Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F28 I1 R1
|
228 |
65.7 |
2434974 |
91.7% |
2232871 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,495,452 |
T→C |
V481A (GTT→GCT) |
nirB → |
nitrite reductase catalytic subunit NirB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,495,452 | 0 | T | C | 96.4%
| 186.0
/ ‑2.3
| 56 | V481A (GTT→GCT) | nirB | nitrite reductase catalytic subunit NirB |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (27/27); total (29/27) |
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
TGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACC > NC_000913/3495321‑3495587
|
tgcCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAATCAGGGCATCGAAGCTaacaaca > 1:776139/1‑140 (MQ=255)
tgcCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTaacaaca > 1:553665/1‑140 (MQ=255)
ccATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTaacaacaac > 2:424356/1‑140 (MQ=255)
caaAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCg < 1:234397/140‑1 (MQ=255)
caaAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCg < 1:275315/140‑1 (MQ=255)
aaGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGaa < 2:553665/140‑1 (MQ=255)
ggCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAaca < 1:855340/140‑1 (MQ=255)
ggCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAaca < 1:417182/140‑1 (MQ=255)
tGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACtt > 1:1013012/1‑140 (MQ=255)
gCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAac > 1:700214/1‑135 (MQ=255)
gCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAac < 2:700214/135‑1 (MQ=255)
acacaGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCt > 2:113602/1‑140 (MQ=255)
acaGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTa < 1:446086/140‑1 (MQ=255)
aGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTAtt > 1:791874/1‑140 (MQ=255)
gTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTc < 2:88398/140‑1 (MQ=255)
gcggcgCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTcgcg > 1:21978/1‑140 (MQ=255)
ggcgCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAATCAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTc < 2:776139/140‑1 (MQ=255)
ggcgCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTc < 2:448519/140‑1 (MQ=255)
ctgaaagctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGa < 2:472514/140‑1 (MQ=255)
tgaaagctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGaa < 1:884715/140‑1 (MQ=255)
agctgaaaCCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGt < 1:113602/140‑1 (MQ=255)
aaaGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGAt < 2:708416/140‑1 (MQ=255)
aGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATcc > 1:339392/1‑140 (MQ=255)
aGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGaa > 2:1196812/1‑124 (MQ=255)
aGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGaa < 1:1196812/124‑1 (MQ=255)
tACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTg > 1:973332/1‑140 (MQ=255)
aCTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGa > 1:1117953/1‑140 (MQ=255)
tGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAg < 1:684924/140‑1 (MQ=255)
tGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAg < 2:973332/140‑1 (MQ=255)
gCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGc > 1:698872/1‑140 (MQ=255)
tGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCAt > 1:198757/1‑140 (MQ=255)
cGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCT‑ACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTaa > 1:340079/1‑140 (MQ=255)
tGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCAtt > 1:591036/1‑136 (MQ=255)
tGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCAtt < 2:591036/136‑1 (MQ=255)
gCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAAc > 1:1190129/1‑140 (MQ=255)
gCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAAc < 1:455838/140‑1 (MQ=255)
gTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAActgct > 2:936634/1‑140 (MQ=255)
ggTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAac < 2:791874/140‑1 (MQ=255)
gAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCa < 1:676015/140‑1 (MQ=255)
gAACGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCa < 2:198757/140‑1 (MQ=255)
aaCGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCaa > 1:301351/1‑140 (MQ=255)
aaCGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCaa > 1:980230/1‑140 (MQ=255)
aaCGCGGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCaa > 2:194628/1‑140 (MQ=255)
gcgGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAgg > 1:471225/1‑140 (MQ=255)
gcgGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAgg < 1:1181315/140‑1 (MQ=255)
cgGAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGc > 2:880936/1‑140 (MQ=255)
ggAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCt > 2:182972/1‑140 (MQ=255)
ggAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCa > 2:197437/1‑134 (MQ=255)
ggAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCa < 1:197437/134‑1 (MQ=255)
ggAACTGGCGAAACAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCt > 2:879183/1‑140 (MQ=255)
aaaCAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGa < 2:698872/140‑1 (MQ=255)
aCAGGGCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAg > 2:139958/1‑140 (MQ=255)
cAGGGCATCGAAGCT‑ACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGtt < 2:340079/140‑1 (MQ=255)
gCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGt > 1:194965/1‑140 (MQ=255)
gCATCGAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCGACGGTTGTGAAGTTTGt > 2:1083063/1‑140 (MQ=255)
gAAGCTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAaacc < 1:936634/140‑1 (MQ=255)
|
TGCCATCAACAAAGGCTGCCACACAGTTGCGGCGCTGAAAGCTGAAACCAAAGCGGGTACTGGCTGCGGTGGCTGTATCCCGCTGGTCACTCAGGTACTGAACGCGGAACTGGCGAAACAGGGCATCGAAGTTAACAACAACCTGTGCGAACACTTTGCTTATTCGCGTCAGGAACTGTTCCATTTGATCCGCGTTGAAGGCATTAAAACCTTCGAAGAACTGCTGGCGAAACACGGCAAAGGCTACGGTTGTGAAGTTTGTAAACC > NC_000913/3495321‑3495587
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
