Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	126	206.4	6980590	99.2%	6924745	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,314	T→C	L151P (CTC→CCC)	yhhZ →	putative endonuclease YhhZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,314	0	T	C	99.6%	1011.7 / NA	279	L151P (CTC→CCC)	yhhZ	putative endonuclease YhhZ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (153/124); minor base A (0/1); total (153/126)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

CTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGA  >  NC_000913/3582178‑3582447

cTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACcccc                                                                                                                                    >  2:1410953/1‑140 (MQ=255)

cTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACcccc                                                                                                                                    >  1:459027/1‑140 (MQ=255)

cTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACcccc                                                                                                                                    >  2:1617100/1‑140 (MQ=255)

   agagGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGa                                                                                                                                 >  1:780463/1‑140 (MQ=255)

        cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCAcccc                                                                                                                                     >  2:2706018/1‑131 (MQ=255)

        cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAAtt                                                                                                                            >  2:1213597/1‑140 (MQ=255)

        cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAAtt                                                                                                                            >  1:134005/1‑140 (MQ=255)

        cgcTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAAtt                                                                                                                            >  2:1999076/1‑140 (MQ=255)

          cTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATtat                                                                                                                          <  2:459027/140‑1 (MQ=255)

                tATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCgt                                                                                                                    <  1:3045970/140‑1 (MQ=255)

                tATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCgt                                                                                                                    <  2:2408448/140‑1 (MQ=255)

                     gCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCgtttgtt                                                                                                               >  2:2165417/1‑140 (MQ=255)

                       tATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGtttt                                                                                                             >  2:878806/1‑140 (MQ=255)

                         ttCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCgt                                                                                                                    <  1:2577097/131‑1 (MQ=255)

                               aCCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAAt                                                                                                     >  2:2988668/1‑140 (MQ=255)

                                ccAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAAtt                                                                                                    >  2:1650019/1‑140 (MQ=255)

                                   gATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaa                                                                                                 >  2:170656/1‑140 (MQ=255)

                                   gATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaa                                                                                                 >  2:3087461/1‑140 (MQ=255)

                                   gATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaa                                                                                                 >  2:1872398/1‑140 (MQ=255)

                                   gATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCaa                                                                                                 >  2:2329042/1‑140 (MQ=255)

                                         tGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAAcc                                                                                           >  1:2261003/1‑140 (MQ=255)

                                          gAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACca                                                                                          <  2:421814/140‑1 (MQ=255)

                                           aaaaCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaa                                                                                         >  1:1459178/1‑140 (MQ=255)

                                           aaaaCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaa                                                                                         >  1:313908/1‑140 (MQ=255)

                                              aCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaaca                                                                                      >  1:2209268/1‑140 (MQ=255)

                                              aCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaaca                                                                                      >  2:149510/1‑140 (MQ=255)

                                              aCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaaca                                                                                      >  2:3327957/1‑140 (MQ=255)

                                              aCCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACcaaaca                                                                                      >  1:2933462/1‑140 (MQ=255)

                                                   cTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaataa                                                                                 >  2:358606/1‑140 (MQ=255)

                                                   cTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaataa                                                                                 >  2:1235977/1‑140 (MQ=255)

                                                    tGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAaataat                                                                                <  2:530610/140‑1 (MQ=255)

                                                             aaaCAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCaaa                                                                       >  2:799861/1‑140 (MQ=255)

                                                                cAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACg                                                                    <  2:210836/140‑1 (MQ=255)

                                                                     aCTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTaaa                                                               >  1:2479036/1‑140 (MQ=255)

                                                                       tATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACa                                                             >  2:1288378/1‑140 (MQ=255)

                                                                          tAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCa                                                          <  1:1716805/140‑1 (MQ=255)

                                                                          tAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCa                                                          <  1:2329042/140‑1 (MQ=255)

                                                                          tAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCa                                                          <  1:3458196/140‑1 (MQ=255)

                                                                          tAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCa                                                          <  1:522765/140‑1 (MQ=255)

                                                                           aGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCa                                                                         <  2:2731782/124‑1 (MQ=255)

                                                                               aTGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCt                                                     >  1:2714912/1‑140 (MQ=255)

                                                                               aTGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCt                                                     >  1:590227/1‑140 (MQ=255)

                                                                               aTGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCt                                                     >  1:149218/1‑140 (MQ=255)

                                                                                tGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTa                                                    <  2:3394741/140‑1 (MQ=255)

                                                                                 gAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTAt                                                   >  2:1016839/1‑140 (MQ=255)

                                                                                    tttATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTgg                                                <  1:866956/140‑1 (MQ=255)

                                                                                     ttATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGc                                               <  1:3327957/140‑1 (MQ=255)

                                                                                       aTCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAg                                             >  1:3386420/1‑140 (MQ=255)

                                                                                       aTCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAg                                             <  2:2021313/140‑1 (MQ=255)

