Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	126	206.4	6980590	99.2%	6924745	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,280,298	(G)_5→6	coding (82/474 nt)	agaV →	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,280,293	1	.	G	98.0%	719.4 / 4.4	203	coding (77/474 nt)	agaV	N‑acetyl‑D‑galactosamine specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (94/105); total (95/108)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 236 total aligned reads displayed.

ACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑GGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACA  >  NC_000913/3280154‑3280421

 cATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑g                                                                                                                                  <  2:2191185/140‑1 (MQ=255)

  aTCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑gg                                                                                                                                 >  1:422190/1‑140 (MQ=255)

  aTCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑gg                                                                                                                                 >  2:2866231/1‑140 (MQ=255)

    ccTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑g                                                                                                                              >  1:362545/1‑140 (MQ=255)

    ccTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑g                                                                                                                              >  1:1164603/1‑140 (MQ=255)

    ccTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑g                                                                                                                              >  1:3416039/1‑140 (MQ=255)

    ccTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑g                                                                                                                              >  2:2805343/1‑140 (MQ=255)

    ccTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑gag‑g                                                                                                                              >  2:1036340/1‑140 (MQ=255)

      tGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑ggg                                                                                                                            <  1:1627662/140‑2 (MQ=255)

       ggCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑gggc                                                                                                                           <  1:1227582/140‑3 (MQ=255)

          gcAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑gggcaaa                                                                                                                        <  1:1766576/140‑6 (MQ=255)

              tACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTg                                                                                                                     >  1:867622/1‑140 (MQ=255)

              tACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTg                                                                                                                     >  1:989228/1‑140 (MQ=255)

              tACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTg                                                                                                                     >  2:2700139/1‑140 (MQ=255)

              tACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑ggg                                                                                                                            <  2:1127756/132‑2 (MQ=255)

                   cacaGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑ggg‑gggc                                                                                                                           <  2:502545/128‑3 (MQ=255)

                    acaGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTg                                                                                                               <  2:1862391/140‑1 (MQ=255)

                        ccTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAg                                                                                                           >  2:1017178/1‑140 (MQ=255)

                        ccTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAg                                                                                                           >  1:2075776/1‑140 (MQ=255)

                        ccTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAg                                                                                                           >  2:3457479/1‑140 (MQ=255)

                        ccTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAg                                                                                                           >  1:1143572/1‑140 (MQ=255)

                         cTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGc                                                                                                          <  1:1563491/140‑1 (MQ=255)

                         cTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGc                                                                                                          <  2:2363145/140‑1 (MQ=255)

                          tgtgAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGcc                                                                                                         >  1:3372232/1‑140 (MQ=255)

                          tgtgAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGcc                                                                                                         <  1:2751051/140‑1 (MQ=255)

                          tgtgAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGc                                                                                                          >  2:1738752/1‑139 (MQ=255)

                           gtgAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCa                                                                                                        >  1:2148322/1‑140 (MQ=255)

                           gtgAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCa                                                                                                        >  2:393107/1‑140 (MQ=255)

                                ggCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGAt                                                                                                   >  2:1921118/1‑140 (MQ=255)

                                  ccAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGa                                                                                                 >  2:1810340/1‑140 (MQ=255)

                                        aGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGc                                                                                           <  1:2546344/140‑1 (MQ=255)

                                           aaaaCAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGa                                                                                        >  2:1579038/1‑140 (MQ=255)

                                           aaaaCAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGa                                                                                        >  2:557182/1‑140 (MQ=255)

                                             aaCAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAg                                                                                      >  1:1119987/1‑140 (MQ=255)

                                                  agagGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCg                                                                                 >  2:288600/1‑140 (MQ=255)

                                                    agGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGt                                                                               >  1:1851224/1‑140 (MQ=255)

                                                    agGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGt                                                                               <  1:1382660/140‑1 (MQ=255)

                                                        acacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTacaa                                                                           >  2:83618/1‑140 (MQ=255)

                                                        acacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTacaa                                                                           <  2:1840043/140‑1 (MQ=255)

                                                         cacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATc                                                                                   >  1:1666362/1‑131 (MQ=255)

                                                         cacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATc                                                                                   <  2:1666362/131‑1 (MQ=255)

                                                         cacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTacaac                                                                          >  1:683144/1‑140 (MQ=255)

                                                         cacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTacaac                                                                          >  1:1011041/1‑140 (MQ=255)

                                                         cacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTacaac                                                                          >  1:1077869/1‑140 (MQ=255)

                                                         cacGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTacaac                                                                          <  2:3209573/140‑1 (MQ=255)

                                                          acGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTacaaca                                                                         >  2:1194729/1‑140 (MQ=255)

                                                              tATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAAc                                                                     >  2:152779/1‑140 (MQ=255)

                                                              tATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAAc                                                                     <  1:1810340/140‑1 (MQ=255)

                                                               aTGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAAcc                                                                    <  2:867622/140‑1 (MQ=255)

