Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 252 48.5 1661298 98.8% 1641362 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,736,876 T→C T139A (ACC→GCC)  ydhP ← putative transporter YdhP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,736,8760TC100.0% 55.6 / NA 17T139A (ACC→GCC) ydhPputative transporter YdhP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/6);  total (11/6)

CACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCAGGCTGGTCAAAATGCGTGAAAGCATCAGGGTCA  >  NC_000913/1736741‑1737010
                                                                                                                                       |                                                                                                                                      
cccttttAGGTAATGAGAAGAACAGATTTCCCATTAAAATCCCCCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAAc                                                                                                                                    <  1:142143/136‑1 (MQ=255)
ccctttgaGGAAATGAAAAAAACAGACTTCCCATAAAAACCCCCCCCACCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAAc                                                                                                                                    <  1:590448/133‑1 (MQ=255)
  ccTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAAccc                                                                                                                                  >  2:408515/1‑140 (MQ=255)
             tGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAg                                                                                                                       >  2:478615/1‑140 (MQ=255)
                              ccATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGcc                                                                                                      >  2:344852/1‑140 (MQ=255)
                                       tCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGt                                                                                             >  2:109802/1‑140 (MQ=255)
                                              cAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGca                                                                                      <  1:658303/140‑1 (MQ=255)
                                               aGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGcac                                                                                     >  2:35688/1‑140 (MQ=255)
                                                     gccgTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTTGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCt                                                                               >  1:182592/1‑140 (MQ=255)
                                                                                         tCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTcc                                           <  2:463166/140‑1 (MQ=255)
                                                                                               aaCCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGAtt                                     >  2:164109/1‑140 (MQ=255)
                                                                                                 ccAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCa                                   >  2:63398/1‑140 (MQ=255)
                                                                                                       cgccgcCGGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCAGGCTgg                             >  1:383608/1‑140 (MQ=255)
                                                                                                             cgGCACGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCAGGCTGGTCaaaa                       <  2:270319/140‑1 (MQ=255)
                                                                                                                  cGCCACCGATATTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCAGGCTGGTCAAAATGCGt                  >  2:42223/1‑140 (MQ=255)
                                                                                                                          atatTTGCCAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCAGGCTGGTCAAAATGCGTGAAAGCAt          <  1:712249/140‑1 (MQ=255)
                                                                                                                                  cAGGGCTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCAGGCTGGTCAAAATGCGTGAAAGCATCAGGGTCa  >  2:356201/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CACCTTTAGGTAATGAGAAGAACAGACTTACCATTGAAATCACTCCCAGCCCCGCCGTTGCCAGAAATGACATCCGCCAGCCGATGGTTTCACCCAACCAGGTCGCCGCCGGCACGCCACCGATATTTGCCAGGGTTAACCCCATAAACATAGTGGCAACTGCGCTGGCCTGTTTATGTTTTGGCACCACGCTTGCGGCCACGACTGAACCCAAACCAAAAAATGCTCCGTGATTCAGGCTGGTCAAAATGCGTGAAAGCATCAGGGTCA  >  NC_000913/1736741‑1737010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A