Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,911,616	T→C	G447G (GGT→GGC)	yebQ →	putative transporter YebQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,911,616	0	T	C	100.0%	128.0 / NA	37	G447G (GGT→GGC)	yebQ	putative transporter YebQ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/19); total (18/19)

CTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGT  >  NC_000913/1911486‑1911750

cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGTTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  2:621933/1‑140 (MQ=255)

cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  2:535808/1‑140 (MQ=255)

cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  2:181372/1‑140 (MQ=255)

cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  1:82829/1‑140 (MQ=255)

cTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATc                                                                                                                               >  1:678123/1‑140 (MQ=255)

   gTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACt                                                                                                                            <  2:82829/140‑1 (MQ=255)

   gTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACt                                                                                                                            <  2:493014/140‑1 (MQ=255)

   gTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACt                                                                                                                            <  1:607737/140‑1 (MQ=255)

        cTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGcc                                                                                                                       <  1:181372/140‑1 (MQ=255)

                 agTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAg                                                                                                              <  2:326675/140‑1 (MQ=255)

                      cggcgcggcgCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCAt                                                                                                         >  2:819868/1‑140 (MQ=255)

                      cggcgcggcgCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCAt                                                                                                         >  1:719811/1‑140 (MQ=255)

                           cggcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCa                                                                                                                      >  2:31177/1‑122 (MQ=255)

                           cggcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCa                                                                                                                      <  1:31177/122‑1 (MQ=255)

                           cggcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATaaaaa                                                                                                    >  1:516764/1‑140 (MQ=255)

                             gcgcTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATaaaaaaa                                                                                                  <  1:819868/140‑1 (MQ=255)

                                 tggtggCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAgcgc                                                                                              <  2:516764/140‑1 (MQ=255)

                                                        tttGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAg                                                                       >  2:610958/1‑140 (MQ=255)

                                                          tGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTa                                                                     >  2:569496/1‑140 (MQ=255)

                                                          tGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTa                                                                     >  2:335502/1‑140 (MQ=255)

                                                            gagaTAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTAtt                                                                   <  2:678123/140‑1 (MQ=255)

                                                              gaTAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTa                                                                 <  1:610958/140‑1 (MQ=255)

                                                                tAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACt                                                               >  1:410315/1‑140 (MQ=255)

                                                                     gTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCata                                                          <  1:621933/140‑1 (MQ=255)

                                                                      tatacacGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCatat                                                         <  1:562664/137‑1 (MQ=255)

                                                                        cacacGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTa                                                       >  2:532203/1‑140 (MQ=255)

                                                                          cacGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACt                                                     >  2:225932/1‑140 (MQ=255)

                                                                            cGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTc                                                   <  1:225932/140‑1 (MQ=255)

                                                                              tctcGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAg                                                 >  1:209390/1‑140 (MQ=255)

                                                                               ctcGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACt                                                               <  2:441607/125‑1 (MQ=255)

                                                                               ctcGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACt                                                               >  1:441607/1‑125 (MQ=255)

                                                                                     gATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCa                                          <  2:209390/140‑1 (MQ=255)

                                                                                           tGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTa                                    >  2:565811/1‑140 (MQ=255)

                                                                                               gCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCa                                <  1:535808/140‑1 (MQ=255)

                                                                                                        gCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCaa                       <  2:316147/140‑1 (MQ=255)

                                                                                                        gCAGTGATTGCTGCCTGTGTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCaa                       <  2:719811/140‑1 (MQ=255)

                                                                                                                       tgtgTCAGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAgcg        >  2:771621/1‑140 (MQ=255)

                                                                                                                             aGTGGCTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCgggt  <  1:565811/140‑1 (MQ=255)

CTCGTCTACTGGGTCAGAGTAGCGGCGCGGCGCTGGTGGCGCTGATGCTAAATCAGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGT  >  NC_000913/1911486‑1911750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A