                                                                tGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑GGGCGGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAGCCt                                                                   >  2:725241/1‑140 (MQ=255)

                                                                 gCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTg                                                                  >  2:641155/1‑140 (MQ=255)

                                                                  ccAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGa                                                                 >  1:1916119/1‑140 (MQ=255)

                                                                   cAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGAt                                                                <  1:949584/140‑1 (MQ=255)

                                                                   cAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGAt                                                                <  2:2130520/140‑1 (MQ=255)

                                                                    aaaTATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATg                                                               <  1:839861/140‑1 (MQ=255)

                                                                       tatTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGaa                                                            <  1:2878649/140‑1 (MQ=255)

                                                                        atTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGaaa                                                           <  1:80978/140‑1 (MQ=255)

                                                                         ttGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAAt                                                          <  2:1164603/140‑1 (MQ=255)

                                                                         ttGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAAt                                                          <  2:2418846/140‑1 (MQ=255)

                                                                         ttGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAAt                                                          <  2:2779608/140‑1 (MQ=255)

                                                                          tGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACaaa                                                                       >  1:2909010/1‑126 (MQ=255)

                                                                            ttttAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGAt                                                                >  2:2375480/1‑131 (MQ=255)

                                                                            ttttAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGAt                                                                <  1:2375480/131‑1 (MQ=255)

                                                                            ttttAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGt                                                       >  2:298071/1‑140 (MQ=255)

                                                                            ttttAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGt                                                       >  2:200818/1‑140 (MQ=255)

                                                                              ttAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTAc                                                     <  1:1733971/140‑1 (MQ=255)

                                                                              ttAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTAc                                                     <  1:3362482/140‑1 (MQ=255)

                                                                                 aGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTAc                                                     <  2:1435993/137‑1 (MQ=255)

                                                                                 aGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTAc                                                     >  1:1435993/1‑137 (MQ=255)

                                                                                 aGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTgg                                                  >  2:966033/1‑140 (MQ=255)

                                                                                 aGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTgg                                                  <  2:2075776/140‑1 (MQ=255)

                                                                                 aGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTgg                                                  >  1:2646069/1‑140 (MQ=255)

                                                                                     ggATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGa                                              <  1:200818/140‑1 (MQ=255)

                                                                                        ttGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAgg                                           <  1:963713/140‑1 (MQ=255)

                                                                                        ttGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAgg                                           <  1:2815337/140‑1 (MQ=255)

                                                                                            tgaACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGAt                                        <  2:1361495/139‑1 (MQ=255)

                                                                                               aCGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGcc                                    <  1:3130837/140‑1 (MQ=255)

                                                                                                cGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTAc                                                     <  2:1229427/122‑1 (MQ=255)

                                                                                                cGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCg                                   >  2:2491391/1‑140 (MQ=255)

                                                                                                  cTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTa                                 <  2:954320/140‑1 (MQ=255)

                                                                                                  cTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTa                                 <  1:641155/140‑1 (MQ=255)

                                                                                                  cTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTa                                 <  2:3416039/140‑1 (MQ=255)

                                                                                                  cTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTa                                 <  2:300036/140‑1 (MQ=255)

                                                                                                    tGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTa                                 >  2:2060886/1‑138 (MQ=255)

                                                                                                           cGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTa                                 >  2:309762/1‑131 (MQ=255)

                                                                                                           cGGTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTa                                 <  1:309762/131‑1 (MQ=255)

                                                                                                             gTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGAc                      <  1:2491391/140‑1 (MQ=255)

                                                                                                             gTCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGAc                      <  1:2356140/140‑1 (MQ=255)

                                                                                                              tCAGGTCGGCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACg                     <  1:2799835/140‑1 (MQ=255)

                                                                                                                     ggCGTTCAATGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCaaa              >  1:3033410/1‑140 (MQ=255)

                                                                                                                            aaTGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTAt       <  1:298071/140‑1 (MQ=255)

                                                                                                                              tGGGTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCg     <  1:2069086/140‑1 (MQ=255)

                                                                                                                                ggTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGac   <  2:3372232/140‑1 (MQ=255)

                                                                                                                                 gTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCTGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGac   <  1:1245589/139‑1 (MQ=255)

                                                                                                                                 gTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCTGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGac   >  2:1245589/1‑139 (MQ=255)

                                                                                                                                 gTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTAt       >  1:573295/1‑135 (MQ=255)

                                                                                                                                 gTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGaca  >  2:2742727/1‑140 (MQ=255)

                                                                                                                                 gTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGaca  >  2:2604325/1‑140 (MQ=255)

                                                                                                                                 gTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGaca  <  1:1284198/140‑1 (MQ=255)

                                                                                                                                 gTCGGATTTGCGGGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGaca  >  1:1035579/1‑140 (MQ=255)

ACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCAATGGGTCGGATTTGC‑GGG‑GGCAAATCTGGTGCTGGTAGCCAACGATGAGGTTGCCGAAGATCCGGTACAACAAAACCTGATGGAAATGGTACTGGCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACA  >  NC_000913/3280154‑3280421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A