Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,705,838	T→C	G24G (GGT→GGC)	rsxA →	SoxR [2Fe‑2S] reducing system protein RsxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,705,838	0	T	C	100.0%	148.8 / NA	43	G24G (GGT→GGC)	rsxA	SoxR [2Fe‑2S] reducing system protein RsxA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/21); total (22/21)

AACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGATTTTGATCCCACTTAATCTG  >  NC_000913/1705704‑1705973

aaCCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTg                                                                                                                                    >  1:256513/1‑140 (MQ=255)

   cTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTcc                                                                                                                                 <  2:223624/140‑1 (MQ=255)

        gtCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTa                                                                                                                            >  2:751158/1‑140 (MQ=255)

                    ggATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCa                                                                                                                <  2:707603/140‑1 (MQ=255)

                               taataGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGGACTGTACTGGTCAATAACTTTGTACTGATCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGaa                                                                                                     >  2:1125510/1‑140 (MQ=255)

                               taataGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGaa                                                                                                     >  1:763729/1‑140 (MQ=255)

                               taataGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGaa                                                                                                     >  1:145995/1‑140 (MQ=255)

                                     gcggcTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTCACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACcgcg                                                                                               <  2:1175612/140‑1 (MQ=255)

                                      cggcTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGa                                                                                              <  2:145995/140‑1 (MQ=255)

                                       ggcTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACcgcg                                                                                               <  1:326637/138‑1 (MQ=255)

                                       ggcTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACcgcg                                                                                               >  2:326637/1‑138 (MQ=255)

                                        gcTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATg                                                                                            <  1:602955/140‑1 (MQ=255)

                                                  ttCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGc                                                                                  <  1:1046549/140‑1 (MQ=255)

                                                   tCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCt                                                                                 >  2:133946/1‑140 (MQ=255)

                                                       gCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGa                                                                                              >  2:243659/1‑123 (MQ=255)

                                                       gCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGa                                                                                              <  1:243659/123‑1 (MQ=255)

                                                       gCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATg                                                                                      <  1:606225/131‑1 (MQ=255)

                                                       gCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATg                                                                                      >  2:606225/1‑131 (MQ=255)

                                                       gCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGca                                                                             >  2:717066/1‑140 (MQ=255)

                                                              aaTGACTGACTACCTGTTACTGTTTGTCGGAACTGTANNGNTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGt                                                                      <  1:956465/140‑1 (MQ=255)

                                                                    tgactACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGtga                                                                <  1:133946/140‑1 (MQ=255)

                                                                            tGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTg                                                        <  1:717066/140‑1 (MQ=255)

                                                                                aCTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGt                                                    <  1:1125510/140‑1 (MQ=255)

                                                                                  tGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCt                                                  >  1:683306/1‑140 (MQ=255)

                                                                                   gTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTa                                                 >  2:239825/1‑140 (MQ=255)

                                                                                        tCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTgc                                            >  1:915754/1‑140 (MQ=255)

                                                                                          ggAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTgcgc                                          <  2:763729/140‑1 (MQ=255)

                                                                                          ggAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTgcgc                                          <  1:677836/140‑1 (MQ=255)

                                                                                                  aCTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTa                                  <  2:915754/140‑1 (MQ=255)

                                                                                                      gTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGa                              >  1:882430/1‑140 (MQ=255)

                                                                                                      gTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGa                              >  1:692335/1‑140 (MQ=255)

                                                                                                      gTCAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGa                              >  1:63072/1‑140 (MQ=255)

                                                                                                        cAATAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATa                            >  1:650413/1‑140 (MQ=255)

                                                                                                           tAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTAt                                 <  1:136151/132‑1 (MQ=255)

                                                                                                           tAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTAt                                 >  2:136151/1‑132 (MQ=255)

                                                                                                           tAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGt                         <  2:256513/140‑1 (MQ=255)

                                                                                                           tAACTTTGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGt                         <  2:692335/140‑1 (MQ=255)

                                                                                                                 tGTACTGGTCAAGTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGAttt                   >  2:668954/1‑140 (MQ=255)

                                                                                                                             gTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGATTTTGATCCCACTTa       >  2:551237/1‑140 (MQ=255)

                                                                                                                             gTTTCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGATTTTGATCCCACTTa       >  2:1032369/1‑140 (MQ=255)

                                                                                                                              tttCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGATTTTGATCCCACTTaa      <  1:551237/140‑1 (MQ=255)

                                                                                                                              tttCTCGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGATTTTGATCCCACTTaa      <  1:1032369/140‑1 (MQ=255)

                                                                                                                                  tcGGCCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGATTTTGATCCCACTTAATCTg  >  2:1153272/1‑140 (MQ=255)

AACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAACAACGTTTGTGATGACGCTGGCGTCTATTTGCGCCTGGCTTATCGATACGTGGATTTTGATCCCACTTAATCTG  >  NC_000913/1705704‑1705973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A