Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,306,567	T→C	E63G (GAG→GGG)	mqo ←	malate:quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,306,567	0	T	C	100.0%	177.0 / NA	51	E63G (GAG→GGG)	mqo	malate:quinone oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/20); total (31/20)

GGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGA  >  NC_000913/2306434‑2306700

ggCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCccacca                                                                                                                                 <  2:603515/140‑1 (MQ=255)

     cTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTc                                                                                                                            <  2:504690/140‑1 (MQ=255)

                aTACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTc                                                                                                                 <  2:1122966/140‑1 (MQ=255)

                      aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAGCCCGTTCGAACTCTCCTGCGCGGCACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCt                                                                                                            >  2:666934/1‑140 (MQ=255)

                      aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:300794/1‑140 (MQ=255)

                      aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:1131654/1‑140 (MQ=255)

                      aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:479663/1‑140 (MQ=255)

                      aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  1:734098/1‑140 (MQ=255)

                      aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:1180589/1‑140 (MQ=255)

                      aTGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTc                                                                                                           >  2:1142528/1‑140 (MQ=255)

                       tgctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTcc                                                                                                          >  1:841098/1‑140 (MQ=255)

                         ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  2:1179772/140‑1 (MQ=255)

                         ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  1:637540/140‑1 (MQ=255)

                         ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  1:1180589/140‑1 (MQ=255)

                         ctgcCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAg                                                                                                        <  1:1131654/140‑1 (MQ=255)

                            ccATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTc                                                                                                     <  1:576455/140‑1 (MQ=255)

                              aTCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTcgc                                                                                                   >  1:41124/1‑140 (MQ=255)

                                 ggCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTa                                                                                                <  1:209789/140‑1 (MQ=255)

                                 ggCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCACACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTa                                                                                                >  2:1217525/1‑140 (MQ=255)

                                               tgtAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAAc                                                                                  >  2:1023182/1‑140 (MQ=255)

                                                 tAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATCCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGt                                                                                >  1:525742/1‑140 (MQ=255)

                                                    ttcagttcCATCAGTGCAGAATGCCCGGTTCCGGCGTTATCCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGgc                                                                             <  2:525742/140‑1 (MQ=255)

                                                         ttcCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGC‑ACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCAt                                                                       >  1:214344/1‑140 (MQ=255)

                                                         ttcCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTACGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCa                                                                        >  1:1148252/1‑140 (MQ=255)

                                                              tCAGTGTAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATg                                                                   >  2:1254125/1‑140 (MQ=255)

                                                                  tGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATgccgc                                                               <  2:41124/140‑1 (MQ=255)

                                                                    cAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATgccgccg                                                             >  2:538660/1‑140 (MQ=255)

                                                                      gAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGC‑ACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCa                                                          <  2:214344/140‑1 (MQ=255)

                                                                       aaTGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCa                                                          >  1:1020110/1‑140 (MQ=255)

                                                                         tGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAAt                                                        >  2:441847/1‑140 (MQ=255)

                                                                         tGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAAt                                                        <  1:479663/140‑1 (MQ=255)

                                                                         tGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAAt                                                        <  1:300794/140‑1 (MQ=255)

                                                                            ccGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCaa                                                     <  1:441847/140‑1 (MQ=255)

                                                                            ccGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCACACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCaa                                                     <  1:1217525/140‑1 (MQ=255)

                                                                               gTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAg                                                  <  2:1020110/140‑1 (MQ=255)

                                                                                 tCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTa                                                >  2:987014/1‑140 (MQ=255)

                                                                                 tCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTa                                                >  1:1056565/1‑140 (MQ=255)

                                                                                    ggCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACAt                                             <  1:538660/140‑1 (MQ=255)

                                                                                                g‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGct                                 >  2:655086/1‑140 (MQ=255)

                                                                                                   cGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGctcc                               >  1:1252334/1‑140 (MQ=255)

                                                                                                      tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  2:341497/1‑140 (MQ=255)

                                                                                                      tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  1:570934/1‑140 (MQ=255)

                                                                                                      tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  2:536906/1‑140 (MQ=255)

                                                                                                      tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            <  2:841098/140‑1 (MQ=255)

                                                                                                      tCGAACTCTCCTGCGCGACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTACAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCg                            >  1:121092/1‑140 (MQ=255)

                                                                                                                   cgcgACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGAcgctttcgctt               >  1:256419/1‑140 (MQ=255)

                                                                                                                   cgcgACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCC‑CGGAcgctttcgcttt              >  2:823390/1‑140 (MQ=255)

                                                                                                                      gACACCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTg            >  1:858228/1‑140 (MQ=255)

                                                                                                                        cacCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGa                          <  1:812793/124‑1 (MQ=255)

                                                                                                                        cacCCTCCAGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGa                          >  2:812793/1‑124 (MQ=255)

                                                                                                                                aGGCGCCCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGa  >  2:62184/1‑140 (MQ=255)

GGCGACTGCTTTTTCAATACTGATGCTGCCATCGGCGTTTTGCGGGGTGTAGTTCAGTTCCATCAGTGCAGAATGCCCGGTTCCGGCGTTATTCCAG‑CCGTTCGAACTCTCCTGCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGA  >  NC_000913/2306434‑2306700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A