Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,707,964	T→C	N264S (AAC→AGC)	rseB ←	anti‑sigma factor stabilizing protein RseB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,707,964	0	T	C	100.0%	102.0 / NA	30	N264S (AAC→AGC)	rseB	anti‑sigma factor stabilizing protein RseB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/17); total (13/17)

AATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAAT  >  NC_000913/2707831‑2708098

aaTGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCg                                                                                                                                  <  2:573469/140‑1 (MQ=255)

  tGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGTCGTAGCGCGGTTAACGCTTACCgag                                                                                                                                <  1:514452/140‑1 (MQ=255)

         cGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCgaga                                                                                                                               >  2:654615/1‑134 (MQ=255)

         cGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCgaga                                                                                                                               <  1:654615/134‑1 (MQ=255)

             gTTTGCGGCGGCTGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCCATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGaataa                                                                                                                     <  1:223274/140‑1 (MQ=255)

                  cggcggCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGt                                                                                                                <  1:418623/140‑1 (MQ=255)

                    gcggcAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCg                                                                                                              <  2:602677/140‑1 (MQ=255)

                                  aCAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGa                                                                                                <  2:369478/140‑1 (MQ=255)

                                   cAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAt                                                                                               <  2:773055/140‑1 (MQ=255)

                                    aaTGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAtt                                                                                              <  2:442840/140‑1 (MQ=255)

                                     aTGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAtt                                                                                              <  2:601234/139‑1 (MQ=255)

                                     aTGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAtt                                                                                              >  1:601234/1‑139 (MQ=255)

                                             ttCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCa                                                                                     >  1:828824/1‑140 (MQ=255)

                                                ggCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgt                                                                                  >  1:15416/1‑140 (MQ=255)

                                                   gTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt                                                                               <  2:397760/140‑1 (MQ=255)

                                                                 ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt                                                                               >  1:773326/1‑126 (MQ=255)

                                                                 ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCAtgttgt                                                                               <  2:773326/126‑1 (MQ=255)

                                                                 ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGc                                                                 >  1:522237/1‑140 (MQ=255)

                                                                 ttGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGc                                                                 >  1:332182/1‑140 (MQ=255)

                                                                   gTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCgg                                                               >  1:771169/1‑140 (MQ=255)

                                                                                     ccGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAAc                                             <  2:332182/140‑1 (MQ=255)

                                                                                       ggTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACtt                                           <  2:522237/140‑1 (MQ=255)

                                                                                       ggTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACtt                                           <  2:771169/140‑1 (MQ=255)

                                                                                         tGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCg                                         >  1:591509/1‑140 (MQ=255)

                                                                                                                     cGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTg                >  2:562386/1‑137 (MQ=255)

                                                                                                                     cGTAGCGCGGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTg                <  1:562386/137‑1 (MQ=255)

                                                                                                                          cgcgGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAg        >  1:617687/1‑140 (MQ=255)

                                                                                                                            cgGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCt      >  1:811/1‑140 (MQ=255)

                                                                                                                            cgGTTAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCt      >  1:501728/1‑140 (MQ=255)

                                                                                                                                tAACGCTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAAt  <  2:811/140‑1 (MQ=255)

AATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTTCGCTAAAACCCTGTGGCAACCAGGTTGGCGTCCAGCTGAAT  >  NC_000913/2707831‑2708098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A