Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,161	T→C	D87G (GAC→GGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,161	0	T	C	100.0%	198.4 / NA	56	D87G (GAC→GGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/26); total (30/26)

ATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCT  >  NC_000913/2339025‑2339296

aTGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCAt                                                                                                                                      >  1:1255529/1‑140 (MQ=255)

   ggCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGa                                                                                                                                   >  2:657363/1‑140 (MQ=255)

     cAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAcc                                                                                                                                 >  1:920636/1‑140 (MQ=255)

       aTTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgc                                                                                                                               >  1:1217125/1‑140 (MQ=255)

         tttCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGAccgccg                                                                                                                             <  1:276827/140‑1 (MQ=255)

           tCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAg                                                                                                                           <  2:1065422/140‑1 (MQ=255)

           tCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAg                                                                                                                           <  1:262759/140‑1 (MQ=255)

            cGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCTGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGt                                                                                                                          <  2:920636/140‑1 (MQ=255)

            cGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGt                                                                                                                          <  2:705246/140‑1 (MQ=255)

                      aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATgggg                                                                                                                <  1:153705/140‑1 (MQ=255)

                        ttCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGAt                                                                                                              <  2:1217125/140‑1 (MQ=255)

                                  cGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTAcc                                                                                                    >  1:465675/1‑140 (MQ=255)

                                    cATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGa                                                                                                  >  1:715138/1‑140 (MQ=255)

                                    cATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGa                                                                                                  >  1:62554/1‑140 (MQ=255)

                                     aTTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGAt                                                                                                 >  2:255643/1‑140 (MQ=255)

                                       tGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTa                                                                                               >  1:316573/1‑140 (MQ=255)

                                         ccgccgCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACg                                                                                             <  2:62554/140‑1 (MQ=255)

                                                       ccGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACg                                                                               >  2:1137764/1‑140 (MQ=255)

                                                           cGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCa                                                                           <  2:337798/140‑1 (MQ=255)

                                                            gATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGGCCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAg                                                                          >  2:630787/1‑140 (MQ=255)

                                                              tAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGAt                                                                        >  1:1219548/1‑140 (MQ=255)

                                                                      aaGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtata                                                                >  1:889105/1‑140 (MQ=255)

                                                                          tACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCt                                                            <  2:1255529/140‑1 (MQ=255)

                                                                          tACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCt                                                            <  2:1219548/140‑1 (MQ=255)

                                                                           aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                                 <  1:32479/134‑1 (MQ=255)

                                                                           aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtat                                                                 >  2:32479/1‑134 (MQ=255)

                                                                           aCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCtt                                                           >  1:741973/1‑140 (MQ=255)

                                                                                gACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGGCCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTc                                                      <  1:630787/140‑1 (MQ=255)

                                                                                     tCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtata                                                                <  2:1051833/125‑1 (MQ=255)

                                                                                     tCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTtata                                                                >  1:1051833/1‑125 (MQ=255)

                                                                                      cTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                <  1:255643/140‑1 (MQ=255)

                                                                                      cTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCa                                                <  1:150176/140‑1 (MQ=255)

                                                                                             cATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTa                                         >  2:935664/1‑140 (MQ=255)

                                                                                             cATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTa                                         >  1:146789/1‑140 (MQ=255)

                                                                                               tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                       <  2:741973/140‑1 (MQ=255)

                                                                                               tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                       >  1:72161/1‑140 (MQ=255)

                                                                                               tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                       >  2:413191/1‑140 (MQ=255)

                                                                                               tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                       >  1:513273/1‑140 (MQ=255)

                                                                                               tataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGt                                       >  2:296711/1‑140 (MQ=255)

                                                                                                ataACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTa                                      >  1:1025069/1‑140 (MQ=255)

                                                                                                    cGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGtt                                  >  1:825251/1‑140 (MQ=255)

                                                                                                     gCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTc                                 <  1:935664/140‑1 (MQ=255)

                                                                                                                    gcgcCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcg                  <  1:282038/140‑1 (MQ=255)

                                                                                                                    gcgcCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcg                  <  2:889105/140‑1 (MQ=255)

                                                                                                                     cgcCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcgc                 >  1:240251/1‑140 (MQ=255)

                                                                                                                     cgcCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcg                  <  1:779760/139‑1 (MQ=255)

                                                                                                                     cgcCATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTAcg                  >  2:779760/1‑139 (MQ=255)

                                                                                                                       ccATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa               <  1:657363/140‑1 (MQ=255)

                                                                                                                       ccATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa               <  2:146789/140‑1 (MQ=255)

                                                                                                                       ccATGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGa               <  1:296711/140‑1 (MQ=255)

                                                                                                                         aTGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACg             <  2:715138/140‑1 (MQ=255)

                                                                                                                          tGCGGACGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACgg            >  2:689758/1‑140 (MQ=255)

                                                                                                                                cGATCGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTAcc      <  1:1137764/140‑1 (MQ=255)

                                                                                                                                    cGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCt  >  1:462842/1‑140 (MQ=255)

                                                                                                                                    cGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCt  >  2:60930/1‑140 (MQ=255)

                                                                                                                                    cGTGCCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCt  >  1:717082/1‑140 (MQ=255)

ATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCT  >  NC_000913/2339025‑2339296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A