Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,770,794	G→A	T38T (ACG→ACA)	yfjS →	CP4‑57 prophage; inner membrane lipoprotein YfjS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,770,794	0	G	A	100.0%	194.4 / NA	59	T38T (ACG→ACA)	yfjS	CP4‑57 prophage; inner membrane lipoprotein YfjS
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/29); total (30/29)

CTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACGCCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAGT  >  NC_000913/2770661‑2770929

cTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCcg                                                                                                                                   <  1:1171975/140‑1 (MQ=255)

cTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCcg                                                                                                                                   <  2:535992/140‑1 (MQ=255)

cTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCcg                                                                                                                                   <  2:1088437/140‑1 (MQ=255)

          ggAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCg                                                                                                                         >  2:698501/1‑140 (MQ=255)

          ggAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCg                                                                                                                         >  1:294673/1‑140 (MQ=255)

                    atGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAg                                                                                                               <  1:486014/140‑1 (MQ=255)

                         aCGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTc                                                                                                          <  2:796437/140‑1 (MQ=255)

                              aaaCACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTTAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGgat                                                                                                     <  2:1167473/140‑1 (MQ=255)

                                 cacTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGgatgat                                                                                                  <  2:668824/140‑1 (MQ=255)

                                    tACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTc                                                                                               >  1:893825/1‑140 (MQ=255)

                                    tACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTc                                                                                               >  2:223457/1‑140 (MQ=255)

                                     aCCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCa                                                                                              >  2:630097/1‑140 (MQ=255)

                                      ccTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAg                                                                                             >  1:536556/1‑140 (MQ=255)

                                           gctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaag                                                                                        <  2:294673/140‑1 (MQ=255)

                                           gctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaag                                                                                        >  2:859679/1‑140 (MQ=255)

                                            ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       >  1:592157/1‑140 (MQ=255)

                                            ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       >  1:608437/1‑140 (MQ=255)

                                            ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       <  1:602348/140‑1 (MQ=255)

                                            ctgGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCgaaga                                                                                       >  2:1096459/1‑140 (MQ=255)

                                                 gcTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGt                                                                                  >  1:120110/1‑140 (MQ=255)

                                                    ggTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTcac                                                                               >  2:300412/1‑140 (MQ=255)

                                                               ctctGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTtc                                                                    >  1:785499/1‑140 (MQ=255)

                                                               ctctGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTtc                                                                    >  2:317221/1‑140 (MQ=255)

                                                                    tttCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATc                                                               <  2:782080/140‑1 (MQ=255)

                                                                      tCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCg                                                             >  2:160257/1‑140 (MQ=255)

                                                                          aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTg                                                                         >  1:680130/1‑124 (MQ=255)

                                                                          aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTg                                                                         <  2:680130/124‑1 (MQ=255)

                                                                          aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGtct                                                         >  1:338107/1‑140 (MQ=255)

                                                                          aGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGtct                                                         <  1:249766/140‑1 (MQ=255)

                                                                            cgcCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCa                                                       >  1:636454/1‑140 (MQ=255)

                                                                            cgcCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCa                                                       >  1:631174/1‑140 (MQ=255)

                                                                              ccTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCAcc                                                     >  2:914464/1‑140 (MQ=255)

                                                                                  cGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGc                                                 <  1:753105/140‑1 (MQ=255)

                                                                                  cGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGc                                                 <  1:859679/140‑1 (MQ=255)

                                                                                    aTGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAg                                               <  2:893825/140‑1 (MQ=255)

                                                                                     tGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAgg                                              <  2:631174/140‑1 (MQ=255)

                                                                                       aCAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTc                                            <  1:914464/140‑1 (MQ=255)

                                                                                         aGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCtt                                          >  1:1215438/1‑140 (MQ=255)

                                                                                            aGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACg                                       <  1:1096459/140‑1 (MQ=255)

                                                                                            aGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACg                                       <  1:904131/140‑1 (MQ=255)

                                                                                                atgaCCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTAcc                                   >  1:207165/1‑140 (MQ=255)

                                                                                                 tgaCCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTAccc                                  >  2:960371/1‑140 (MQ=255)

                                                                                                 tgaCCTCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTAccc                                  >  1:454484/1‑140 (MQ=255)

                                                                                                      tCAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAg                             <  1:698501/140‑1 (MQ=255)

                                                                                                       cAAAGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGt                            <  2:1215438/140‑1 (MQ=255)

                                                                                                         aaGCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCg                          <  2:592157/140‑1 (MQ=255)

                                                                                                           gCTATCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGcc                        <  1:960371/140‑1 (MQ=255)

                                                                                                              aTCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGa                     <  2:454484/140‑1 (MQ=255)

                                                                                                               tCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGAt                    <  2:338107/140‑1 (MQ=255)

                                                                                                               tCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGAt                    >  1:147719/1‑140 (MQ=255)

                                                                                                               tCAGTAAATTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGAt                    >  1:491526/1‑140 (MQ=255)

                                                                                                                      aTTTAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCg             <  1:160257/140‑1 (MQ=255)

                                                                                                                       tttAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGa            >  1:761672/1‑140 (MQ=255)

                                                                                                                         tAAGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAgg          >  2:1042853/1‑140 (MQ=255)

                                                                                                                           aGGACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGt        <  1:300412/140‑1 (MQ=255)

                                                                                                                            ggACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTg       <  2:761672/140‑1 (MQ=255)

                                                                                                                            ggACCTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTg       <  2:207165/140‑1 (MQ=255)

                                                                                                                                cTCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAg   >  2:681255/1‑140 (MQ=255)

                                                                                                                                 tCACACCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAGt  >  2:580671/1‑140 (MQ=255)

CTGATTTCAGGGAAAAAATAATGAAACGTAAAACACTACCTCTGCTGGCGCTGGTTGCCACCTCTCTGTTTCTGAGCGCCTGCGATGACAGGAGTGATGACCTCAAAGCTATCAGTAAATTTAAGGACCTCACGCCCCCGCGCTTCAGCGATGTGGTCAGCCGTCAGGATGATGTCAGCGAAGAATGGTCACAGGTTGGCTTCTCATCCGGTCTCACCCTGCAGGTCTTACGTACCCGTGAGTCGCCCGATGGTTGCGAGGGTGGCAGT  >  NC_000913/2770661‑2770929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A