Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,495	G→A	A210T (GCC→ACC)	fliH →	flagellar biosynthesis protein FliH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,495	0	G	A	100.0%	260.0 / NA	74	A210T (GCC→ACC)	fliH	flagellar biosynthesis protein FliH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (33/41); total (33/41)

GCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTAC  >  NC_000913/2016358‑2016629

gcgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACAcc                                                                                                                                      >  2:493979/1‑140 (MQ=255)

gcgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTAAAGTTTGCATGGCTTGCGCTGGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACAcc                                                                                                                                      <  2:778636/140‑1 (MQ=255)

 cgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCa                                                                                                                                     >  2:682605/1‑140 (MQ=255)

            atgatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACt                                                                                                                          >  2:335778/1‑140 (MQ=255)

              gatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCg                                                                                                                        <  1:312510/140‑1 (MQ=255)

              gatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCg                                                                                                                        <  2:566376/140‑1 (MQ=255)

                   gCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGc                                                                                                                   <  1:719868/140‑1 (MQ=255)

                   gCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGc                                                                                                                   <  2:422533/140‑1 (MQ=255)

                   gCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGc                                                                                                                   <  1:133187/140‑1 (MQ=255)

                            ggATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAActct                                                                                                          >  1:760812/1‑140 (MQ=255)

                             gatgatATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTg                                                                                                         <  2:184620/140‑1 (MQ=255)

                                gatATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCg                                                                                                      >  1:520140/1‑140 (MQ=255)

                                   atGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCt                                                                                                   >  1:280170/1‑140 (MQ=255)

                                    tGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTg                                                                                                  <  2:843834/140‑1 (MQ=255)

                                     gCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTgg                                                                                                 >  1:1075690/1‑140 (MQ=255)

                                      cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:682605/140‑1 (MQ=255)

                                      cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:132814/140‑1 (MQ=255)

                                      cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  2:810931/140‑1 (MQ=255)

                                      cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:142266/140‑1 (MQ=255)

                                      cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                                                                <  1:340922/140‑1 (MQ=255)

                                            gcTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCAcc                                                                                          >  1:59510/1‑140 (MQ=255)

                                              tACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAg                                                                                        <  2:59510/140‑1 (MQ=255)

                                              tACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAg                                                                                        <  1:335778/140‑1 (MQ=255)

                                                ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGa                                                                                      <  2:858419/140‑1 (MQ=255)

                                                ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGa                                                                                      >  2:284005/1‑140 (MQ=255)

                                                ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGa                                                                                      >  1:110185/1‑140 (MQ=255)

                                                 cTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAg                                                                                     <  2:520140/140‑1 (MQ=255)

                                                  ttAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAgt                                                                                    <  2:411466/140‑1 (MQ=255)

                                                   tAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAgtg                                                                                   <  2:760812/140‑1 (MQ=255)

                                                          gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTa                                                                              >  2:111655/1‑138 (MQ=255)

                                                          gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTa                                                                              <  1:111655/138‑1 (MQ=255)

                                                          gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                                                                            >  2:340307/1‑140 (MQ=255)

                                                          gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                                                                            >  1:846762/1‑140 (MQ=255)

                                                          gCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                                                                            >  1:256754/1‑140 (MQ=255)

                                                           cATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCAcc                                                                                          >  1:205646/1‑125 (MQ=255)

                                                           cATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCAcc                                                                                          <  2:205646/125‑1 (MQ=255)

                                                               gctggcGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                                                                       <  2:645148/140‑1 (MQ=255)

                                                               gctggcGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                                                                       <  2:846762/140‑1 (MQ=255)

                                                                        tGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGa                                                              >  1:566644/1‑140 (MQ=255)

                                                                          cGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACt                                                            >  1:700287/1‑140 (MQ=255)

                                                                           ggggCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTc                                                           <  1:493979/140‑1 (MQ=255)

                                                                             ggCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGc                                                         <  1:147633/140‑1 (MQ=255)

                                                                                  tCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCt                                                    <  2:256754/140‑1 (MQ=255)

                                                                                       cccTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCa                                               <  1:1106502/140‑1 (MQ=255)

                                                                                         cTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  1:533217/1‑140 (MQ=255)

                                                                                         cTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:284005/140‑1 (MQ=255)

                                                                                            cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:1136993/137‑1 (MQ=255)

                                                                                            cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  2:1136993/1‑137 (MQ=255)

                                                                                            cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  2:1052800/1‑140 (MQ=255)

                                                                                            cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  1:1016523/1‑140 (MQ=255)

                                                                                            cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  2:668977/1‑140 (MQ=255)

                                                                                            cATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTg                                          >  1:123046/1‑140 (MQ=255)

                                                                                             aTCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:856325/136‑1 (MQ=255)

                                                                                             aTCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  2:856325/1‑136 (MQ=255)

                                                                                              tCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  2:734628/135‑1 (MQ=255)

                                                                                              tCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  1:734628/1‑135 (MQ=255)

                                                                                                  ggcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  2:1182072/131‑1 (MQ=255)

                                                                                                  ggcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  1:1182072/1‑131 (MQ=255)

                                                                                                   gcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACNAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             >  2:686937/1‑130 (MQ=255)

                                                                                                   gcggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACg                                             <  1:686937/130‑1 (MQ=255)

                                                                                                     ggcTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACttt                                 <  2:123046/140‑1 (MQ=255)

                                                                                                        tGTAAAGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGaa                              >  2:459961/1‑140 (MQ=255)

                                                                                                             aGTCTCCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCaa                         <  1:1052800/140‑1 (MQ=255)

                                                                                                                 tcCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATg                     >  1:171021/1‑140 (MQ=255)

                                                                                                                 tcCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATg                     <  2:700287/140‑1 (MQ=255)

                                                                                                                 tcCGCCGATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATg                     >  1:454087/1‑140 (MQ=255)

                                                                                                                   cgccgATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGgc                   >  2:590795/1‑140 (MQ=255)

                                                                                                                    gccgATGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGgcg                  >  2:508071/1‑140 (MQ=255)

                                                                                                                        aTGAAGGCGATCTCGACACCAGTGTCGCCACTCGTTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGt              >  2:1230056/1‑140 (MQ=255)

                                                                                                                         tGAAGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGtt             <  2:171021/140‑1 (MQ=255)

                                                                                                                           aaGGCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGc           <  1:508071/140‑1 (MQ=255)

                                                                                                                              gCGATCTCGACACCAGTGTCGCCACTCGTTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTg        <  1:1230056/140‑1 (MQ=255)

                                                                                                                              gCGATCTCGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTg        <  1:590795/140‑1 (MQ=255)

                                                                                                                                    tcGACACCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTAc  <  1:1140067/140‑1 (MQ=255)

GCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAACCACGCTGGATAACTTTGAAGCCAAAATGGCGCAGTTGCCTGCGGTAC  >  NC_000913/2016358‑2016629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A