Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,660,213	T→C	N26S (AAC→AGC)	iscU ←	scaffold protein for iron‑sulfur cluster assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,660,213	0	T	C	100.0%	100.3 / NA	29	N26S (AAC→AGC)	iscU	scaffold protein for iron‑sulfur cluster assembly
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/19); total (10/19)

TGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTC  >  NC_000913/2660082‑2660344

tGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCtcgtcg                                                                                                                             <  1:104469/140‑1 (MQ=255)

tGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCtcgtcg                                                                                                                             <  1:815289/140‑1 (MQ=255)

  gAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCgtt                                                                                                                           >  2:154661/1‑140 (MQ=255)

         tAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCa                                                                                                                    >  2:568373/1‑140 (MQ=255)

           gCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCaaa                                                                                                                  <  1:707761/140‑1 (MQ=255)

                aCCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAAc                                                                                                             <  2:293993/140‑1 (MQ=255)

                aCCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAAc                                                                                                             <  1:72820/140‑1 (MQ=255)

                            aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAgg                                                                                                                <  1:9660/125‑1 (MQ=255)

                            aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAgg                                                                                                                >  2:9660/1‑125 (MQ=255)

                            aGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTAcgcg                                                                                                 <  1:179928/140‑1 (MQ=255)

                                                       aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTGATGGTCGATAACttttt                                                                      <  1:568373/140‑1 (MQ=255)

                                                       aCCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACttttt                                                                      >  2:780727/1‑140 (MQ=255)

                                                                 ttGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGc                                                            >  2:534662/1‑140 (MQ=255)

                                                                 ttGACTTTAATCTGCAACTTCATCACCTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGc                                                            <  1:580373/140‑1 (MQ=255)

                                                                  tGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATTGTCGATAACTTTTTCGCTGTAAGcc                                                           >  2:460159/1‑140 (MQ=255)

                                                                    aCTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCAt                                                         >  2:638727/1‑140 (MQ=255)

                                                                     cTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCAtt                                                        <  1:252629/140‑1 (MQ=255)

                                                                     cTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCAtt                                                        <  1:154661/140‑1 (MQ=255)

                                                                               cAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATtctc                                              >  2:9757/1‑140 (MQ=255)

                                                                                         aCGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATTGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGa                                    <  1:460159/140‑1 (MQ=255)

                                                                                           gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:780727/140‑1 (MQ=255)

                                                                                           gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:714323/140‑1 (MQ=255)

                                                                                           gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:534662/140‑1 (MQ=255)

                                                                                           gTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATa                                  <  1:193001/140‑1 (MQ=255)

                                                                                                                  ccaTGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCg           <  1:638727/140‑1 (MQ=255)

                                                                                                                     tGCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATg        <  1:472127/140‑1 (MQ=255)

                                                                                                                      gCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGa          <  1:742536/137‑1 (MQ=255)

                                                                                                                      gCCGCTGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGa          >  2:742536/1‑137 (MQ=255)

                                                                                                                           tGCCGACGCTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTc  >  2:391156/1‑140 (MQ=255)

TGGAAGCGATAGCGGAACCGCAGCCGTAAGTTTTAAAACGCGCGTCTTCAATGATACCTTCATCGTTGACTTTAATCTGCAACTTCATCACGTCGCCACAGGCCGGTGCCCCCACCATGCCGCTGCCGACGTTCTCGTCGTTGTTGTCAAAGGAACCCACGTTACGCGGATTCTCGTAATGGTCGATAACTTTTTCGCTGTAAGCCATTATAAATTCTCCTGATTCCGATACCGATTAATGATGAGCCCATTCGATGCTGTTC  >  NC_000913/2660082‑2660344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A