Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,249,929	T→C	G71G (GGT→GGC)	yeiH →	conserved inner membrane protein YeiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,249,929	0	T	C	97.9%	159.1 / ‑4.3	48	G71G (GGT→GGC)	yeiH	conserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (21/26); total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

TCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTT  >  NC_000913/2249793‑2250063

tCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACggcggt                                                                                                                                     <  1:118043/140‑1 (MQ=255)

tCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACggcggt                                                                                                                                     >  1:579318/1‑140 (MQ=255)

tCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACggcggt                                                                                                                                     >  1:327709/1‑140 (MQ=255)

   ccGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGtgtg                                                                                                                                  <  2:815953/140‑1 (MQ=255)

     ggggTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCt                                                                                                                                <  1:242771/140‑1 (MQ=255)

       ggTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGt                                                                                                                              <  2:696944/140‑1 (MQ=255)

         tCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGgtgt                                                                                                                                   >  2:50716/1‑133 (MQ=255)

         tCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGgtgt                                                                                                                                   <  1:50716/133‑1 (MQ=255)

           gCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGc                                                                                                                          <  1:564724/140‑1 (MQ=255)

             ccTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCa                                                                                                                        <  1:362979/140‑1 (MQ=255)

             ccTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCa                                                                                                                        <  2:676479/140‑1 (MQ=255)

               tgtgGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAg                                                                                                                      <  2:327709/140‑1 (MQ=255)

                gtgGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGc                                                                                                                     <  2:579318/140‑1 (MQ=255)

                gtgGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGc                                                                                                                     <  2:706781/140‑1 (MQ=255)

                         ttCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAAtatttat                                                                                                            <  1:416854/140‑1 (MQ=255)

                         ttCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAAtatttat                                                                                                            >  1:780698/1‑140 (MQ=255)

                           ccATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATttatta                                                                                                          <  2:325091/140‑1 (MQ=255)

                             aTTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACg                                                                                                        >  2:772807/1‑140 (MQ=255)

                                   gcgGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTggg                                                                                                  >  2:8688/1‑140 (MQ=255)

                                        tGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCa                                                                                             >  2:652177/1‑140 (MQ=255)

                                                 cGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATg                                                                                    >  1:181261/1‑140 (MQ=255)

                                                    gTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCt                                                                                 <  1:8688/140‑1 (MQ=255)

                                                                 aCCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCCAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGtt                                                                    >  2:25422/1‑140 (MQ=255)

                                                                      cGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGc                                                               >  2:660298/1‑140 (MQ=255)

                                                                      cGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGc                                                               >  1:116763/1‑140 (MQ=255)

                                                                           tCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAAtt                                                          >  2:109450/1‑140 (MQ=255)

                                                                               gttgGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCg                                                      <  1:652177/140‑1 (MQ=255)

                                                                                   gggATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGt                                                  <  2:116763/140‑1 (MQ=255)

                                                                                   gggATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGt                                                  <  1:772807/140‑1 (MQ=255)

                                                                                     gATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCg                                                >  1:251706/1‑140 (MQ=255)

                                                                                     gATGGTTTTAGGCAACACCACCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCg                                                >  2:713832/1‑140 (MQ=255)

                                                                                       tGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGt                                              <  2:391233/140‑1 (MQ=255)

                                                                                         gTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTAt                                            <  1:715860/140‑1 (MQ=255)

                                                                                             tAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGt                                        <  2:181261/140‑1 (MQ=255)

                                                                                                  aaCACCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGat                                   <  1:109450/140‑1 (MQ=255)

                                                                                                   acacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGGATCATTCTTTTTGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCATTGGGatc                                  >  2:778758/1‑140 (MQ=255)

                                                                                                    cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTTTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca                                 >  1:477124/1‑140 (MQ=255)

                                                                                                    cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca                                 >  2:492568/1‑140 (MQ=255)

                                                                                                    cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca                                 >  2:213073/1‑140 (MQ=255)

                                                                                                    cacCATCTATCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTGTATAGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGatca                                 >  2:672704/1‑140 (MQ=255)

                                                                                                            aTCCGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGCATCAGTGGGATCATCATTGAc                         >  2:598742/1‑140 (MQ=255)

                                                                                                              ccGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCAtt                            >  2:284671/1‑135 (MQ=255)

                                                                                                              ccGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCAtt                            <  1:284671/135‑1 (MQ=255)

                                                                                                              ccGCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACgt                       >  2:530841/1‑140 (MQ=255)

                                                                                                                gCACATCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACgtgt                     <  1:660298/140‑1 (MQ=255)

                                                                                                                  acaTCTGGAAAAGTTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGTTTCCGTCTGACGTTCTCGCAAATTGCCGATGACGGTATCAGTGGGATCATCATTGACGTGTTg                   >  1:634624/1‑140 (MQ=255)

                                                                                                                   caTCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGa                  <  1:530841/140‑1 (MQ=255)

                                                                                                                     tCTGGAAAAGCTGTGACGGCGGTGTGCTTTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg                <  2:477124/140‑1 (MQ=255)

                                                                                                                     tCTGGAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg                <  2:380623/140‑1 (MQ=255)

                                                                                                                         gAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg                >  2:285322/1‑136 (MQ=255)

                                                                                                                         gAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACg                <  1:285322/136‑1 (MQ=255)

                                                                                                                         gAAAAGCTGTGACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGt            <  1:557018/140‑1 (MQ=255)

                                                                                                                                   gACGGCGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCtt  >  2:676492/1‑140 (MQ=255)

TCACCGGGGTCGCCCTGTGGGGTGGTTCCATTCCCGCGGTTGCGGGTGCCGGGTTTAGTGCCCTCACCCTCGCAATCTTGTTGGGGATGGTTTTAGGCAACACCATCTATCCGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTT  >  NC_000913/2249793‑2250063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A