2:93228/1‑137 (MQ=255)
                                                 aCGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGaaa                                                                         >  1:400490/1‑140 (MQ=255)
                                                           tattaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCa                                                               >  2:1203585/1‑140 (MQ=255)
                                                            attaCGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAg                                                              <  1:1141282/140‑1 (MQ=255)
                                                                 gcgtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGa                                                         <  1:63537/140‑1 (MQ=255)
                                                                   gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACg                                                       >  1:871489/1‑140 (MQ=255)
                                                                   gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACg                                                       >  2:477133/1‑140 (MQ=255)
                                                                   gtcgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACg                                                       >  1:14572/1‑140 (MQ=255)
                                                                     cgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCa                                                     >  1:823369/1‑140 (MQ=255)
                                                                     cgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCa                                                     >  2:289984/1‑140 (MQ=255)
                                                                     cgcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCa                                                     >  1:952992/1‑140 (MQ=255)
                                                                      gcgtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAg                                                    <  2:14572/140‑1 (MQ=255)
                                                                        gtGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGt                                                  <  2:952992/140‑1 (MQ=255)
                                                                             aaaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCt                                             <  2:355088/140‑1 (MQ=255)
                                                                              aaGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCta                                            <  1:423076/140‑1 (MQ=255)
                                                                               aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGc                                                      <  2:483418/129‑1 (MQ=255)
                                                                               aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGc                                                      >  1:483418/1‑129 (MQ=255)
                                                                               aGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCtat                                           >  1:847298/1‑140 (MQ=255)
                                                                                 acacCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                         >  2:398203/1‑140 (MQ=255)
                                                                                  cacCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAcc                                        <  1:513072/140‑1 (MQ=255)
                                                                                     ccccATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCaa                                     >  2:634042/1‑140 (MQ=255)
                                                                                        cATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                  <  2:269917/140‑1 (MQ=255)
                                                                                        cATTAAATATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAAt                                  <  1:444159/140‑1 (MQ=255)
                                                                                            aaaTATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                              <  2:1058406/140‑1 (MQ=255)
                                                                                             aaTATGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGt                             <  1:392825/140‑1 (MQ=255)
                                                                                              atatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTc                            >  2:571352/1‑140 (MQ=255)
                                                                                               tatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                         <  2:684512/126‑1 (MQ=255)
                                                                                               tatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATAc                                         >  1:684512/1‑126 (MQ=255)
                                                                                               tatGAAGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCa                           <  1:571352/140‑1 (MQ=255)
                                                                                                   aaGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                       <  1:1203585/140‑1 (MQ=255)
                                                                                                   aaGACGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAt                       >  1:734833/1‑140 (MQ=255)
                                                                                                      aCGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                              >  1:465824/1‑130 (MQ=255)
                                                                                                      aCGAAACTGTTCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTgg                              <  2:465824/130‑1 (MQ=255)
                                                                                                               ttCTGGCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCa           >  1:118513/1‑140 (MQ=255)
                                                                                                                   ggCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTgg       <  2:734833/140‑1 (MQ=255)
                                                                                                                    gCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCt                  >  2:485506/1‑128 (MQ=255)
                                                                                                                    gCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCt                  <  1:485506/128‑1 (MQ=255)
                                                                                                                    gCACACGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTggc      >  2:1139569/1‑140 (MQ=255)
                                                                                                                        acGGCCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGgcggcg  <  2:823369/140‑1 (MQ=255)
                                                                                                                            |                                                                                                                                       
CTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAGCGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCACACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCG  >  NC_000913/2854459‑2854718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A