Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,649,467	G→A	R941C (CGT→TGT)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,649,467	0	G	A	100.0%	160.9 / NA	49	R941C (CGT→TGT)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/23); total (26/23)

TGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCAACGGCA  >  NC_000913/2649330‑2649597

tgtAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACAcc                                                                                                                                  >  1:673331/1‑140 (MQ=255)

      cGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGAc                                                                                                                            <  1:145432/140‑1 (MQ=255)

          ccTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCCCCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTCACCTTc                                                                                                                        >  2:222006/1‑140 (MQ=255)

          ccTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTc                                                                                                                        >  1:448021/1‑140 (MQ=255)

            ttCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAACTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCaa                                                                                                                      >  2:240803/1‑140 (MQ=255)

             tCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAAt                                                                                                                     >  2:606371/1‑140 (MQ=255)

             tCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAAt                                                                                                                     >  1:220001/1‑140 (MQ=255)

                   tGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTg                                                                                                               <  2:210655/140‑1 (MQ=255)

                     ttCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGAc                                                                                                             >  1:178775/1‑140 (MQ=255)

                      tCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGAcc                                                                                                            >  1:499116/1‑140 (MQ=255)

                       cGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCg                                                                                                           >  2:20878/1‑140 (MQ=255)

                         ttGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:200895/140‑1 (MQ=255)

                         ttGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:318223/140‑1 (MQ=255)

                           gAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAACTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTaaa                                                                                                       <  1:240803/140‑1 (MQ=255)

                               gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         >  2:49592/1‑134 (MQ=255)

                               gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         >  2:719169/1‑134 (MQ=255)

                               gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:719169/134‑1 (MQ=255)

                               gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTa                                                                                                         <  1:49592/134‑1 (MQ=255)

                               gTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATc                                                                                                   >  2:270104/1‑140 (MQ=255)

                                          cgcCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTg                                                                                        >  1:438841/1‑140 (MQ=255)

                                           gcCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTgc                                                                                       <  1:270104/140‑1 (MQ=255)

                                            ccTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGGAAATATCGTAAATGTCTgcg                                                                                      >  2:185450/1‑140 (MQ=255)

                                                     cGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGt                                                                             >  2:35436/1‑140 (MQ=255)

                                                            atTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGAc                                                                      <  2:499116/140‑1 (MQ=255)

                                                                aCGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATa                                                                                 <  1:558053/125‑1 (MQ=255)

                                                                aCGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATa                                                                                 >  2:558053/1‑125 (MQ=255)

                                                                  gTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATGTCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGa                                                                >  2:63610/1‑140 (MQ=255)

                                                                     aTTGACCGGCGGTTTTCCCCCACGGTTTTGCTCATCACCATCGCCACCCGAACCCAGCGCTGCCCGACACCCCTCACCTTTAATAACCTCACCGTAAATATCGTAAATGTCTGCGCCATAACGTTTCTGACCAAAAAAcc                                                             >  2:209938/1‑139 (MQ=255)

                                                                       tGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGcc                                                           <  1:35436/140‑1 (MQ=255)

                                                                            ggcggTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCa                                                      <  2:220001/140‑1 (MQ=255)

                                                                                 tttACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATGTCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGAt                                                 <  1:63610/140‑1 (MQ=255)

                                                                                 tttACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGAt                                                 <  1:209938/140‑1 (MQ=255)

                                                                                           cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGc                                            <  1:386308/135‑1 (MQ=255)

                                                                                           cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGc                                            >  2:386308/1‑135 (MQ=255)

                                                                                           cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:336869/1‑140 (MQ=255)

                                                                                           cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:557928/1‑140 (MQ=255)

                                                                                           cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:542383/1‑140 (MQ=255)

                                                                                           cGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGAc                                       >  1:769585/1‑140 (MQ=255)

                                                                                              ttCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTa                                    <  1:185374/140‑1 (MQ=255)

                                                                                               tCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAg                                   >  1:119969/1‑140 (MQ=255)

                                                                                                cAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGt                                  <  2:557928/140‑1 (MQ=255)

                                                                                                      atcaCCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTaa                            >  2:793530/1‑140 (MQ=255)

                                                                                                        caCCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAata                          <  1:793530/140‑1 (MQ=255)

                                                                                                        caCCATCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAata                          >  1:70305/1‑140 (MQ=255)

                                                                                                             tCGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAg                     >  2:755179/1‑140 (MQ=255)

                                                                                                              cGCCACCGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGc                    <  2:448021/140‑1 (MQ=255)

                                                                                                                   ccGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCAcacc               <  1:334094/140‑1 (MQ=255)

                                                                                                                    cGAAACGCAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCAcacca              >  1:202333/1‑140 (MQ=255)

                                                                                                                           cAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCaa       <  2:336869/140‑1 (MQ=255)

                                                                                                                           cAGAGCTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCaa       <  2:769585/140‑1 (MQ=255)

                                                                                                                                cTGCCAGACACCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCAACGGCa  <  1:755179/140‑1 (MQ=255)

TGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCACGTTTCAGCTCATCACCATCGCCACCGAAACGCAGAGCTGCCAGACGCCCCTGACCTTCAATAACCTGACCGTAAATATCGTAAATGTCTGCGCCATAGCGTTTCTGACCAAAGAACGCCTGCCACGGATCTGGCGTGACGTAGTCAGTAATATTCAGCACACCACTATCAACGGCA  >  NC_000913/2649330‑2649597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A