Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,122,017	T→C	E321E (GAA→GAG)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,122,017	0	T	C	100.0%	147.0 / NA	43	E321E (GAA→GAG)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/23); total (20/23)

GTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATT  >  NC_000913/2121881‑2122148

gTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGtcctcc                                                                                                                                  <  1:576568/140‑1 (MQ=255)

 tACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTcctcct                                                                                                                                 >  2:790640/1‑140 (MQ=255)

  aCCAGTTCGTCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCtt                                                                                                                                >  2:616661/1‑140 (MQ=255)

      gTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTgcgc                                                                                                                            >  2:434198/1‑140 (MQ=255)

           cccAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACg                                                                                                                       <  2:488574/140‑1 (MQ=255)

           cccAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACg                                                                                                                       <  2:301424/140‑1 (MQ=255)

           cccAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACg                                                                                                                       >  1:362997/1‑140 (MQ=255)

            ccAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGt                                                                                                                      >  1:89973/1‑140 (MQ=255)

                cGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCtt                                                                                                                  >  2:702228/1‑140 (MQ=255)

                cGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCtt                                                                                                                  <  2:611019/140‑1 (MQ=255)

                cGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCtt                                                                                                                  <  2:362997/140‑1 (MQ=255)

                        cTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAg                                                                                                          >  2:806551/1‑140 (MQ=255)

                            cAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCgt                                                                                                      >  2:45430/1‑140 (MQ=255)

                                   acCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGt                                                                                               >  2:395377/1‑140 (MQ=255)

                                       gTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTc                                                                                           <  1:64570/140‑1 (MQ=255)

                                          cGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGAc                                                                                        >  2:707986/1‑140 (MQ=255)

                                             cGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACAtt                                                                                     >  1:252153/1‑140 (MQ=255)

                                                ccAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTAcc                                                                                  <  1:45430/140‑1 (MQ=255)

                                                      gcCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTg                                                                            >  1:320701/1‑140 (MQ=255)

                                                             gATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTg                                                                     <  1:800398/140‑1 (MQ=255)

                                                                  tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCa                                                                <  2:406433/140‑1 (MQ=255)

                                                                  tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCa                                                                <  2:320701/140‑1 (MQ=255)

                                                                  tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCa                                                                >  2:794029/1‑140 (MQ=255)

                                                                       cTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCgg                                                                  <  1:345838/133‑1 (MQ=255)

                                                                       cTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCgg                                                                  >  2:345838/1‑133 (MQ=255)

                                                                       cTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTcac                                                           <  2:89973/140‑1 (MQ=255)

                                                                         gTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTcacca                                                         >  1:609301/1‑140 (MQ=255)

                                                                          tCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTcaccac                                                        >  2:594998/1‑140 (MQ=255)

                                                                                 aaGCGAAATCACGGAAGTAATGTTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACg                                                 <  1:694314/140‑1 (MQ=255)

                                                                                  aGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACgg                                                >  2:804396/1‑140 (MQ=255)

                                                                                  aGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACgg                                                <  1:616661/140‑1 (MQ=255)

                                                                                  aGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACgg                                                <  1:395377/140‑1 (MQ=255)

                                                                                          cACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAg                                        <  1:804396/140‑1 (MQ=255)

                                                                                            cGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGga                                      <  2:609301/140‑1 (MQ=255)

                                                                                            cGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGga                                      <  1:434198/140‑1 (MQ=255)

                                                                                               aaGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGgagag                                   <  1:707986/140‑1 (MQ=255)

                                                                                                 gTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGAc                                 <  1:702228/140‑1 (MQ=255)

                                                                                                  tAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTTCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGAcg                                <  1:380556/140‑1 (MQ=255)

                                                                                                  tAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGAcg                                <  1:794029/140‑1 (MQ=255)

                                                                                                  tAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGAcg                                <  1:790640/140‑1 (MQ=255)

                                                                                                          ggCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGga                                      <  2:29881/126‑1 (MQ=255)

                                                                                                          ggCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGga                                      >  1:29881/1‑126 (MQ=255)

                                                                                                          ggCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGAcgtggatcg                         <  2:90562/139‑1 (MQ=255)

                                                                                                          ggCGCTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGAcgtggatcg                         >  1:90562/1‑139 (MQ=255)

                                                                                                            cgcTCATTTCGCCACCATAGATGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGAcgtggatcgtgg                      >  1:696313/1‑140 (MQ=255)

                                                                                                                                aTGGCGTCCTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGAtt  >  1:114312/1‑140 (MQ=255)

GTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATACGTTCTTTAATAAAGGCGTGTCCGGTTTTCGACATTACCGGCGTGCCACCTGCGGCAGTCACCACATCAACGGTGTTCCAGGAGAGACGTGGATCGTGGATGATCTTCGCGCCGGGATT  >  NC_000913/2121881‑2122148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A