Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,276,593	A→G	T438A (ACC→GCC)	garD →	galactarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,276,593	0	A	G	100.0%	172.4 / NA	49	T438A (ACC→GCC)	garD	galactarate dehydratase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/26); total (23/26)

AATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACT  >  NC_000913/3276463‑3276724

aaTTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTg                                                                                                                            <  2:1050934/140‑1 (MQ=255)

aaTTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTg                                                                                                                            >  1:950259/1‑140 (MQ=255)

  ttgttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGt                                                                                                                          >  1:1106914/1‑140 (MQ=255)

   tgttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTa                                                                                                                         <  2:482998/140‑1 (MQ=255)

   tgttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTa                                                                                                                         <  1:594871/140‑1 (MQ=255)

    gttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTAc                                                                                                                        <  2:1187487/140‑1 (MQ=255)

     ttgAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACg                                                                                                                       >  2:854318/1‑140 (MQ=255)

            gCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACg                                                                                                                >  1:1095752/1‑140 (MQ=255)

             cTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACgg                                                                                                               >  2:1133636/1‑140 (MQ=255)

                tCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCt                                                                                                            <  2:272771/140‑1 (MQ=255)

                tCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCt                                                                                                            >  2:185240/1‑140 (MQ=255)

                   cccGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGAt                                                                                                         >  1:314803/1‑140 (MQ=255)

                     cGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATgg                                                                                                       >  2:439331/1‑140 (MQ=255)

                       gTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCg                                                                                                     >  2:1103229/1‑140 (MQ=255)

                          aaCGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTa                                                                                                  >  1:760677/1‑140 (MQ=255)

                           aCGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTAc                                                                                                 <  1:493171/140‑1 (MQ=255)

                            cGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTAcc                                                                                                <  2:80550/140‑1 (MQ=255)

                                           ggATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGc                                                                                 <  2:314803/140‑1 (MQ=255)

                                           ggATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGc                                                                                 <  1:151680/140‑1 (MQ=255)

                                                  tttACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGc                                                                          >  1:780962/1‑140 (MQ=255)

                                                  tttACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGc                                                                          <  2:1095752/140‑1 (MQ=255)

                                                   ttACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCa                                                                         <  2:950259/140‑1 (MQ=255)

                                                         cgcgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGc                                                                   >  2:939712/1‑140 (MQ=255)

                                                         cgcgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGc                                                                   >  2:276537/1‑140 (MQ=255)

                                                         cgcgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGc                                                                   >  2:245317/1‑140 (MQ=255)

                                                           cgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTg                                                                 >  2:547282/1‑140 (MQ=255)

                                                           cgACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTg                                                                 >  2:272560/1‑140 (MQ=255)

                                                               gccagccaGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGa                                                             >  1:824439/1‑140 (MQ=255)

                                                                   gccagcGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCg                                                         <  2:824439/140‑1 (MQ=255)

                                                                    ccagcGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGc                                                        <  2:1106914/140‑1 (MQ=255)

                                                                        cGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGt                                                    >  1:1201056/1‑140 (MQ=255)

                                                                            tttGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGa                                                <  1:854318/140‑1 (MQ=255)

                                                                                tCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTa                                            <  2:760677/140‑1 (MQ=255)

                                                                                tCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTa                                            <  2:457178/140‑1 (MQ=255)

                                                                                  tgtgGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAAt                                          <  1:1103229/140‑1 (MQ=255)

                                                                                  tgtgGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAAt                                          <  1:185240/140‑1 (MQ=255)

                                                                                                 gTGGCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCggg                           <  1:547282/140‑1 (MQ=255)

                                                                                                   ggCTTCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCa                         <  2:1201056/140‑1 (MQ=255)

                                                                                                      ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGTCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       >  2:1136317/1‑139 (MQ=255)

                                                                                                      ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       <  1:224240/139‑1 (MQ=255)

                                                                                                      ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       >  2:224240/1‑139 (MQ=255)

                                                                                                      ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAcc                       <  1:1136317/139‑1 (MQ=255)

                                                                                                      ttCGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCa                      >  2:1211517/1‑140 (MQ=255)

                                                                                                        cGGGTATCACAGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATc                    >  2:1156864/1‑140 (MQ=255)

                                                                                                                 caGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCggg                           >  1:1185807/1‑124 (MQ=255)

                                                                                                                 caGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCggg                           <  2:1185807/124‑1 (MQ=255)

                                                                                                                 caGTGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGc           <  1:1156864/140‑1 (MQ=255)

                                                                                                                    tGCAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGCACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCgaa        >  2:731663/1‑140 (MQ=255)

                                                                                                                      cAAGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCgaaga      <  1:245317/140‑1 (MQ=255)

                                                                                                                        aGTGTTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGaaa    <  1:731663/140‑1 (MQ=255)

                                                                                                                          tgtTTACGGCCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACt  <  2:416742/140‑1 (MQ=255)

AATTGTTGAAGTGCTGTCGCCCGGTCAACGCCCGACTAAACGCGGATTAATTTACGCCGCGACGCCAGCCAGCGATTTTGTCTGTGGCACGCAACAGGTGGCTTCGGGTATCACAGTGCAAGTGTTTACGACCGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACT  >  NC_000913/3276463‑3276724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A