Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,042,391	Δ1 bp	intergenic (+98/+98)	ygfZ → / ← yqfA	folate‑binding protein/hemolysin‑III family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,042,389	0	C	.	100.0%	204.2 / NA	49	intergenic (+96/+100)	ygfZ/yqfA	folate‑binding protein/hemolysin‑III family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (28/21); total (28/21)

GCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAA  >  NC_000913/3042257‑3042523

gCATATCGAGCCGCTGTCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGAt                                                                                                                                >  2:195760/1‑140 (MQ=255)

 cATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATc                                                                                                                               >  2:1132925/1‑140 (MQ=255)

     tCGAGCCGCTGCCGTATTCGCTCGAAGAGTGAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGAt                                                                                                                           <  2:86525/140‑1 (MQ=255)

                          tCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCg                                                                                                      >  2:226964/1‑140 (MQ=255)

                             aaGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCa                                                                                                   >  2:321690/1‑140 (MQ=255)

                                   aaaTCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTtgtg                                                                                             <  1:195760/140‑1 (MQ=255)

                                        tctTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCg                                                                                        <  2:569456/140‑1 (MQ=255)

                                                           ttATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTgg                                                                     >  1:55756/1‑140 (MQ=255)

                                                            tATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                                                    >  2:1129025/1‑140 (MQ=255)

                                                                gCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGt                                                                <  1:612137/140‑1 (MQ=255)

                                                                gCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGt                                                                <  1:382173/140‑1 (MQ=255)

                                                                 ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                                                    <  1:532207/135‑1 (MQ=255)

                                                                 ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgc                                                                    >  2:532207/1‑135 (MQ=255)

                                                                 ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  1:624204/1‑140 (MQ=255)

                                                                 ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  2:1152202/1‑140 (MQ=255)

                                                                 ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  1:1165775/1‑140 (MQ=255)

                                                                 ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCAAGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                               >  1:969155/1‑140 (MQ=255)

                                                                  cTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt                                                              >  2:739949/1‑140 (MQ=255)

                                                                   tGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCta                                                             >  1:731432/1‑139 (MQ=255)

                                                                          aCGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                      >  1:242894/1‑140 (MQ=255)

                                                                               ggCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 <  1:226964/140‑1 (MQ=255)

                                                                               ggCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 >  2:118140/1‑140 (MQ=255)

                                                                                gcgcGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCtt                                                             >  2:797800/1‑127 (MQ=255)

                                                                                gcgcGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCtt                                                             <  1:797800/127‑1 (MQ=255)

                                                                                gcgcGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa                                                <  2:624204/140‑1 (MQ=255)

                                                                                   cgTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAgg                                             <  1:648324/140‑1 (MQ=255)

                                                                                     tCTTATCATGTCTGGGCTTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 >  2:844020/1‑134 (MQ=255)

                                                                                     tCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                 <  1:844020/134‑1 (MQ=255)

                                                                                               tCTGGGATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTAc                                 >  1:592206/1‑140 (MQ=255)

                                                                                                   ggATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCt                             <  2:592206/140‑1 (MQ=255)

                                                                                                   ggATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCt                             >  1:587210/1‑140 (MQ=255)

                                                                                                   ggATTTGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCt                             <  1:118140/140‑1 (MQ=255)

                                                                                                      tttGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGcc                          <  2:977111/140‑1 (MQ=255)

                                                                                                      tttGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGcc                          <  1:1132925/140‑1 (MQ=255)

                                                                                                       ttGATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGccc                         <  1:1132267/140‑1 (MQ=255)

                                                                                                         gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAt                                    <  1:521894/127‑1 (MQ=255)

                                                                                                         gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAt                                    >  2:521894/1‑127 (MQ=255)

                                                                                                         gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa                       <  2:1165775/140‑1 (MQ=255)

                                                                                                         gATGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCaa                       <  1:519666/140‑1 (MQ=255)

                                                                                                           tGCCTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAata                     <  1:321690/140‑1 (MQ=255)

                                                                                                             ccTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAAtata                   >  2:1032673/1‑140 (MQ=255)

                                                                                                              cTTTATCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATAc                  >  1:1007633/1‑140 (MQ=255)

                                                                                                                  aTCACAACAGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAAt              >  1:391507/1‑140 (MQ=255)

                                                                                                                     acaacaGGGACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGa           <  1:1129025/140‑1 (MQ=255)

                                                                                                                            ggACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAg    >  1:1067737/1‑140 (MQ=255)

                                                                                                                            ggACGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAg    >  2:936685/1‑140 (MQ=255)

                                                                                                                            ggACGAGG‑CCGCAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAg    >  1:34230/1‑140 (MQ=255)

                                                                                                                              aCGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGaa  >  1:623469/1‑140 (MQ=255)

                                                                                                                              aCGAGG‑CCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGaa  >  1:552793/1‑140 (MQ=255)

GCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAA  >  NC_000913/3042257‑3042523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A