Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	129	74.0	2524844	99.2%	2504645	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)_7→8	coding (1041/1245 nt)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	100.0%	61.7 / NA	19	A345G (GCG→GGG)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (2/17); total (2/17)

AATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGA  >  NC_000913/3240838‑3241105

aaTATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTCCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGc                                                                                                                                   <  1:238188/140‑1 (MQ=255)

 atatGGCGGGCGCAGCAATCTCTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                                                                                                                 <  1:472005/140‑1 (MQ=255)

         ggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggt                                                                                                                         <  1:959724/140‑3 (MQ=255)

            gcagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑ggggggggtctc                                                                                                                      <  1:1137374/140‑6 (MQ=255)

                   tCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgct                                                                                                                >  1:87340/1‑140 (MQ=255)

                   tCACTATTACAGTGCTGACGCTGGCTGCGGATAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTctgctgct                                                                                                                <  2:773572/140‑1 (MQ=255)

                          tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                         <  2:266642/140‑1 (MQ=255)

                          tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCa                                                                                                         <  2:821891/140‑1 (MQ=255)

                           aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCAc                                                                                                        >  2:557709/1‑140 (MQ=255)

                              gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTgg                                                                                                     >  2:574350/1‑140 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGc                                                                                                    <  1:35614/140‑1 (MQ=255)

                               tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGc                                                                                                    >  1:785542/1‑140 (MQ=255)

                                 ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCt                                                                                                  >  1:1120601/1‑140 (MQ=255)

                                 ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCt                                                                                                  >  1:281320/1‑140 (MQ=255)

                                   gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGt                                                                                                >  2:248956/1‑140 (MQ=255)

                                    aCGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                                               <  1:557709/140‑1 (MQ=255)

                                      gctggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAat                                                                                             <  1:869129/140‑1 (MQ=255)

                                           ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGtt                                                                                        <  2:778431/140‑1 (MQ=255)

                                                    aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCCGTAATATGTTTGGGAtttt                                                                               >  2:884114/1‑139 (MQ=255)

                                                     tACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCg                                                                              <  2:362092/140‑1 (MQ=255)

                                                       cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                            <  1:574350/140‑1 (MQ=255)

                                                       cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                            <  1:325177/140‑1 (MQ=255)

                                                       cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                            <  1:884114/140‑1 (MQ=255)

                                                          tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                         <  2:1120601/140‑1 (MQ=255)

                                                               aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGGTGATTCCCCTGGCCTGTAATATGTTCGGGATTTCGAACGATATCg                                                                    >  2:1003030/1‑140 (MQ=255)

                                                                                  cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                 <  1:111106/140‑1 (MQ=255)

                                                                                    gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg                                               <  2:281320/140‑1 (MQ=255)

                                                                                             ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatca                                      >  1:957690/1‑140 (MQ=255)

                                                                                                aGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCg                                   <  1:163476/140‑1 (MQ=255)

                                                                                                  cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTTCTGCTTATCCCACTGGCCCGTAATATTTTTGGGATTTCGAAAGATATCGCCATGCAGGGGGTTGCCCTCGGCTTTTTCATCGGc                                 >  2:142904/1‑140 (MQ=255)

                                                                                                   gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                <  2:110395/140‑1 (MQ=255)

                                                                                                    tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCATGCAGGGGGGTGCCGGCGGGTTTTTCATCGGCgg                               >  2:163476/1‑139 (MQ=255)

                                                                                                       tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCCCCGGCCCGTAAAATGTTTGGGATTTCGAACGATATCGCCCTGCAGGGGGGTGCCGTCGGCTTTTTCATCCGCGGAtt                            >  2:874802/1‑140 (MQ=255)

                                                                                                                   gtgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCCCTGGCCTGTAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTg                >  2:494891/1‑140 (MQ=255)

                                                                                                                    tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCCCCGGCCCGGAATATGTTCGGGATTTTCAACGATATCGCCCTGCCGGGGGGTGGCGGCCGGTTTTTTATCGGCGGATTTCAGGGCTCtttc               >  2:796131/1‑138 (MQ=255)

                                                                                                                    tgCCTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGGGGGTGCCGTCGGCTTTATCATCGGCGGATTGCAGGACTCtttc               >  2:328104/1‑138 (MQ=255)

                                                                                                                      ccTGTGGCGCATCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGTAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGGGGGTGCCGTTGGCTTTTTCATCGGCGGATTTCAGGACTCTTGCGa             >  2:863505/1‑140 (MQ=255)

                                                                                                                        tgtgGCGCATCCGGCGTGGCGGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaaa           <  1:425831/140‑1 (MQ=255)

                                                                                                                                 tCCGGCGTGGCGGGGGGGGGCTCTGCTGCTGATCCCACCGGCCTGTAATATGTTCGGGATTTCGGACGATATCGCCATTCAGGGGGTTGGCCTCGGCTTTATCATCGGCGGATTTCAGGACTCTTGCGAAAACgcgccgg  >  2:914990/1‑137 (MQ=255)

AATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑GGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGA  >  NC_000913/3240838‑3241105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A