Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,246,731	A→G	C13C (TGT→TGC)	cirA ←	ferric dihyroxybenzoylserine outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,246,731	0	A	G	100.0%	120.2 / NA	36	C13C (TGT→TGC)	cirA	ferric dihyroxybenzoylserine outer membrane transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/20); total (16/20)

AGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGG  >  NC_000913/2246594‑2246856

aGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAgca                                                                                                                             <  1:335448/140‑1 (MQ=255)

 ggTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAgcac                                                                                                                            <  1:58444/140‑1 (MQ=255)

                tGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACg                                                                                                             >  1:36161/1‑140 (MQ=255)

                   cGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGaa                                                                                                           >  2:481182/1‑139 (MQ=255)

                   cGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGaa                                                                                                           <  1:481182/139‑1 (MQ=255)

                     cTGATACTGGCAGGCGCATTTTTAAGATTTTGTTCCACCGAAGATGCAGTGACAACCATCGTTTTGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAgg                                                                                                        >  1:764365/1‑140 (MQ=255)

                       gATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGt                                                                                                      <  1:99767/140‑1 (MQ=255)

                        aTACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGtt                                                                                                     <  2:165409/140‑1 (MQ=255)

                              gCAGGTGCCTCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGaaa                                                                                                          >  1:207592/1‑129 (MQ=255)

                              gCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGCCAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCt                                                                                               <  2:764365/140‑1 (MQ=255)

                              gCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGaaa                                                                                                          <  2:207592/129‑1 (MQ=255)

                                  gTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGTCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAAc                                                                                           <  2:514798/140‑1 (MQ=255)

                                   tGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACa                                                                                          <  2:36161/140‑1 (MQ=255)

                                       tCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTcc                                                                                      <  2:305211/140‑1 (MQ=255)

                                       tCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTcc                                                                                      <  1:705498/140‑1 (MQ=255)

                                            aaGATTTTGTTCCACGGAAGATGCAATGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTaaa                                                                                            <  2:146621/129‑1 (MQ=255)

                                            aaGATTTTGTTCCACGGAAGATGCAATGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTaaa                                                                                            >  1:146621/1‑129 (MQ=255)

                                                  ttGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATg                                                                           >  1:670795/1‑140 (MQ=255)

                                                  ttGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATg                                                                           <  2:656391/140‑1 (MQ=255)

                                                   tGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGa                                                                          >  2:189085/1‑140 (MQ=255)

                                                          cGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACt                                                                   >  2:38679/1‑140 (MQ=255)

                                                            gAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTAc                                                                 <  2:670795/140‑1 (MQ=255)

                                                                aTGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaa                                                             <  1:38679/140‑1 (MQ=255)

                                                                      tGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGaaaataaaa                                                       >  2:196790/1‑140 (MQ=255)

                                                                           aCCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGt                                                  >  1:136813/1‑140 (MQ=255)

                                                                           aCCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGt                                                  >  1:492567/1‑140 (MQ=255)

                                                                                 gTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGc                                            <  2:168805/140‑1 (MQ=255)

                                                                                      gCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTcaca                                       <  1:196790/140‑1 (MQ=255)

                                                                                         atcatcatcGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACAtct                                    <  2:492567/140‑1 (MQ=255)

                                                                                         atcatcatcGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACAtct                                    <  2:136813/140‑1 (MQ=255)

                                                                                                   aCCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTc                          <  1:406871/140‑1 (MQ=255)

                                                                                                      gCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTcccc                       >  1:600700/1‑140 (MQ=255)

                                                                                                      gCTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTcccc                       >  2:97378/1‑140 (MQ=255)

                                                                                                       cTAACACAGGCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCt                      >  1:371116/1‑140 (MQ=255)

                                                                                                                gCCATGCACAAGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACgg             <  2:242898/140‑1 (MQ=255)

                                                                                                                          aGAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATcgcg   >  1:518866/1‑140 (MQ=255)

                                                                                                                           gAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCgg  <  1:83920/140‑1 (MQ=255)

                                                                                                                           gAAATAGCGGACAAGCACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCgg  >  2:83920/1‑140 (MQ=255)

AGGTCTTCCTGGGTAATGACGCTGATACTGGCAGGTGCATCTTTAAGATTTTGTTCCACGGAAGATGCAGTGACAACCATCGTTTCGCCATCATCATCGACCGCTAACACAGGCCATGCACAAGAAATAGCGGACAAACACAGCCCGACCCGTACGAAAGGGTTCAACCTAAACATTCCATATCTCCATGAGGTAACTACGAAAATAAAATGGGTTATCGCTCACATCTTCTTCACGTCCCCTTGCGTACGGCAGCATCGCGG  >  NC_000913/2246594‑2246856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A