Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 129 74.0 2524844 99.2% 2504645 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,060,960 G→A P37P (CCG→CCA)  scpA → methylmalonyl‑CoA mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,060,9600GA97.4% 252.2 / ‑2.4 76P37P (CCG→CCA) scpAmethylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (37/37);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

TTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3060824‑3061095
                                                                                                                                        |                                                                                                                                       
tttGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTg                                                                                                                                      <  2:71072/140‑1 (MQ=255)
  tGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGta                                                                                                                                    >  2:596374/1‑140 (MQ=255)
    aTTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGtata                                                                                                                                  >  2:1207469/1‑140 (MQ=255)
    aTTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGtata                                                                                                                                  >  2:277465/1‑140 (MQ=255)
     ttGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATAc                                                                                                                                 <  2:354776/140‑1 (MQ=255)
        cGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGa                                                                                                                              >  1:144631/1‑140 (MQ=255)
             tACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCg                                                                                                                         <  2:1211194/140‑1 (MQ=255)
                    cAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGa                                                                                                                  >  2:622796/1‑140 (MQ=255)
                           tGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTg                                                                                                           <  1:622796/140‑1 (MQ=255)
                            gTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  2:1203937/1‑140 (MQ=255)
                            gTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  2:217150/1‑140 (MQ=255)
                            gTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  1:732417/1‑140 (MQ=255)
                              ctgaCGAGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAg                                                                                                        >  2:1142766/4‑140 (MQ=255)
                              cTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          <  2:499734/138‑1 (MQ=255)
                              cTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTgg                                                                                                          >  1:499734/1‑138 (MQ=255)
                               tAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAgg                                                                                                       >  2:1146282/1‑140 (MQ=255)
                               tAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAgg                                                                                                       <  1:596374/140‑1 (MQ=255)
                                aaCGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGt                                                                                                      <  1:597677/140‑1 (MQ=255)
                                  cGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGa                                                                                                    >  1:1086761/1‑140 (MQ=255)
                                  cGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGa                                                                                                    >  2:44029/1‑140 (MQ=255)
                                   gTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGAc                                                                                                   <  2:144631/140‑1 (MQ=255)
                                    tGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACa                                                                                                  >  1:124249/1‑140 (MQ=255)
                                      cAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAgg                                                                                                <  2:518166/140‑1 (MQ=255)
                                       aGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGAc                                                                                                   >  2:139680/1‑136 (MQ=255)
                                       aGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGAc                                                                                                   <  1:139680/136‑1 (MQ=255)
                                              gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGt                                                                                                      <  2:1205100/126‑1 (MQ=255)
                                              gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGt                                                                                                      >  1:1205100/1‑126 (MQ=255)
                                              gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:112475/1‑140 (MQ=255)
                                              gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:865839/1‑140 (MQ=255)
                                              gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:344008/1‑140 (MQ=255)
                                              gCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTc                                                                                        >  1:630895/1‑140 (MQ=255)
                                                     cTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGttt                                                                                 >  2:767607/1‑140 (MQ=255)
                                                       tGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgc                                                                               >  1:908916/1‑140 (MQ=255)
                                                       tGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgc                                                                               >  1:576477/1‑140 (MQ=255)
                                                        gCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgcc                                                                              >  2:967150/1‑140 (MQ=255)
                                                          caacaaGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTgccgc                                                                            <  2:576477/140‑1 (MQ=255)
                                                                       aGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTgg                                                               >  1:127928/1‑140 (MQ=255)
                                                                        gCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGc                                                              <  1:661063/140‑1 (MQ=255)
                                                                        gCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGc                                                              >  2:1260993/1‑140 (MQ=255)
                                                                         ccGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGcc                                                             >  1:657673/1‑140 (MQ=255)
                                                                            tCGGGAGAAACCTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTCACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGCGGCCcgc                                                          <  2:274902/140‑1 (MQ=255)
                                                                            tCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgc                                                          >  1:97507/1‑140 (MQ=255)
                                                                            tCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgc                                                          >  1:595219/1‑140 (MQ=255)
                                                                             cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  1:1203937/140‑1 (MQ=255)
                                                                             cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  2:112475/140‑1 (MQ=255)
                                                                             cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  1:885268/140‑1 (MQ=255)
                                                                             cGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCcgcg                                                         <  2:128095/140‑1 (MQ=255)
                                                                               ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  2:344008/140‑1 (MQ=255)
                                                                               ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  1:277465/140‑1 (MQ=255)
                                                                               ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  2:509757/140‑1 (MQ=255)
                                                                               ggAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTg                                                       <  1:767607/140‑1 (MQ=255)
                                                                                 agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  2:963701/140‑1 (MQ=255)
                                                                                 agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  2:657673/140‑1 (MQ=255)
                                                                                 agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  1:967150/140‑1 (MQ=255)
                                                                                 agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  1:1260993/140‑1 (MQ=255)
                                                                                 agaAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGcc                                                     <  1:1207469/140‑1 (MQ=255)
                                                                                    aaaCTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACt                                                  <  2:124249/140‑1 (MQ=255)
                                                                                         gTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGta                                             <  2:908916/140‑1 (MQ=255)
                                                                                             aCTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATAcc                                         >  2:161397/1‑140 (MQ=255)
                                                                                                cGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGcc                                      <  2:127928/140‑1 (MQ=255)
                                                                                                cGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGcc                                      <  2:732417/140‑1 (MQ=255)
                                                                                                cGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGcc                                      <  1:729346/140‑1 (MQ=255)
                                                                                                  cTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCa                                    >  1:945348/1‑140 (MQ=255)
                                                                                                    ggTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAAc                                  >  2:1258703/1‑140 (MQ=255)
                                                                                                        catcaAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTg                              >  1:852690/1‑140 (MQ=255)
                                                                                                        catcaAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTg                              <  2:97507/140‑1 (MQ=255)
                                                                                                         atcaAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTgg                             >  2:1261352/1‑140 (MQ=255)
                                                                                                           caAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAc                           <  1:1258703/140‑1 (MQ=255)
                                                                                                           caAACCGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGAc                           <  1:44029/140‑1 (MQ=255)
                                                                                                               ccGCGGAAGGGATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATc                       <  1:217150/140‑1 (MQ=255)
                                                                                                                        ggATCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTAt              <  1:161397/140‑1 (MQ=255)
                                                                                                                          aTCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGc            >  2:336419/1‑140 (MQ=255)
                                                                                                                           tCGCCATCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCt           <  2:852690/140‑1 (MQ=255)
                                                                                                                                 tCAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGtttt     >  2:16428/1‑140 (MQ=255)
                                                                                                                                  cAAGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGttttt    >  1:466658/1‑140 (MQ=255)
                                                                                                                                   aaGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTc   <  2:595219/140‑1 (MQ=255)
                                                                                                                                    aGCCACTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCa  >  2:1154925/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TTTGATTGCGTTTTACGGAGCAAATAATGTCTAACGTGCAGGAGTGGCAACAGCTTGCCAACAAGGAATTGAGCCGTCGGGAGAAAACTGTCGACTCGCTGGTTCATCAAACCGCGGAAGGGATCGCCATCAAGCCGCTGTATACCGAAGCCGATCTCGATAATCTGGAGGTGACAGGTACCCTTCCTGGTTTGCCGCCCTACGTTCGTGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCA  >  NC_000913/3060824‑3061095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A