Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,057	A→G	V165A (GTC→GCC)	pgpC ←	phosphatidylglycerophosphatase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,057	0	A	G	100.0%	145.2 / NA	42	V165A (GTC→GCC)	pgpC	phosphatidylglycerophosphatase C
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/22); total (20/22)

CAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGACCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCGAAATAAACCGCTTCAACCAG  >  NC_000913/2697921‑2698178

cAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCt                                                                                                                        >  1:405573/1‑140 (MQ=255)

  gTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCttt                                                                                                                      >  2:761556/1‑140 (MQ=255)

  gTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCttt                                                                                                                      >  1:279504/1‑140 (MQ=255)

    tGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTc                                                                                                                    <  1:94425/140‑1 (MQ=255)

    tGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTc                                                                                                                    >  1:516250/1‑140 (MQ=255)

       tGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATg                                                                                                                 <  1:761556/140‑1 (MQ=255)

              cACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGa                                                                                                          >  2:484892/1‑140 (MQ=255)

               aCCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGAc                                                                                                         <  2:100951/140‑1 (MQ=255)

                         gTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGt                                                                                               <  2:405573/140‑1 (MQ=255)

                           tACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCa                                                                                             >  2:817328/1‑140 (MQ=255)

                           tACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCa                                                                                             >  2:461817/1‑140 (MQ=255)

                            aCTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCaa                                                                                            <  2:516250/140‑1 (MQ=255)

                             cTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAAt                                                                                           <  1:819366/140‑1 (MQ=255)

                               cGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATAc                                                                                         <  1:620934/140‑1 (MQ=255)

                                  cAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCa                                                                                      <  1:335623/140‑1 (MQ=255)

                                   aaCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCaa                                                                                     <  1:282909/140‑1 (MQ=255)

                                   aaCGATGCTGACAGAAATAAAGCAGCGGATTGTACTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCaa                                                                                     <  2:128403/140‑1 (MQ=255)

                                    aCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCaa                                                                                            <  1:184770/132‑1 (MQ=255)

                                    aCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCaa                                                                                            >  2:184770/1‑132 (MQ=255)

                                    aCGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAac                                                                                    >  1:529131/1‑140 (MQ=255)

                                            gACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAAt                                                                                           <  1:312818/125‑1 (MQ=255)

                                            gACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAAt                                                                                           >  2:312818/1‑125 (MQ=255)

                                                 aaaTAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACg                                                                       <  2:272456/140‑1 (MQ=255)

                                                            cGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            >  1:685112/1‑140 (MQ=255)

                                                            cGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            >  2:461156/1‑140 (MQ=255)

                                                             ggATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggct                                                           <  1:140572/140‑1 (MQ=255)

                                                                  gTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGa                                                      <  1:817328/140‑1 (MQ=255)

                                                                  gTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGa                                                      <  1:461817/140‑1 (MQ=255)

                                                                             tGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            <  2:401234/123‑1 (MQ=255)

                                                                             tGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTtggc                                                            >  1:401234/1‑123 (MQ=255)

                                                                             tGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAAcc                                           >  2:410554/1‑140 (MQ=255)

                                                                                 gCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCcgcg                                       <  2:685112/140‑1 (MQ=255)

                                                                                  cTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCgg                                      >  1:784737/1‑140 (MQ=255)

                                                                                            tGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCgg                                      <  1:365458/130‑1 (MQ=255)

                                                                                            tGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCgg                                      >  2:365458/1‑130 (MQ=255)

                                                                                                cAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             >  2:525672/1‑135 (MQ=255)

                                                                                                cAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             <  1:525672/135‑1 (MQ=255)

                                                                                                 aGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGTAGCCAGGGCGTATc                       >  2:167244/1‑140 (MQ=255)

                                                                                                  gCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCg                      >  2:57225/1‑140 (MQ=255)

                                                                                                        gCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             >  1:793387/1‑127 (MQ=255)

                                                                                                        gCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAggg                             <  2:793387/127‑1 (MQ=255)

                                                                                                                      ttGCGCTCCAGTTGTGCGGCCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCGAAATAAACCGCTTCAACCAg  <  1:376531/140‑1 (MQ=255)

CAGTTGCTGGAGTTCACCGCGCGGGGTTACTCGCCAACGATGCTGACAGAAATAAAGCAGCGGATTGTCCTGATTACTGTCGCTATAGCCACTGTACAGCCGCAGCGGAGTGCCGATTTTGCGCTCCAGTTGTGCGACCTTTTCATGTCCCAGACAACGCATCGTCAATACCCAACCACCATAGCCACGCTGAATTTGGCTGGCGATAAGATTAACCCGCGGCAGCCAGGGCGTATCGAAATAAACCGCTTCAACCAG  >  NC_000913/2697921‑2698178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A