Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,950,998	T→C	V56A (GTT→GCT)	yecD →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,950,998	0	T	C	100.0%	187.6 / NA	53	V56A (GTT→GCT)	yecD	putative hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/26); total (27/26)

GATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAA  >  NC_000913/1950870‑1951134

gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTg                                                                                                                               >  2:349249/1‑140 (MQ=255)

    gATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGa                                                                                                                           <  1:785410/140‑1 (MQ=255)

    gATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGa                                                                                                                           <  2:1227065/140‑1 (MQ=255)

     aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGAt                                                                                                                          <  1:603394/140‑1 (MQ=255)

     aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGAt                                                                                                                          <  2:188458/140‑1 (MQ=255)

            agaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTg                                                                                                                   <  2:326869/140‑1 (MQ=255)

             gaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGTCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGa                                                                                                                  <  1:924277/140‑1 (MQ=255)

             gaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTTTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGa                                                                                                                  >  1:1010190/1‑140 (MQ=255)

             gaagGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGa                                                                                                                  >  1:226319/1‑140 (MQ=255)

                ggCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGc                                                                                                               >  2:30372/1‑140 (MQ=255)

                          cTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTTTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAg                                                                                                     <  2:1010190/140‑1 (MQ=255)

                          cTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAg                                                                                                     <  2:226319/140‑1 (MQ=255)

                               gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                                                                                                >  2:530377/1‑140 (MQ=255)

                               gCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                                                                                                >  1:867555/1‑140 (MQ=255)

                                    tggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                                                                                           >  1:1213946/1‑140 (MQ=255)

                                        ccACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCC‑‑GTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCt                                                                                     >  2:525123/1‑140 (MQ=255)

                                             tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTATGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAc                                                                                  <  1:972527/140‑1 (MQ=255)

                                             tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAc                                                                                  <  1:832907/140‑1 (MQ=255)

                                                tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  2:796537/1‑136 (MQ=255)

                                                tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  2:21201/1‑136 (MQ=255)

                                                tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  1:21201/136‑1 (MQ=255)

                                                tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  2:608673/1‑136 (MQ=255)

                                                tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  1:608673/136‑1 (MQ=255)

                                                tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  1:796537/136‑1 (MQ=255)

                                                tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCgg                                                                               >  2:1130494/1‑140 (MQ=255)

                                                tGCCGATGAGGGAGTTAATCGCGCCGGGGAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCgg                                                                               >  2:1089174/1‑140 (MQ=255)

                                                 gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   >  1:33123/1‑135 (MQ=255)

                                                 gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa                                                                                   <  2:33123/135‑1 (MQ=255)

                                                 gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                                              >  1:848683/1‑140 (MQ=255)

                                                 gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGc                                                                              >  2:599026/1‑140 (MQ=255)

                                                  ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCt                                                                             >  2:957161/1‑140 (MQ=255)

                                                   cGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTa                                                                            >  1:26706/1‑140 (MQ=255)

                                                   cGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTa                                                                            <  1:30372/140‑1 (MQ=255)

                                                           tAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCC‑‑GTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGcc                                                                  <  1:525123/140‑1 (MQ=255)

                                                            aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGc                                                                   >  1:1142458/1‑140 (MQ=255)

                                                                   cgcgCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGaaaa                                                            >  1:266503/1‑140 (MQ=255)

                                                                    gcgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAAt                                                           >  2:302520/1‑140 (MQ=255)

                                                                     cgcCGGGAAGCTGGCGGTGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAAtt                                                          >  1:241525/1‑140 (MQ=255)

                                                                       ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        >  2:168739/1‑140 (MQ=255)

                                                                       ccGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        >  1:463785/1‑140 (MQ=255)

                                                                               cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACa                                                <  1:349249/140‑1 (MQ=255)

                                                                                tGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACAt                                               <  1:1130494/140‑1 (MQ=255)

                                                                                 ggcggcGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATc                                              <  2:26706/140‑1 (MQ=255)

                                                                                    ggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctg                                           <  1:599026/140‑1 (MQ=255)

                                                                                    ggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctg                                           <  2:867555/140‑1 (MQ=255)

                                                                                      cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCctgct                                         <  1:302520/140‑1 (MQ=255)

                                                                                        aaaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        <  1:566982/123‑1 (MQ=255)

                                                                                        aaaTTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATtgg                                                        >  2:566982/1‑123 (MQ=255)

                                                                                            ttCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTa                                   >  1:1259593/1‑140 (MQ=255)

                                                                                             tCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAg                                  <  2:1142458/140‑1 (MQ=255)

                                                                                                  ccAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCa                             <  2:848683/140‑1 (MQ=255)

                                                                                                          cAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAg                     <  1:530377/140‑1 (MQ=255)

                                                                                                                gtgtTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGatat               >  1:1057952/1‑140 (MQ=255)

                                                                                                                           gcgcgcTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAAtcatca    <  2:463785/140‑1 (MQ=255)

                                                                                                                           gcgcgcTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAAtcatca    >  2:1111544/1‑140 (MQ=255)

                                                                                                                            cgcgCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCaa   >  2:287722/1‑140 (MQ=255)

                                                                                                                             gcgcTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCaaa  <  2:1213946/140‑1 (MQ=255)

GATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGTGTTGCCCGAAAATTGGTGGCAACATCCTGCTGCATTAGGTGCAACCGACAGCGATATCGAAATCATCAAA  >  NC_000913/1950870‑1951134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A