Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F28 I1 R1
|
252 |
48.5 |
1661298 |
98.8% |
1641362 |
138.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,690,148 |
T→C |
G99G (GGT→GGC) |
ydgA → |
conserved protein YdgA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,690,148 | 0 | T | C | 100.0%
| 83.0
/ NA
| 25 | G99G (GGT→GGC) | ydgA | conserved protein YdgA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/16); total (9/16) |
AGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCTTT > NC_000913/1690017‑1690273
|
aGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTccc > 1:172395/1‑140 (MQ=255)
aGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTccc > 1:297914/1‑140 (MQ=255)
ttATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGc < 2:145420/140‑1 (MQ=255)
cAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGc < 1:98726/140‑1 (MQ=255)
aaaaCTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGcc < 1:740298/140‑1 (MQ=255)
aaaaCTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGcc < 1:763093/140‑1 (MQ=255)
tcGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGGGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTaaaa > 2:412843/1‑140 (MQ=255)
cgTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTCGCCCAGCTTAAAAAACTGa < 1:297171/140‑1 (MQ=255)
gTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGaa > 1:605955/1‑140 (MQ=255)
aaCTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGGATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCa < 1:783442/140‑1 (MQ=255)
aaaCCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAAc < 2:20055/140‑1 (MQ=255)
aCCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCa > 2:290496/1‑140 (MQ=255)
ccATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACg < 2:559649/140‑1 (MQ=255)
ccATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACg < 1:489350/140‑1 (MQ=255)
gggAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCAGTCGATGGCATCAATTCAAACCACGCTGGTtaa > 1:97005/1‑140 (MQ=255)
aaagaaaATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTtaataa < 1:220202/140‑1 (MQ=255)
ccGTGGATTAAAAGCGGTCAGGGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAg < 1:412843/140‑1 (MQ=255)
gATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAAc < 1:667708/140‑1 (MQ=255)
ttAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCa < 2:293595/140‑1 (MQ=255)
gTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGat > 2:790793/1‑140 (MQ=255)
cAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCa > 2:322730/1‑129 (MQ=255)
cAGAGCGTCATCTTCAACGAATCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCa < 1:322730/129‑1 (MQ=255)
aTCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCtt > 2:450959/1‑140 (MQ=255)
tCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCttt < 1:290496/140‑1 (MQ=255)
tCGGTTGATCATGGCCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCttt < 2:607450/140‑1 (MQ=255)
|
AGTTATCAAAACTATCATCGCGGCGTATTCAGCAGCCAGTTGCAACTGTTGGTGAAACCCATTGCCGGGAAAGAAAATCCGTGGATTAAAAGCGGTCAGAGCGTCATCTTCAACGAATCGGTTGATCATGGTCCCTTCCCGCTTGCCCAGCTTAAAAAACTGAACCTGATCCCGTCGATGGCATCAATTCAAACCACGCTGGTTAATAACGAAGTAAGCAAACCACTGTTTGATATGGCAAAAGGTGAAACGCCTTT > NC_000913/1690017‑1690273
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
