Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,597,353	A→G	V89A (GTT→GCT)	purC ←	phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,597,353	0	A	G	100.0%	102.6 / NA	30	V89A (GTT→GCT)	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/18); total (12/18)

CGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGA  >  NC_000913/2597219‑2597487

cGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGc                                                                                                                                   <  1:342382/140‑1 (MQ=255)

 gTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGGTCAGCCCAATACCTTCTTTTCTTTCCAAACGTTTTACCCCAGAGCCCGCAGCACGGGTACGCACGAAACACCCACCCCGcc                                                                                                                                  >  2:88810/1‑139 (MQ=255)

                 tGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGGTGGTCTCCATACCCTTTTCGATTTTCCAACGATTTCCCCCAGAGCCACCAGCCCGGGTTGGCGCCACACACTCCGCCCCCACCAAATCCCCCttttt                                                                                                                  >  2:608942/1‑140 (MQ=255)

                    aTGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCAc                                                                                                               <  2:18539/140‑1 (MQ=255)

                            gtTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACAtt                                                                                                       <  1:176336/140‑1 (MQ=255)

                                   aGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATc                                                                                                <  2:352120/140‑1 (MQ=255)

                                           aTCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCa                                                                                        <  1:142441/140‑1 (MQ=255)

                                           aTCGAACAGCGGCGGGGTCAGCTCAAAACCCTCTTTTATTCCAAAACGTTTCACCAGAGAGCCAGCAGCACGGGTACGCACCACACACCCAACCCGCACCCCATCCAGCCTTTTCTCCACACAATCGGGATCGGagagga                                                                                        >  2:109575/1‑138 (MQ=255)

                                               aaCAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACg                                                                                    <  1:109575/140‑1 (MQ=255)

                                                        gggTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTg                                                                           <  2:340486/140‑1 (MQ=255)

                                                              aGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAACAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCgg                                                                     >  1:760077/1‑140 (MQ=255)

                                                                        cttcttCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCt                                                           <  1:437597/140‑1 (MQ=255)

                                                                          tcttcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTc                                                         >  1:270097/1‑140 (MQ=255)

                                                                          tcttcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGAAACCCGCTTc                                                         >  1:23129/1‑140 (MQ=255)

                                                                             tcGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGc                                                      >  2:532545/1‑140 (MQ=255)

                                                                                      aGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCt                                             <  1:100008/140‑1 (MQ=255)

                                                                                      aGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCt                                             <  1:439464/140‑1 (MQ=255)

                                                                                            ttCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAAt                                       >  1:63438/1‑140 (MQ=255)

                                                                                            ttCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAAt                                       >  2:186980/1‑140 (MQ=255)

                                                                                             tCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATg                                      <  2:694820/140‑1 (MQ=255)

                                                                                               aCCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGAAACCCGCTTCAGCCAGTTTGCTCATAATGaa                                    <  2:23129/140‑1 (MQ=255)

                                                                                                      agCCAGCAGCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTg                             >  2:666080/1‑140 (MQ=255)

                                                                                                         cagcagCACGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAAc                          >  2:435747/1‑140 (MQ=255)

                                                                                                            cagcaCGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttg                       <  2:227777/140‑1 (MQ=255)

                                                                                                               caCGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                    <  1:186980/140‑1 (MQ=255)

                                                                                                               caCGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACttgttg                    <  1:158150/140‑1 (MQ=255)

                                                                                                                 cGGTTACGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTgttgtt                  <  2:273909/140‑1 (MQ=255)

                                                                                                                      aCGCACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGATCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCa             >  1:124614/1‑140 (MQ=255)

                                                                                                                         cACGACACACTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATAc          >  2:645817/1‑140 (MQ=255)

                                                                                                                              cacacTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg     >  1:327190/1‑140 (MQ=255)

                                                                                                                              cacacTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTg     >  2:152764/1‑140 (MQ=255)

                                                                                                                                 acTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGa  <  1:435747/140‑1 (MQ=255)

                                                                                                                                 acTCAGCCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGa  <  1:608942/140‑1 (MQ=255)

CGTTGACCATCGGATCGTGCATGGCGTCGTTTTTCAGGAACAGATCGAACAGCGGCGGGTTCAGCTCAATACCTTCTTCGATTCCAAGACGTTTCACCAGAGAGCCAGCAGCACGGTTACGCACGACACACTCAACCGGCACCATATCCAGCTTTTTCACCAGACATTCGGTATCGGAGAGCAGACGCTCCATTTGAGTCGGGATACCCGCTTCAGCCAGTTTGCTCATAATGAAGTAGTTGAACTTGTTGTTCACCATACCTTTGCGA  >  NC_000913/2597219‑2597487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A