                                                                                        tCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAgg                                            <  1:2633986/140‑1 (MQ=255)

                                                                                            ctGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCt                                                     >  2:2834268/1‑127 (MQ=255)

                                                                                             tGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGc                                               <  1:38554/132‑1 (MQ=255)

                                                                                             tGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACt                                       >  1:115772/1‑140 (MQ=255)

                                                                                             tGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACt                                       <  1:1016839/140‑1 (MQ=255)

                                                                                               tCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTtg                                     >  2:2598688/1‑140 (MQ=255)

                                                                                               tCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTtg                                     >  2:1929347/1‑140 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  1:81046/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  2:780463/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  1:1929347/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  1:1980373/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  2:2284720/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  1:2370563/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  2:1968851/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  1:90606/140‑1 (MQ=255)

                                                                                                     tCTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGctgctg                               <  1:3087461/140‑1 (MQ=255)

                                                                                                      cTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtgg                              >  2:1767053/1‑140 (MQ=255)

                                                                                                      cTTATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtgg                              >  1:1687887/1‑140 (MQ=255)

                                                                                                       ttATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAGACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggt                             <  1:2684186/140‑1 (MQ=255)

                                                                                                       ttATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggt                             <  1:1767053/140‑1 (MQ=255)

                                                                                                        tATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggtg                            <  2:2828865/140‑1 (MQ=255)

                                                                                                        tATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggtg                            <  2:310987/140‑1 (MQ=255)

                                                                                                        tATCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggtg                            <  2:1687887/140‑1 (MQ=255)

                                                                                                         aTCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggtgg                           >  1:2310603/1‑140 (MQ=255)

                                                                                                         aTCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggtgg                           >  2:3418648/1‑140 (MQ=255)

                                                                                                         aTCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggtgg                           >  1:655163/1‑140 (MQ=255)

                                                                                                         aTCGCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggt                             >  2:208906/1‑138 (MQ=255)

                                                                                                            gCAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGt                        <  1:2755056/140‑1 (MQ=255)

                                                                                                             cAAATACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGta                       <  1:2104558/140‑1 (MQ=255)

                                                                                                              aaaTACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGtat                      <  2:2914095/140‑1 (MQ=255)

                                                                                                                aTACCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATAc                    >  1:2155502/1‑140 (MQ=255)

                                                                                                                  aCCGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACaa                  <  1:2842663/140‑1 (MQ=255)

                                                                                                                   ccGAGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAAt                 <  1:3418648/140‑1 (MQ=255)

                                                                                                                      aGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGta                       <  1:3350137/131‑1 (MQ=255)

                                                                                                                      aGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATggg              >  2:182628/1‑140 (MQ=255)

                                                                                                                      aGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATggg              <  1:1587345/140‑1 (MQ=255)

                                                                                                                      aGTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCG‑‑TACAATGGGaa            >  2:2522365/1‑140 (MQ=255)

                                                                                                                       gTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGa             <  1:201795/140‑1 (MQ=255)

                                                                                                                       gTTCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGa             >  1:1405407/1‑140 (MQ=255)

                                                                                                                        ttCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCtggt                             >  1:2068211/1‑123 (MQ=255)

                                                                                                                        ttCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGaa            >  1:2821302/1‑140 (MQ=255)

                                                                                                                        ttCAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTC‑AAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAAc           >  2:597353/1‑140 (MQ=255)

                                                                                                                          cAGCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACa          >  2:320534/1‑140 (MQ=255)

                                                                                                                            gCTATTTGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACAtt        >  2:2655238/1‑140 (MQ=255)

                                                                                                                                tttGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAg    <  2:2310603/140‑1 (MQ=255)

                                                                                                                                tttGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAg    <  2:1330699/140‑1 (MQ=255)

                                                                                                                                tttGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAg    <  1:1639571/140‑1 (MQ=255)

                                                                                                                                tttGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATCGAAg    <  1:534722/140‑1 (MQ=255)

                                                                                                                                  tGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGa  <  1:1876514/140‑1 (MQ=255)

                                                                                                                                  tGGCACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGa  >  1:1897048/1‑140 (MQ=255)

                                                                                                                                     cACCCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGa  <  2:2955573/137‑1 (MQ=255)

CTAAGAGGCGCTTTTTTATCGGCTATTCATCACCAGATCATTGAAAACCAACTGGATACAGAAACAATAACTATTAGTTATGAATTTATCCTCTGTCAACATCTTATCGCAAATACCGAGTTCAGCTATTTGGCACTCCCTGAAAATTATAACCGTTTGTTTTTACCAAATTCAAAAAACCAAACAAATAATCGTTTCAAAACGTTAAACAGCAAAGCTATTGGCAGGCTACTTGCTGCTGGTGGCGTATACAATGGGAACATTGAAGGA  >  NC_000913/3582178‑3582447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A